Molecular taxonomy and evolutionary relationships in the Oswaldoi-Konderi complex (Anophelinae: Anopheles: Nyssorhynchus) from the Brazilian Amazon region

Saraiva, José Ferreira; Souto, Raimundo Nonato Picanço; Scarpassa, Vera Margarete

doi:10.1371/journal.pone.0193591

Cited by 16 publications

(21 citation statements)

References 96 publications

(160 reference statements)

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“…konderi of Sallum” (GenBank accession: KF809030–033) in Ruiz-Lopez et al [ 61 ] and “ An. konderi ” (GenBank accession: JF923716) in Saraiva et al [ 34 ] all belong to this lineage. This lineage was collected in rural settlements in Acre as well as from sites in Amapá.…”

Section: Resultsmentioning

confidence: 99%

“…konderi and Ny. oswaldoi complexes indicate that these should be considered a unique species complex named Oswaldoi-Konderi [ 34 ]. There are now considered to be five species in this Oswaldoi–Konderi Complex, which include Ny.…”

Section: Discussionmentioning

confidence: 99%

“…oswaldoi A appears to be found through much of the Amazon River basin (Amazonas, Acre, Pará, Rondônia), Ny. oswaldoi B was not identified at the collection sites, although it has previously been identified from a site in the northern reaches of the Brazilian Amazon (Santana, Amapá; [ 34 ]). A Ny.…”

Section: Discussionmentioning

confidence: 99%

“…A great variety of potential vectors have been recorded in the deforested areas of the Amazon [ 30 – 32 ], but the frequent occurrence of morphologically indistinguishable sibling species, which form species complexes [ 33 ], means that vectors are often misidentified or cryptic species boundaries go undetected [ 34 – 36 ]. Some vectors, such as Nyssorhynchus triannulatus and Anopheles peryassui , inhabit the forest edge, away from domestic environments and conventional vector control activities, but may be associated with human activity such as deforestation [ 37 ].…”

Section: Introductionmentioning

confidence: 99%

“…DNA barcoding is now seen as an important first step in biodiversity assessment and sorting specimens into tentative species, and COI the marker of choice because of the availability of universal primers that amplify across a diverse range of species, and the relatively low intraspecific and high interspecific divergence that occurs at this locus. It is clear that barcoding approaches are helping to resolve morphologically indistinguishable species within Anophelinae [ 31 , 34 , 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

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Exploring malaria vector diversity on the Amazon Frontier

Bourke

Conn

Oliveira

et al. 2018

Malar J

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BackgroundDeforestation in the Amazon and the social vulnerability of its settler communities has been associated with increased malaria incidence. The feeding biology of the most important malaria vectors in the region, notably Nyssorhynchus darlingi, compounds efforts to control vectors and reduce transmission of what has become known as “Frontier Malaria”. Exploring Anophelinae mosquito diversity is fundamental to understanding the species responsible for transmission and developing appropriate management and intervention strategies for malaria control in the Amazon River basin.MethodsThis study describes Anophelinae mosquito diversity from settler communities affected by Frontier Malaria in the states of Acre, Amazonas and Rondônia by analysing COI gene data using cluster and tree-based species delimitation approaches.ResultsIn total, 270 specimens from collection sites were sequenced and these were combined with 151 reference (GenBank) sequences in the analysis to assist in species identification. Conservative estimates found that the number of species collected at these sites was between 23 (mPTP partition) and 27 (strict ABGD partition) species, up to 13 of which appeared to be new. Nyssorhynchus triannulatus and Nyssorhynchus braziliensis displayed exceptional levels of intraspecific genetic diversity but there was little to no support for putative species complex status.ConclusionsThis study demonstrates that Anophelinae mosquito diversity continues to be underestimated in poorly sampled areas where frontier malaria is a major public health concern. The findings will help shape future studies of vector incrimination and transmission dynamics in these areas and support efforts to develop more effective vector control and transmission reduction strategies in settler communities in the Amazon River basin.Electronic supplementary materialThe online version of this article (10.1186/s12936-018-2483-2) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Exploring malaria vector diversity on the Amazon Frontier

Bourke

Conn

Oliveira

et al. 2018

Malar J

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Using capillary electrophoresis to identify Anopheline species in routine sampling sites

Chabanol,

Romoli,

Talaga

et al. 2024

Ecology and Evolution

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In the Anopheles genus, various mosquito species are able to transmit the Plasmodium parasites responsible for malaria, while others are non‐vectors. In an effort to better understand the biology of Anopheles species and to quantify transmission risk in an area, the identification of mosquito species collected in the field is an essential but problematic task. Morphological identification requires expertise and cannot be checked after processing samples in a destructive treatment, while sequencing of numerous samples is costly. Here, we introduce a method of Species identification via Simple Observation Coupled with Capillary Electrophoresis Technology (SOCCET). This molecular technique of species identification is based on precise determination of ITS2 length combined with a simple visual observation, the colour of mosquito hindleg tip. DNA extracted from field‐collected Anopheles mosquitoes was amplified with universal Anopheles ITS2 primers and analysed with a capillary electrophoresis device, which precisely determines the size of the fragments. We defined windows of amplicon sizes combined with fifth hind tarsus colour, which allows discrimination of the major Anopheles species found in our collections. We validated our parameters via Sanger sequencing of ITS2 amplicons. Using the SOCCET method, we characterised the composition of Anopheles populations in five locations of French Guiana, where we detected a total of nine species. Anopheles braziliensis and Anopheles darlingi were detected in four locations each and represented 13 and 67% of our samples, respectively. The SOCCET method can be particularly useful when working with routine sampling sites with a moderate species diversity, that is, when the number of local species is too high to define species‐specific primers but low enough to avoid individual ITS2 sequencing. This tool will be of interest to evaluate local malaria transmission risk and this approach may be further implemented for other mosquito genera.

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Anopheles (Nyssorhynchus) tadei: A new species of the Oswaldoi-konderi complex (Diptera, Anophelinae) and its morphological and molecular distinctions from An. konderi sensu stricto

Saraiva

Scarpassa

2021

Acta Tropica

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Molecular taxonomy and evolutionary relationships in the Oswaldoi-Konderi complex (Anophelinae: Anopheles: Nyssorhynchus) from the Brazilian Amazon region

Cited by 16 publications

References 96 publications

Exploring malaria vector diversity on the Amazon Frontier

Exploring malaria vector diversity on the Amazon Frontier

Using capillary electrophoresis to identify Anopheline species in routine sampling sites

Anopheles (Nyssorhynchus) tadei: A new species of the Oswaldoi-konderi complex (Diptera, Anophelinae) and its morphological and molecular distinctions from An. konderi sensu stricto

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