Occurrence of Wolbachia in Brazilian samples of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)

Rocha, Lincoln S.; Mascarenhas, Rodrigo de Oliveira; Perondini, André Luiz Paranhos; Selivon, Denise

doi:10.1590/s1519-566x2005000600020

Cited by 26 publications

(21 citation statements)

References 14 publications

(9 reference statements)

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“…All other tephritid species are so far considered as Wolbachia -free or only exhibiting low prevalence infections. Among them, Ceratitis capitata is also considered as Wolbachia -free (Bourtzis et al 1994, Zabalou et al 2004); however, there are two reports from a research group in Latin America discussing the presence of Wolbachia in local populations of the species (Rocha et al 2005, Coscrato et al 2009). The recent study on the Wolbachia presence in Australian fruit flies (Morrow et al 2015) has extended our knowledge on the Wolbachia status of Tephritidae in a relatively unexplored area.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic and symbiont analysis of five members of the B. dorsalis complex (Diptera, Tephritidae): no evidence of chromosomal or symbiont-based speciation events

Augustinos¹,

Drosopoulou²,

Gariou-Papalexiou³

et al. 2015

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The Bactrocera dorsalis species complex, currently comprising about 90 entities has received much attention. During the last decades, considerable effort has been devoted to delimiting the species of the complex. This information is of great importance for agriculture and world trade, since the complex harbours several pest species of major economic importance and other species that could evolve into global threats. Speciation in Diptera is usually accompanied by chromosomal rearrangements, particularly inversions that are assumed to reduce/eliminate gene flow. Other candidates currently receiving much attention regarding their possible involvement in speciation are reproductive symbionts, such as Wolbachia, Spiroplasma, Arsenophonus, Rickettsia and Cardinium. Such symbionts tend to spread quickly through natural populations and can cause a variety of phenotypes that promote pre-mating and/or post-mating isolation and, in addition, can affect the biology, physiology, ecology and evolution of their insect hosts in various ways. Considering all these aspects, we present: (a) a summary of the recently gained knowledge on the cytogenetics of five members of the Bactrocera dorsalis complex, namely Bactrocera dorsalis s.s., Bactrocera invadens, Bactrocera philippinensis, Bactrocera papayae and Bactrocera carambolae, supplemented by additional data from a Bactrocera dorsalis s.s. colony from China, as well as by a cytogenetic comparison between the dorsalis complex and the genetically close species, Bactrocera tryoni, and, (b) a reproductive symbiont screening of 18 different colonized populations of these five taxa. Our analysis did not reveal any chromosomal rearrangements that could differentiate among them. Moreover, screening for reproductive symbionts was negative for all colonies derived from different geographic origins and/or hosts. There are many different factors that can lead to speciation, and our data do not support chromosomal and/or symbiotic-based speciation phenomena in the taxa under study.

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Section: Introductionmentioning

confidence: 99%

Cytogenetic and symbiont analysis of five members of the B. dorsalis complex (Diptera, Tephritidae): no evidence of chromosomal or symbiont-based speciation events

Augustinos¹,

Drosopoulou²,

Gariou-Papalexiou³

et al. 2015

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“…In conclusion, like other South American arthropods (Shoemaker et al ., 2000; Ciociola Jr et al ., 2001; Ono et al ., 2001; Selivon et al ., 2002; Vega et al ., 2002; Dittmar and Whiting, 2004; Heukelbach et al ., 2004; Rocha et al ., 2005; Cônsoli and Katajima, 2006; Souza et al ., 2009), Wolbachia also infects South American terrestrial isopods. The population-based approach we took allowed a more accurate estimation of prevalence rates in these species, and permitted the discovery of a high genetic diversity of Wolbachia isolates.…”

Section: Discussionmentioning

confidence: 99%

First record of Wolbachia in South American terrestrial isopods: prevalence and diversity in two species of Balloniscus (Crustacea, Oniscidea)

et al. 2012

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Wolbachia are endosymbiotic bacteria that commonly infect arthropods, inducing certain phenotypes in their hosts. So far, no endemic South American species of terrestrial isopods have been investigated for Wolbachia infection. In this work, populations from two species of Balloniscus (B. sellowii and B. glaber) were studied through a diagnostic PCR assay. Fifteen new Wolbachia 16S rDNA sequences were detected. Wolbachia found in both species were generally specific to one population, and five populations hosted two different Wolbachia 16S rDNA sequences. Prevalence was higher in B. glaber than in B. sellowii, but uninfected populations could be found in both species. Wolbachia strains from B. sellowii had a higher genetic variation than those isolated from B. glaber. AMOVA analyses showed that most of the genetic variance was distributed among populations of each species rather than between species, and the phylogenetic analysis suggested that Wolbachia strains from Balloniscus cluster within Supergroup B, but do not form a single monophyletic clade, suggesting multiple infections for this group. Our results highlight the importance of studying Wolbachia prevalence and genetic diversity in Neotropical species and suggest that South American arthropods may harbor a great number of diverse strains, providing an interesting model to investigate the evolution of Wolbachia and its hosts.

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“…The DNA fragments were visualized after staining with 5 μg/mL ethidium bromide in a UV transilluminator. Samples of Wolbachia -infected Ceratitis capitata (Rocha et al , 2005) were used as a positive control for the PCR assays. In case of a negative amplification, the sample DNA was tested for amplification of the 28S rDNA using the universal arthropod primers, and samples that were negative were discarded only after changing the DNA concentrations and PCR conditions (Werren et al.…”

Section: Methodsmentioning

confidence: 99%

“…Among the frugivorous tephritid flies, Wolbachia was found to infect species of the genera Rhagoletis (Riegler and Stauffer, 2002; Schuler et al , 2009, 2011, 2013; Arthofer et al , 2009; Drosopoulou et al , 2011; Augustinos et al , 2014), Bactrocera (Kittayapong et al , 2000; Jamnongluk et al , 2002; Liu et al , 2006; Sun et al , 2007; Morrow et al , 2014, 2015), Dacus (Kittayapong et al , 2000), Ceratitis (Rocha et al. , 2005), and Anastrepha (Werren et al , 1995; Selivon et al , 2002; Coscrato, et al , 2009; Cáceres et al , 2009; Marcon et al , 2011; Martínez et al , 2012).…”

Section: Introductionmentioning

confidence: 99%

Wolbachia in guilds of Anastrepha fruit flies (Tephritidae) and parasitoid wasps (Braconidae)

et al. 2016

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The endosymbiont Wolbachia is efficiently transmitted from females to their progenies, but horizontal transmission between different taxa is also known to occur. Aiming to determine if horizontal transmission might have occurred between Anastrepha fruit flies and associated braconid wasps, infection by Wolbachia was screened by amplification of a fragment of the wsp gene. Eight species of the genus Anastrepha were analyzed, from which six species of associated parasitoid wasps were recovered. The endosymbiont was found in seven Anastrepha species and in five species of braconids. The WSP Typing methodology detected eight wsp alleles belonging to Wolbachia supergroup A. Three were already known and five were new ones, among which four were found to be putative recombinant haplotypes. Two samples of Anastrepha obliqua and one sample of Doryctobracon brasiliensis showed multiple infection. Single infection by Wolbachia was found in the majority of samples. The distribution of Wolbachia harboring distinct alleles differed significantly between fruit flies and wasps. However, in nine samples of fruit flies and associated wasps, Wolbachia harbored the same wsp allele. These congruences suggest that horizontal transfer of Wolbachia might have occurred in the communities of fruit flies and their braconid parasitoids.

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Occurrence of Wolbachia in Brazilian samples of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)

Cited by 26 publications

References 14 publications

Cytogenetic and symbiont analysis of five members of the B. dorsalis complex (Diptera, Tephritidae): no evidence of chromosomal or symbiont-based speciation events

Cytogenetic and symbiont analysis of five members of the B. dorsalis complex (Diptera, Tephritidae): no evidence of chromosomal or symbiont-based speciation events

First record of Wolbachia in South American terrestrial isopods: prevalence and diversity in two species of Balloniscus (Crustacea, Oniscidea)

Wolbachia in guilds of Anastrepha fruit flies (Tephritidae) and parasitoid wasps (Braconidae)

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