First record of Wolbachia in South American terrestrial isopods: prevalence and diversity in two species of Balloniscus (Crustacea, Oniscidea)

Almerão, Maurício Pereira; Fagundes, Nelson J. R.; Araujo, Paula Beatriz; Verne, Sébastien; Grandjean, Frédéric; Bouchon, Didier; Araújo, Aldo Mellender de‏

doi:10.1590/s1415-47572012000600013

Cited by 11 publications

(7 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, among the 21 complete genomes, 15 are symbionts of insects belonging to either supergroup A or B (including 7 strains from species of Drosophila), 2 are symbionts of insects belonging to other supergroups (F and E) and 4 are symbionts of nematodes (supergroups L, C or D). Wolbachia has been identified in other arthropods, such as isopods [28,29] and arachnids [24]. Limited genomic data for isopods are available, for example a draft genome of wCon, infecting Cylisticus convexus and a draft genome of wVulC infecting Armadillidium vulgare [30].…”

Section: Introductionmentioning

confidence: 99%

Pseudoscorpion Wolbachia symbionts: diversity and evidence for a new supergroup S

et al. 2020

View full text Add to dashboard Cite

Background: Wolbachia are the most widely spread endosymbiotic bacteria, present in a wide variety of insects and two families of nematodes. As of now, however, relatively little genomic data has been available. The Wolbachia symbiont can be parasitic, as described for many arthropod systems, an obligate mutualist, as in filarial nematodes or a combination of both in some organisms. They are currently classified into 16 monophyletic lineage groups ("supergroups"). Although the nature of these symbioses remains largely unknown, expanded Wolbachia genomic data will contribute to understanding their diverse symbiotic mechanisms and evolution. Results: This report focuses on Wolbachia infections in three pseudoscorpion species infected by two distinct groups of Wolbachia strains, based upon multi-locus phylogenies. Geogarypus minor harbours wGmin and Chthonius ischnocheles harbours wCisc, both closely related to supergroup H, while Atemnus politus harbours wApol, a member of a novel supergroup S along with Wolbachia from the pseudoscorpion Cordylochernes scorpioides (wCsco). Wolbachia supergroup S is most closely related to Wolbachia supergroups C and F. Using target enrichment by hybridization with Wolbachia-specific biotinylated probes to capture large fragments of Wolbachia DNA, we produced two draft genomes of wApol. Annotation of wApol highlights presence of a biotin operon, which is incomplete in many sequenced Wolbachia genomes. Conclusions: The present study highlights at least two symbiont acquisition events among pseudoscorpion species. Phylogenomic analysis indicates that the Wolbachia from Atemnus politus (wApol), forms a separate supergroup ("S") with the Wolbachia from Cordylochernes scorpioides (wCsco). Interestingly, the biotin operon, present in wApol, appears to have been horizontally transferred multiple times along Wolbachia evolutionary history.

show abstract

Section: Introductionmentioning

confidence: 99%

Pseudoscorpion Wolbachia symbionts: diversity and evidence for a new supergroup S

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In Brazil, there are some reports regarding the detection of Wolbachia in limited, specific arthropod groups, but no general surveys of Wolbachia distribution among arthropods have so far been conducted. For example, infection of Wolbachia has been detected in two species of Balloniscus (Crustacea, Oniscidea) (Almerão et al ., 2012) and in some species of Diptera in Culicidae (de Albuquerque et al ., 2011; de Almeida et al ., 2011; Morais et al ., 2012; Baton et al ., 2013) and in Hymenoptera (Formicidae) (Martins et al ., 2012). Here, we show the incidence of Wolbachia in different insect orders from the northern and southeastern regions of Brazil using three different markers ( 16S rRNA, wsp and ftsZ ), and the observed incidence corroborates the previously reported widespread nature of this bacterium.…”

Section: Introductionmentioning

confidence: 99%

Broader prevalence of Wolbachia in insects including potential human disease vectors

Oliveira¹,

Gonçalves²,

Baton³

et al. 2015

Bull. Entomol. Res.

View full text Add to dashboard Cite

Wolbachia are intracellular, maternally transmitted bacteria considered the most abundant endosymbionts found in arthropods. They reproductively manipulate their host in order to increase their chances of being transmitted to the offspring, and currently are being used as a tool to control vector-borne diseases. Studies on distribution of Wolbachia among its arthropod hosts are important both for better understanding why this bacterium is so common, as well as for its potential use as a biological control agent. Here, we studied the incidence of Wolbachia in a broad range of insect species, collected from different regions of Brazil, using three genetic markers (16S rRNA, wsp and ftsZ), which varied in terms of their sensitivity to detect this bacterium. The overall incidence of Wolbachia among species belonging to 58 families and 14 orders was 61.9%. The most common positive insect orders were Coleoptera, Diptera, Hemiptera and Hymenoptera, with Diptera and Hemiptera having the highest numbers of Wolbachia-positive families. They included potential human disease vectors whose infection status has never been reported before. Our study further shows the importance of using quantitative polymerase chain reaction for high-throughput and sensitive Wolbachia screening.

show abstract

“…The pure diet of TM, BLP, CP, or a combination of these has been reported to be effective for Anopheles larval development, but dependent on the amount per larva per day [ 23 , 24 ] . The low overall larval survival rates observed (including controls), may have been due to the low quantity of diet per larva per day [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…All treatments, control included, were found to have similar or better effects on most of the assessed parameters including larval survival rates, duration of immature stages, egg production and adult body size. As fecundity and mating capabilities are known to rely on adult body size [ 15 , 17 – 19 , 28 ] the similarity in the body size correlates with the lack of difference observed in the egg production and indicates a probable similarity in male mating capabilities. However, since the success of SIT requires high quality of produced males in terms of mating competitiveness, further study should be conducted to evaluate this parameter on mass-reared males using the different promising larval diets to definitely figure out the most suitable diet mixture.…”

Section: Discussionmentioning

confidence: 99%

Cost-effective larval diet mixtures for mass rearing of Anopheles arabiensis Patton (Diptera: Culicidae)

et al. 2017

View full text Add to dashboard Cite

BackgroundLarval nutrition, particularly diet quality, is a key driver in providing sufficient numbers of high quality mosquitoes for biological control strategies such as the sterile insect technique. The diet currently available to mass rear Anopheles arabiensis, referred here to as the “IAEA diet”, is facing high costs and difficulties concerning the availability of the bovine liver powder component. To promote more affordable and sustainable mosquito production, the present study aimed to find alternative diet mixtures. Eight cheaper diet mixtures comprised of varying proportions of tuna meal (TM), bovine liver powder (BLP), brewer’s yeast (BY), and chickpea (CP) were developed and evaluated through a step by step assessment on An. arabiensis larvae and adult life history traits, in comparison to the IAEA diet which served as a basis and standard.ResultsFour mixtures were found to be effective regarding larval survival to pupation and to emergence, egg productivity, adult body size and longevity. These results suggest that these different diet mixtures have a similar nutritional value that support the optimal development of An. arabiensis larvae and enhance adult biological quality and production efficiency, and thus could be used for mass rearing.ConclusionsOur study demonstrated that four different diet mixtures, 40 to 92% cheaper than the IAEA diet, can result in a positive assessment of the mosquitoes’ life history traits, indicating that this mosquito species can be effectively mass reared with a significant reduction in costs. The mixture comprised of TM + BY + CP is the preferred choice as it does not include BLP and thus reduces the cost by 92% compared to the IAEA diet.

show abstract

First record of Wolbachia in South American terrestrial isopods: prevalence and diversity in two species of Balloniscus (Crustacea, Oniscidea)

Cited by 11 publications

References 61 publications

Pseudoscorpion Wolbachia symbionts: diversity and evidence for a new supergroup S

Pseudoscorpion Wolbachia symbionts: diversity and evidence for a new supergroup S

Broader prevalence of Wolbachia in insects including potential human disease vectors

Cost-effective larval diet mixtures for mass rearing of Anopheles arabiensis Patton (Diptera: Culicidae)

Contact Info

Product

Resources

About