Primeiro Registro de Zoophthora radicans (Entomophthorales: Entomophthoraceae) em Adultos da Ampola-da-Erva-Mate, Gyropsylla spegazziniana Lizer &amp; Trelles (Hemiptera: Psyllidae), no Brasil

Alves, L. F. A.; Leite, L. G.; Oliveira, Daian Guilherme Pinto de

doi:10.1590/s1519-566x2009000500025

Cited by 10 publications

(9 citation statements)

References 5 publications

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“…As for the entomopathogens, Sosa-Gómez et al (1994) reported, in yerba mate cultures in Corrientes Province, Argentina, infection of the Zoophthora radicans (Brefeld) fungus in up to 90% of the ampoule-of-yerba-mate adults. In Brazil, Alves et al (2009) also reported this association in a yerba mate culture in the municipality of Cascavel, State of Paraná, with similar levels of occurrence in this population. These are the only records of entomopathogens on G. spegazziniana.…”

Section: Abstract: Ilex Paraguariensis; Entomopathogenic Fungi; Biolosupporting

confidence: 55%

Susceptibility of Gyropsylla spegazziniana (Lizer & Trelles) (Hemiptera: Psyllidae) to Beauveria bassiana (Bals.) Vuill.

et al. 2013

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Gyropsylla spegazziniana is one of the most prominent pest insects of yerba mate culture in all production regions in Brazil, Argentina and Paraguay. Natural enemies have been recorded on G. spegazziniana nymphs and adults, including the Zoophthora radicans entomopathogenic fungus. Since there are no reports of Beauveria bassiana, the aim of this study was to register its pathogenicity with respect to this insect in a laboratory setting. Yerba mate branches were kept in glass flasks with water and we infested each leaf with 20 newly-emerged nymphs. We prepared three replicates per treatment. We sprayed conidia suspensions (1 × 109 conidia/mL) onto the branches, which we transferred to cages and kept in an acclimatized room (26 ± 1°C, 70 ± 10% of relative humidity (R.H.) with a photophase of 14 hours). We evaluated insect mortality daily, and after five days we observed a high level of pathogenicity due to the presence of the fungus, that could be visually observed on the cadaver. The total mortality varied from 25 to 70% (respectively for Unioeste 4 and CG 716) and the confirmed mortality was 30% for Unioeste 52, revealing the fungus' potential. However, more studies are necessary in order to evaluate the strains of this fungus, as well as other species.

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Section: Abstract: Ilex Paraguariensis; Entomopathogenic Fungi; Biolosupporting

confidence: 55%

Susceptibility of Gyropsylla spegazziniana (Lizer & Trelles) (Hemiptera: Psyllidae) to Beauveria bassiana (Bals.) Vuill.

et al. 2013

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“…In this context, studies conducted in Brazil have shown the potential of vegetal extracts (BARZOTTO, 2010;HAAS et al, 2010), and the entomopathogenic fungus Zoophthora radicans (Brefeld) has been observed on 90% of G. spegazziniana cadavers in Paraguay tea plantations in Brazil and Argentina (ALVES et al, 2009;SOSA-GÓMEZ et al, 1994). Additionally, the susceptibility of G. spegazziniana to Beauveria bassiana isolates was recently demonstrated for the first time, revealing encouraging results (ALVES et al, 2013).…”

Section: Introductionmentioning

confidence: 95%

Characterization and activity of entomopathogenic fungi isolates against “paraguay tea ampul” (Gyropsylla spegazziniana) (Lizer & Trelles) (Hemiptera: Psyllidae)

et al. 2015

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Paraguay tea is a native crop of southern Brazil and is also socioeconomically important in Paraguay and Argentina. One of the main pests of this crop is Gyropsylla spegazziniana (Hemiptera: Psyllidae). As there are no registered insecticides for this pest in Brazil, the use of entomopathogenic fungi is an alternative method for its control. This study aimed to evaluate and characterize isolates of the entomopathogenic fungi Beauveria spp., Metarhizium anisopliae, Isaria spp. and Lecanicillium spp. on G. spegazziniana. For this purpose, 5 th instar nymphs were transferred to Paraguay tea seedlings, followed by spraying of conidial suspensions (1 × 10 9 conidia mL -1 ) and then placing the seedlings in polyvinyl chloride (PVC) cages and maintaining them in a temperature-controlled room (26 ± 1°C, 12h00 photophase, 60 ± 10% R.H.). Insect mortality was evaluated daily for 10 days, and the selected isolates were characterized according to their vegetative growth, conidial production in synthetic culture medium and rice and insecticidal activity as well as through molecular analyses, including sequencing the rDNA-ITS region and RAPD analysis. The genus Beauveria spp. was the most efficient, particularly the Unioeste 44 isolate, which caused the greatest total mortality of G. spegazziniana (81.7%) and showed among the highest conidial production levels on rice, indicating a significant potential to be used in an integrated management program for this pest. Molecular analysis of the rDNA-ITS region allowed the isolates to be identified as B. bassiana and B. brongniartii, and RAPD markers were found to be associated with virulence. Key words: Biological control, Ilex paraguariensis, rDNA-ITS ResumoA erva-mate (Ilex paraguariensis St. Hil.) é uma cultura nativa do Sul do Brasil, tendo grande importância socioeconômica também para o Paraguai e Argentina. Uma das principais pragas da cultura é a Gyropsylla spegazziniana (Hemiptera: Psyllidae), e pelo fato de não existirem inseticidas registrados para essa praga no Brasil, o uso de fungos entomopatogênicos pode ser uma alternativa para o seu controle. O objetivo desse trabalho foi caracterizar isolados dos fungos entomopatogênicos Beauveria spp., Metarhizium anisopliae, Isaria spp. e Lecanicillium spp. sobre G. spegazziniana. Para tal, ninfas de 5 o ínstar foram transferidas para mudas de erva-mate, seguido da pulverização das suspensões de conídios (1 × 10 9 conídios mL -1 ), e então as mudas foram acondicionadas em gaiolas de PVC (cloreto de polivinila), e mantidas em sala climatizada (26 ± 1°C; 12h00 de fotofase e U.R. 60 ± 10%). A mortalidade dos insetos foi avaliada diariamente, por 10 dias, e os isolados selecionados foram comparados entre si por meio do crescimento vegetativo, produção de conídios em meio sintético e em arroz, atividade inseticida e análises moleculares, utilizando-se o sequenciamento da região rDNA-ITS e marcadores RAPD. O gênero Beauveria spp. foi o mais eficiente, em especial o isolado Unioeste 44 que apresentou a maior mortalidade total s...

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“…radicans is one of the most common and globally distributed entomophthoralean entomopathogens causing epizootics under natural conditions (Papierok and Hajek, 1997), and infects a wide range of hosts (Humber, 1989). The first report of Z. radicans in Brazil was made by Hoffmann et al (1979) on the soybean caterpillar Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae), and recently Alves et al (2009) observed a natural epizootic of this fungus causing 90% mortality on the psyllid Gyropsylla spegazziniana Lizer & Trelles (Hemiptera: Psyllidae) in a commercial Paraguay tea plantation. Besides these reports, the ARS Collection of Entomopathogenic Fungal Cultures holds some strains of Z. radicans collected in Brazil mostly on insect species belonging to Cicadellidae.…”

Section: Introductionmentioning

confidence: 99%

Natural occurrence of Zoophthora radicans (Entomophthorales: Entomophthoraceae) on Thaumastocoris peregrinus (Heteroptera: Thaumastocoridae), an invasive pest recently found in Brazil

Mascarin

Duarte

Brandão

et al. 2012

Journal of Invertebrate Pathology

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Occurrence of Zoophthora radicans infecting nymphs and adults of Thaumastocoris peregrinus Carpintero and Dellape, 2006 is reported in Brazil. This is a new record of host for this fungal species and the first fungal pathogen associated with this pest worldwide. Infection of Z. radicans on T. peregrinus populations on commercial Eucalyptus plantation (Eucalyptus spp.) reached up to 100%, and low insect densities were associated with high levels of fungal infection in three out of seven plots. This pathogen seems to be virulent against T. peregrinus and may play an important role in population regulations of this invasive pest through naturally induced epizootics.

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Primeiro Registro de Zoophthora radicans (Entomophthorales: Entomophthoraceae) em Adultos da Ampola-da-Erva-Mate, Gyropsylla spegazziniana Lizer & Trelles (Hemiptera: Psyllidae), no Brasil

Cited by 10 publications

References 5 publications

Susceptibility of Gyropsylla spegazziniana (Lizer & Trelles) (Hemiptera: Psyllidae) to Beauveria bassiana (Bals.) Vuill.

Susceptibility of Gyropsylla spegazziniana (Lizer & Trelles) (Hemiptera: Psyllidae) to Beauveria bassiana (Bals.) Vuill.

Characterization and activity of entomopathogenic fungi isolates against “paraguay tea ampul” (Gyropsylla spegazziniana) (Lizer & Trelles) (Hemiptera: Psyllidae)

Natural occurrence of Zoophthora radicans (Entomophthorales: Entomophthoraceae) on Thaumastocoris peregrinus (Heteroptera: Thaumastocoridae), an invasive pest recently found in Brazil

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