Mycotoxicological and palynological profiles of commercial brands of dried bee pollen

Deveza, Michele Valadares; Keller, Kelly Moura; Lorenzon, Maria Cristina Affonso; Nunes, Lucila Maria Teixeira; Sales, Érika Oliveira; Barth, Ortrud Monika

doi:10.1590/s1517-838246420140316

Cited by 29 publications

(23 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…as compared to spring pollen. Cladosporium fungi are present in the environment on substrates of various chemical composition, their spores are often found in humid air (Deveza et al, 2015). In the studies, C. cladosporioides was one of the most frequently isolated micromycetes in pollen collected at the end of summer.…”

Section: Resultsmentioning

confidence: 99%

“…According to the structural components of pollen, there is no other product that could substitute them (Gendrolis, 2012). Pollen contains about 200 different substances (including proteins, carbohydrates, flavonoids, enzymes, trace elements, abundant amino acids and vitamins (Deveza et al, 2015;Kieliszek et al, 2018)). Bee pollen is delivered as a healthy food supplement and a dietary product (Hassan, 2011).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence of microscopic fungi in bee pollen

Sinkevičienė¹,

Amšiejus²

2019

biologija

View full text Add to dashboard Cite

One of the problems with storing bee products – pollen – is their microbiological pollution. With high levels of pollen contamination by microscopic fungi, toxins synthesized by the fungi of some genera can have a negative impact on human health. During the experiment, the prevalence of microscopic fungi in pollen was evaluated, and their genera and species were identified. Pollen samples were collected at different times of the year – spring and summer – in order to ascertain the abundance and diversity of different fungal genera and species. The dilution method (CFU/g) was used to determine the number of fungal strains per sample and their amount. The total number of fungal strains in the pollen collected in spring ranged from 1.3 to 5.7 × 10–3 CFU/g, in summer –1.0 to 5.8 × 10–3 CFU/g. In the pollen, 11 genera and six species of fungi were identified. The number of fungal genera and species in pollen collected in spring and summer varied insignificantly. In spring, ten genera and six species of fungi were isolated from pollen, and in summer 11 genera and six species were identified. Penicillium and Alternaria fungi dominated the bee pollen.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Prevalence of microscopic fungi in bee pollen

Sinkevičienė¹,

Amšiejus²

2019

biologija

View full text Add to dashboard Cite

show abstract

“…Thus are the high hygienic standards and proper handling of the product in every stage of production crucial for its quality and safety. Opposite activities can cause the product becoming undesirable and harmful for human health (Deveza et al, 2015;Beev et al, 2018;Kieliszek et al, 2018). In this investigation we examined i) the microbiological quality of bee pollen by determining the total aerobic count, yeast and mold count, and coliform bacteria, ii) the total phenolic content, ii) antioxidative potential (AOP), and iii) antimicrobial activity of bee pollen gathered from 7 different Slovenian statistical (geographical) regions.…”

Section: Introductionmentioning

confidence: 99%

Microbiological Quality, Antioxidative and Antimicrobial Properties of Slovenian Bee Pollen

Šimunović¹,

Abramovič²,

Lilek³

et al. 2019

AGR

View full text Add to dashboard Cite

Bee pollen can be considered as perfect food with a great nutritional value, highprotein and essential amino acid content, vitamins and minerals. It can be a sourceof healthy nutrients, but as an animal product also of harmful microbialcontaminants. The aim of our study was to determine potential health risks andbenefits of Slovenian bee pollen. We determined its i) microbiological burden:aerobic mesophilic microorganisms, yeast, molds, and coliform bacteria in CFU/g;ii) polyphenolic content: the Folin-Ciocalteu method (mgGA/g); iii) antioxidativepotential (AOP): DPPH• scavenging assay (EC50 in mgGA/L); and iv)antimicrobial activity (MIC): microdilution method on Escherichia coli, Listeriamonocytogenes and Campylobacter jejuni. We analyzed 14 samples of bee pollengathered from 7 Slovenian geographical regions, from April until May 2017. Themicrobiological burden was high, with all indicator tests reaching up to 6.78log10CFU/g of bee pollen, but the number of coliform bacteria in all samples from2.00 to 4.48 log10CFU/g. The polyphenolic content and AOP of the samples wasgood, with up to 13.1 mg GA/g and as low as 2.4 mgGA/L (EC50), respectively.Interestingly, antimicrobial activity was not always in correlation withpolyphenolic content, but always strongly against E. coli, substantial against C.jejuni, and negligible against L. monocytogenes. Our results show a great healthpotential of bee pollen for human health, but also the need of bee pollen processingimprovement for its standardized quality and safety.

show abstract

“…Bee pollen is considered to be one of the most complete food items in nature (Petrovic et al, 2014). Pollen contains about 200 different substances, including proteins, carbohydrates, flavonoids, enzymes, trace elements, and numerous amino acids and vitamins (Deveza et al, 2015;Kieliszek et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…An important issue concerning the criteria of pollen quality is its purity and microbiological safety (Deveza et al, 2015). It seems that the most critical step is the removal of pollen from traps.…”

Section: Introductionmentioning

confidence: 99%

Mycobiota in bee pollen collected by different types of traps

Sinkevičienė

Amšiejus

2019

Zemdirbyste-Agriculture

View full text Add to dashboard Cite

The aim of this study was to determine the impact of different types of bee pollen traps on the microbiological contamination of pollen influenced by the duration of pollen retention in traps. A total of 96 bee pollen samples were analyzed. The total count of microorganisms in the bee pollen during the study period varied from 4.5 to 19.3 × 10 3 cfu g-1. Moisture content in the pollen was optimal for the growth and proliferation of microorganisms. In the pollen, the following most common 7 genera of fungi were found: Penicillium, Fusarium, Alternaria, Acremonium, Cladosporium, Mucor and Rhizopus. A study of the mycobiota of pollen collected from four different traps showed that the total number of microorganisms varied depending on the type of trap and pollen retention period. The pollen collected after 9 hours retention in traps was less contaminated with the microorganisms than that collected after 24 and 48 hours. The count of mycobiota was the lowest (4.5 × 10 3 cfu g-1) in the pollen sampled after 9 hours retention from high-bottom traps compared to other types of traps. The highest contamination (19.3 × 10 3 cfu g-1) of fresh bee pollen was found after 48 hours in traps with thermo chambers. Fungal contamination was the higest in the bee pollen collected by traps mounted at hive entrance after 9 and 48 hours, respectively 124 and 150 units. The Penicillium fungi dominated the pollen in traps with thermo chambers, Fusarium-in low-bottom, Alternaria-in the pollen of traps mounted at beehive entrance.

show abstract

Mycotoxicological and palynological profiles of commercial brands of dried bee pollen

Cited by 29 publications

References 17 publications

Prevalence of microscopic fungi in bee pollen

Prevalence of microscopic fungi in bee pollen

Microbiological Quality, Antioxidative and Antimicrobial Properties of Slovenian Bee Pollen

Mycobiota in bee pollen collected by different types of traps

Contact Info

Product

Resources

About