Draft genome sequence of bla&lt;sub&gt;Veb-1&lt;/sub&gt;, bla&lt;sub&gt;oxa-10&lt;/sub&gt;producing multi-drug resistant (MDR) &lt;italic&gt;Pseudomonas aeruginosa&lt;/italic&gt;strain VRFPA09 recovered from bloodstream infection

Murugan, Nandagopal; Malathi, Jambulingam; Vetrivel, Umashankar; Hn, Madhavan

doi:10.1590/s1517-838246320150154

Cited by 3 publications

(5 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Whole-genome sequencing would be necessary to decipher the overall potential of antimicrobial resistance genes, multiple drug resistance and virulence-factor-encoding genes, as has recently been shown for one of the P. aeruginosa strains causing BSI [ 41 ]. The genome sequence of a recently identified and highly virulent P. aeruginosa PA45 strain was also provided by other researchers.…”

Section: Discussionmentioning

confidence: 99%

Decoding Genetic Features and Antimicrobial Susceptibility of Pseudomonas aeruginosa Strains Isolated from Bloodstream Infections

Bogiel¹,

Depka²,

Rzepka³

et al. 2022

IJMS

View full text Add to dashboard Cite

Pseudomonas aeruginosa is a Gram-negative rod and an etiological factor of opportunistic infections. The infections of this etiology appear mostly among hospitalized patients and are relatively hard to treat due to widespread antimicrobial resistance. Many virulence factors are involved in the pathogenesis of P. aeruginosa infection, the coexistence of which have a significant impact on the course of an infection with a particular localization. The aim of this study was to assess the antimicrobial susceptibility profiles and the frequency of genes encoding selected virulence factors in clinical P. aeruginosa strains isolated from bloodstream infections (BSIs). The following genes encoding virulence factors of enzymatic activity were assessed: lasB, plC H, plC N, nan1, nan2, aprA and phzM. The frequency of the genes encoding the type III secretion system effector proteins (exoU and exoS) and the genes encoding pilin structural subunits (pilA and pilB) were also investigated. The occurrence of virulence-factor genes was assessed using polymerase chain reactions, each in a separate reaction. Seventy-one P. aeruginosa strains, isolated from blood samples of patients with confirmed bacteremia hospitalized at the University Hospital No. 1 of Dr. Antoni Jurasz in Bydgoszcz, Poland, were included in the study. All the investigated strains were susceptible to colistin, while the majority of the strains presented resistance to ticarcillin/clavulanate (71.8%), piperacillin (60.6 %), imipenem (57.7%) and piperacillin/tazobactam (52.1%). The presence of the lasB and plC H genes was noted in all the tested strains, while the plC N, nan2, aprA, phzM and nan1 genes were identified in 68 (95.8%), 66 (93.0%), 63 (88.7%), 55 (77.5%) and 34 (47.9%) isolates, respectively. In 44 (62.0%) and 41 (57.7%) strains, the presence of the exoU and exoS genes was confirmed, while the pilA and pilB genes were noted only in 14 (19.7%) and 3 (4.2%) isolates, respectively. This may be due to the diverse roles of these proteins in the development and maintenance of BSIs. Statistically significant correlations were observed between particular gene pairs’ coexistence (e.g., alkaline protease and neuraminidase 2). Altogether, twenty-seven distinctive genotypes were observed among the studied strains, indicating the vast variety of genetic compositions of P. aeruginosa strains causing BSIs.

show abstract

Section: Discussionmentioning

confidence: 99%

Decoding Genetic Features and Antimicrobial Susceptibility of Pseudomonas aeruginosa Strains Isolated from Bloodstream Infections

Bogiel¹,

Depka²,

Rzepka³

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…We designed primers for the selected genes found to be most prevalent among clinical studies as well as our own observation from NGS based study and other reports [12–21]. Multiplex PCR Primer designed against drug resistance gene include Group‐1; (ESBL‐MBL) blaTem, blaOXA, blaCTX‐M‐15, blaVim) Group‐2, (blaGes, blaVeb and DIM and AmpC) Efflux pump genes‐ Group‐3 (MexA,B‐oprM); Group‐4 (MexC,D‐oprJ); Group‐5 (Mex X,Y‐oprN) Group‐6 (oprD, nfxB, MexR) primers were purchased from Eurofins Genomics India Pvt Ltd, Bangalore, India.…”

Section: Methodsmentioning

confidence: 99%

“…From our experience, emergence of culture negative intraocular specimens is steadily increasing, which leads to unwanted avoidable blindness. Hence, the current study designed to target most common betalactamase and efflux pump genes among P aeruginosa reported in literature and our own study experiences [3–5,12–17]. In addition, the current study will assess the coexistence of ESBL, MBL and efflux pump genes among P. aeruginosa isolates obtained from variety of clinical specimens (ocular tissues, blood, urine, pus and etc) using multiplex PCR and the same were compared with Phenotypic methods [18].…”

Section: Introductionmentioning

confidence: 99%

Application of six multiplex PCR's among 200 clinical isolates of Pseudomonas aeruginosa for the detection of 20 drug resistance encoding genes

Murugan

Malathi

Therese

et al. 2017

The Kaohsiung J of Med Scie

Self Cite

View full text Add to dashboard Cite

Pseudomonas aeruginosa (P. aeruginosa) is a menacing opportunistic, nosocomial pathogen; become a growing concern as conventional antimicrobial therapy is now futile against it. Multi‐drug resistant P. aeruginosa (MDRPA) has distinctive resistance mechanisms such as production of β‐lactamases, repression of porin genes and over‐expression of efflux pumps. The focus of this study is to standardize and application of multiplex PCR (mPCR) to detect the presence of betalactamase genes encoding blaTem, blaOXA, blaCTX‐M‐15, blaVim, blaGes, blaVeb, blaDIM, AmpC and Efflux pump genes encoding Mex A,B‐oprM, Mex C,D‐oprJ, Mex X,Y‐oprN, oprD, nfxB, MexR. A total of 200 clinical isolates of P. aeruginosa were tested for the presence of the above mentioned genes genotypically through mPCR and characterized by phenotypic methods for ESBL and MBL production. Out of 200 isolates, 163 (81.5%) nfxB regulator gene, 102 (51%) MexA, 96 (48%) MexC, 93 (46.5%) MexB, 86 (43%) MexD, 81 (40.5%) OprM, 74 (37%) OprJ, 72 (36%) OprD and MexR, 53 (26.5%) Mex X and OprN, 49 (24.5%) MexY gene. Betalactamase genes 145 (72.5%) blaTem, 67 (33.5%) blaOXA, 35 (17.5%) blaVim, 25(12.50%), 23 (11.50%) blaVeb, 21 (11.5%) blaGes, 14 (7%) Ctx‐m and 10 (5%) AmpC and 5 (2.5%) blaDim‐1 gene were tested positive by mPCR. Phenotypically 38 (19%) and 29 (14.5%) out of 200 tested positive for ESBL and MBL production. Application of this mPCR on clinical specimens is fast, accurate, specific and low‐cost reliable tool for the screening, where culture negative Eubacterial PCR positive cases for an early molecular detection of drug resistance mechanism assisting the clinician to treat the disease with appropriate antibiotic selection.

show abstract

“…Further the organism was genotypically confirmed up to species level using 16s ribosomal RNA gene based sequencing result against blast tool available at NCBI database revealed 99% homology to all the existing P . aeruginosa strains in the database inclusive of our previously reported strains VRFPA01-VRFPA09 [5] , [6] , [7] , [8] , [9] , [10] , hence the strain isolated from scleritis was designated as P . aeruginosa VRFPA10.…”

Section: Methodsmentioning

confidence: 99%

“…Whole genome study was undertaken by utilizing Ion Torrent (PGM) sequencer with 400-bp read chemistry (Life Technologies) accordance with manufacturer's instructions. In brief, genomic DNA from VRFPA10 was isolated from the overnight cultures with DNeasy miniprep kit (Qiagen, Hilden, Germany) and the sequencing protocol was followed as per previous study [5] , [6] , [7] , [8] , [9] , [10] .…”

Section: Methodsmentioning

confidence: 99%

Virulence genome analysis of Pseudomonas aeruginosa VRFPA10 recovered from patient with scleritis

et al. 2017

Self Cite

View full text Add to dashboard Cite

Infectious keratitis is a major cause of blindness, next to cataract and majority of cases are mainly caused by gram negative bacterium Pseudomonas aeruginosa (P. aeruginosa). In this study, we investigated a P. aeruginosa VRFPA10 genome which exhibited susceptibility to commonly used drugs in vitro but the patient had poor prognosis due to its hyper virulent nature. Genomic analysis of VRFPA10 deciphered multiple virulence factors and P.aeruginosa Genomic Islands (PAGIs) VRFPA10 genome which correlated with hyper virulence nature of the organism. The genome sequence has been deposited in DDBJ/EMBL/GenBank under the accession numbers LFMZ01000001-LFMZ01000044.

show abstract

Draft genome sequence of bla<sub>Veb-1</sub>, bla<sub>oxa-10</sub>producing multi-drug resistant (MDR) <italic>Pseudomonas aeruginosa</italic>strain VRFPA09 recovered from bloodstream infection

Cited by 3 publications

References 8 publications

Decoding Genetic Features and Antimicrobial Susceptibility of Pseudomonas aeruginosa Strains Isolated from Bloodstream Infections

Decoding Genetic Features and Antimicrobial Susceptibility of Pseudomonas aeruginosa Strains Isolated from Bloodstream Infections

Application of six multiplex PCR's among 200 clinical isolates of Pseudomonas aeruginosa for the detection of 20 drug resistance encoding genes

Virulence genome analysis of Pseudomonas aeruginosa VRFPA10 recovered from patient with scleritis

Contact Info

Product

Resources

About