Caracterização molecular de populações de Euseius citrifolius Denmark & Muma e Euseius concordis (Chant) (Acari: Phytoseiidae) utilizando o seqüenciamento das regiões ITS1 e ITS2

Abstract

Mites have usually been identified by their morphological characteristics. However, morphological evidences are not always sufficient to distinguish between closely related species, leading taxonomists to consider additional ecological, biological and, more recently, molecular characteristics in this process. The molecular characterization of populations of Euseius citrifolius Denmark & Muma and Euseius concordis (Chant) was done by sequencing the internal transcribed spacers ITS1 and ITS2 using the P1 (5'-AGAGGAAGTAAAAGTCGTAACAAG-3)' and P2 (5'-ATATGCTTAAATTCAGGGGG-3') primers, located in 18S and 28S regions of the ribosomal DNA. The following populations were characterizate: E. citrifolius: Arroio do Meio-RS, Campinas-SP and Petrolina-PE; E. concordis: Arroio do Meio, Jaguariúna-SP, Pontes e Lacerda-MT, Petrolina and Viçosa-MG. The sequencing of ITS1 and ITS2 allowed the discrimination between the group of populations of E. citrifolius from E. concordis. The similarity between the sequences was more than 94%. Most of the variation between populations was observed more in ITS1 than in ITS2. The sequencing of ITS region helps the identification of phytoseiid species when the populations show citoplasmatic incompatibility and need additional information.

Reproductive compatibility and genetic and morphometric variability among populations of the predatory mite, Amblyseius largoensis (Acari: Phytoseiidae), from Indian Ocean Islands and the Americas

“…The lack of oviposition by unmated females has been observed for the phytoseiid mites studied to date (e.g., see Croft, 1970;Moraes and McMurtry, 1981;Noronha et al, 2003). In this study, unmated females from both La Réunion and Roraima, Brazil populations did not oviposit during their entire life cycle, which demonstrated that crossing is a prerequisite for oviposition.…”

Morphological, molecular and cross-breeding analysis of geographic populations of coconut-mite associated predatory mites identified as Neoseiulus baraki: evidence for cryptic species?

“…Hence, molecular and other tools may be helpful for taxonomic identification ). These techniques have allowed researchers to look beyond morphological similarities by searching for genetic and other differences to answer taxonomic questions (Navajas et al 1996(Navajas et al , 1998Hinomoto et al 2001Hinomoto et al , 2007Jeyaprakash and Hoy 2002;Noronha et al 2003;Ramadan et al 2004;Klimov et al 2004;Tixier et al 2006aTixier et al , 2010Ros and Breeuwer 2010;Okassa et al 2009Okassa et al , 2010Okassa et al , 2011Kanouh et al 2010). Here, we address the question whether predatory mites collected from different geographic localities and previously identified as Neoseiulus baraki Athias-Henriot represent more than one closely related species.…”

Molecular and morphological boundaries of the predatory miteNeoseiulus californicus(McGregor) (Acari: Phytoseiidae)

“…Little is known on the reliability of characters used for Phytoseiidae species discrimination because intraspecific variations are not well defined. To assist diagnostics, molecular markers have been recently applied within Phytoseiidae (Jeyaprakash & Hoy, 2002;Noronha et al, 2003;Tixier et al, 2006aTixier et al, , b, 2008aTixier et al, , 2010Okassa et al, 2009Okassa et al, , 2010Kanouh et al, 2010). However, because morphological analyses require distinct characters, molecular analyses require the accurate characterization of intraspecific variations to discriminate species from populations of the same species (Wiens & Servedio, 2000;Dayrat, 2005;Meier, 2008;Cardoso, Serrano & Vogler, 2009).…”