Intraespecific variation on the aedeagus of Anopheles oswaldoi (Peryassú) (Diptera: Culicidae)

Motoki, Maysa Tiemi; Santos, Cecília Luiza Simões; Sallum, Maria Anice Mureb

doi:10.1590/s1519-566x2009000100017

Cited by 6 publications

(5 citation statements)

References 14 publications

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“…konderi in Colombia, Ecuador and Peru. These data, combined with previous studies [ 7 , 13 , 14 , 25 , 63 ], indicated that there were three species in the An . oswaldoi complex ( An .…”

Section: Introductionsupporting

confidence: 79%

Molecular taxonomy and evolutionary relationships in the Oswaldoi-Konderi complex (Anophelinae: Anopheles: Nyssorhynchus) from the Brazilian Amazon region

2018

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Recent studies have shown that Anopheles oswaldoi sensu lato comprises a cryptic species complex in South America. Anopheles konderi, which was previously raised to synonymy with An. oswaldoi, has also been suggested to form a species complex. An. oswaldoi has been incriminated as a malaria vector in some areas of the Brazilian Amazon, Colombia, Peru and Venezuela, but was not recognized as a vector in the remaining regions in its geographic distribution. The role of An. konderi as a malaria vector is unknown or has been misattributed to An. oswaldoi. The focus of this study was molecular identification to infer the evolutionary relationships and preliminarily delimit the geographic distribution of the members of these complexes in the Brazilian Amazon region. The specimens were sampled from 18 localities belonging to five states in the Brazilian Amazon and sequenced for two molecular markers: the DNA barcode region (COI gene of mitochondrial DNA) and Internal Transcribed Spacer 2 (ITS2 ribosomal DNA). COI (83 sequences) and ITS2 (27 sequences) datasets generated 43 and 10 haplotypes, respectively. Haplotype networks and phylogenetic analyses generated with the barcode region (COI gene) recovered five groups corresponding to An. oswaldoi s.s., An. oswaldoi B, An. oswaldoi A, An. konderi and An. sp. nr. konderi; all pairwise genetic distances were greater than 3%. The group represented by An. oswaldoi A exhibited three strongly supported lineages. The molecular dating indicated that the diversification process in these complexes started approximately 2.8 Mya, in the Pliocene. These findings confirm five very closely related species and present new records for these species in the Brazilian Amazon region. The paraphyly observed for the An. oswaldoi complex suggests that An. oswaldoi and An. konderi complexes may comprise a unique species complex named Oswaldoi-Konderi. Anopheles oswaldoi B may be a potential malaria vector in the extreme north of the Brazilian Amazon, whereas evidence of sympatry for the remaining species in other parts of the Brazilian Amazon (Acre, Amazonas, Pará and Rondônia) precluded identification of probable vectors in those areas.

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“…konderi in Colombia, Ecuador and Peru. These data, combined with previous studies [ 7 , 13 , 14 , 25 , 63 ], indicated that there were three species in the An . oswaldoi complex ( An .…”

Section: Introductionsupporting

confidence: 79%

Molecular taxonomy and evolutionary relationships in the Oswaldoi-Konderi complex (Anophelinae: Anopheles: Nyssorhynchus) from the Brazilian Amazon region

2018

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“…oswaldoi s.s . has only been confirmed in the Brazilian States of Espírito Santo, Rio de Janeiro and São Paulo in Brazil [12,24]. Pinault and Hunter [25] recently reported three COI sequences of An.…”

Section: Introductionmentioning

confidence: 99%

Systematics of the Oswaldoi Complex (Anopheles, Nyssorhynchus) in South America

et al. 2013

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BackgroundEffective malaria control relies on accurate identification of those Anopheles mosquitoes responsible for the transmission of Plasmodium parasites. Anopheles oswaldoi s.l. has been incriminated as a malaria vector in Colombia and some localities in Brazil, but not ubiquitously throughout its Neotropical range. This evidence together with variable morphological characters and genetic differences supports that An. oswaldoi s.l. compromises a species complex. The recent fully integrated redescription of An. oswaldoi s.s. provides a solid taxonomic foundation from which to molecularly determine other members of the complex.MethodsDNA sequences of the Second Internal Transcribed Spacer (ITS2 - rDNA) (n = 192) and the barcoding region of the Cytochrome Oxidase I gene (COI - mtDNA) (n = 110) were generated from 255 specimens of An. oswaldoi s.l. from 33 localities: Brazil (8 localities, including the lectotype series of An. oswaldoi), Ecuador (4), Colombia (17), Trinidad and Tobago (1), and Peru (3). COI sequences were analyzed employing the Kimura-two-parameter model (K2P), Bayesian analysis (MrBayes), Mixed Yule-Coalescent model (MYC, for delimitation of clusters) and TCS genealogies.ResultsSeparate and combined analysis of the COI and ITS2 data sets unequivocally supported four separate species: two previously determined (An. oswaldoi s.s. and An. oswaldoi B) and two newly designated species in the Oswaldoi Complex (An. oswaldoi A and An. sp. nr. konderi). The COI intra- and inter-specific genetic distances for the four taxa were non-overlapping, averaging 0.012 (0.007 to 0.020) and 0.052 (0.038 to 0.064), respectively. The concurring four clusters delineated by MrBayes and MYC, and four independent TCS networks, strongly confirmed their separate species status. In addition, An. konderi of Sallum should be regarded as unique with respect to the above. Despite initially being included as an outgroup taxon, this species falls well within the examined taxa, suggesting a combined analysis of these taxa would be most appropriate.ConclusionsThrough novel data and retrospective comparison of available COI and ITS2 DNA sequences, evidence is shown to support the separate species status of An. oswaldoi s.s., An. oswaldoi A and An. oswaldoi B, and at least two species in the closely related An. konderi complex (An. sp. nr. konderi, An. konderi of Sallum). Although An. oswaldoi s.s. has never been implicated in malaria transmission, An. oswaldoi B is a confirmed vector and the new species An. oswaldoi A and An. sp. nr. konderi are circumstantially implicated, most likely acting as secondary vectors.

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“…Para el soporte entomológico, se realizó montaje en lámina-laminilla de las alas y pata posterior de un 5% de los especímenes reportados como A. nuneztovari s.l. (2.017), de todos los identificados como A. darlingi (16), los especímenes cuya identificación por morfología no pudo ser definida con certeza (35) y a los identificados como A. oswaldoi (2).…”

Section: Materiales Y Métodos Recolección E Identificación De Mosquitosunclassified

“…13,14 Por lo anterior, y para lograr una correcta identificación de las especies problemáticas de Anopheles, se han empleado varias estrategias, como el análisis morfológico de estados inmaduros del mosquito, 13 los huevos, la quetotaxia de las larvas de cuarto estado, 15 y la observación de la genitalia de machos. 16 Para la identificación de especímenes del grupo Oswaldoi, se ha utilizado la morfología geométrica, la cual permite asignar un individuo a un grupo determinado o especie más probable, mediante análisis estadísticos de puntos anatómicos. 17 En los últimos años, se han implementado pruebas moleculares basadas en la Reacción en Cadena de la Polimerasa-PCR; 18-20 entre estas, una metodología que ha demostrado ser efectiva y sencilla, es una PCR-RFLP (Longitud de los Fragmentos de Restricción-RFLP), que posibilita la confirmación de la identificación de varias especies de mosquitos del género Anopheles.…”

Section: Introductionunclassified

confirmación molecular de especie en especímenes Anopheles recolectados en el corregimiento de Juan José,Puerto Libertador, Córdoba, colombia

et al. 2012

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Introducción La dificultad en la identificación morfológica de las hembras de varias especies del subgénero Nyssorhynchus ha sido documentada y se debe a la similitud morfológica interespecie, variación intraespecífica y la existencia de especies crípticas. Por ello, se han utilizado pruebas moleculares como la PCR-RFLP-ITS2, que permiten confirmar la identidad de las especies presentes en regiones endémicas. obJetivo Confirmar la identificación de mosquitos del género Anopheles recolectados en Juan José, Puerto Libertador, Córdoba, como aporte a un inventario de especies en la localidad. Materiales y métodos De 2.070 hembras de Anopheles identificados por caracteres morfológicos, 513 (24,8%) se confirmaron molecularmente mediante una PCR-RFLP-ITS2. Resultados Las especies identificadas por morfología presentes en Juan José fueron: Anopheles (Nyssorhynchus) nuneztovari s.l. Gabaldon (97,44%), Anopheles (Nys.) darlingi Root (0,77%) y Anopheles (Nys.) oswaldoi s.l. Peryassu (0,10%); el resto de especímenes (1,69%) se definieron como pertenecientes a una de las especies del grupo Oswaldoi. La confirmación molecular permitió determinar la presencia de sólo dos especies entre los especímenes analizados, 99,23% A. (Nys.) nuneztovari s.l. y 0,77% A. (Nys.) darlingi. Conclusión Las dos especies de Anopheles encontradas en Juan José, durante el muestreo, son consideradas vectores primarios en Colombia; esta información es importante para dirigir los esfuerzos de control vectorial. Adicionalmente, los resultados reiteran previos reportes sobre la dificultad en la identificación morfológica de A. nuneztovari s.l. y la importancia de utilizar pruebas moleculares en su confirmación de especie.

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Intraespecific variation on the aedeagus of Anopheles oswaldoi (Peryassú) (Diptera: Culicidae)

Cited by 6 publications

References 14 publications

Molecular taxonomy and evolutionary relationships in the Oswaldoi-Konderi complex (Anophelinae: Anopheles: Nyssorhynchus) from the Brazilian Amazon region

Molecular taxonomy and evolutionary relationships in the Oswaldoi-Konderi complex (Anophelinae: Anopheles: Nyssorhynchus) from the Brazilian Amazon region

Systematics of the Oswaldoi Complex (Anopheles, Nyssorhynchus) in South America

confirmación molecular de especie en especímenes Anopheles recolectados en el corregimiento de Juan José,Puerto Libertador, Córdoba, colombia

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