Halotolerant bacteria in the São Paulo Zoo composting process and their hydrolases and bioproducts

Oliveira, Lilian Caroline Gonçalves de; Ramos, Patricia; Marem, Alyne; Kondo, Márcia Y.; Rocha, Rafael Costa Santos; Bertolini, Thiago; Silveira, Marghuel Aparecida Vieira; Cruz, João Batista da; Vasconcellos, Suzan Pantaroto de; Juliano, Luiz; Okamoto, Débora N.

doi:10.1590/s1517-838246220130316

Cited by 12 publications

(8 citation statements)

References 38 publications

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“…Isolates from effluents of textile industries also showed the ability to decolorize the utilized azo dyes [ 11 ]. Halotolerant bacteria, recovered from the composting process, were able to produce hydrolases, lipases, proteases, amylases, cellulases and biopolymers [ 12 ]. Four halotolerant species ( Bacillus atrophaeus, Halomonas shengliensis, Halomonas koreensis and Virgibacillus salarius ) showed the ability to metabolize hydrocarbons and isolates as V. salarius and Brevibacillus sp.…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of halotolerant soil bacteria from coastal Patenga area

2017

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ObjectiveHalotolerant bacteria have multiple uses viz. fermentation with lesser sterility control and industrial production of bioplastics. Moreover, it may increase the crop productivity of coastal saline lands in Bangladesh by transferring the salt tolerant genes into the plants. The study focused on the isolation and identification of the halotolerant bacteria from three soil samples, collected from coastal Patenga area. The samples were inoculated in nutrient media containing a wide range of salt concentrations.ResultsAll the samples showed 2, 4 and 6% (w/v) salt tolerance. The isolates from Patenga soil (4, 6%) and beach soil (2%) showed catalase activity and all the isolates showed negative results for oxidase activity, indole production, lactose and motility. All the samples provided positive results for dextrose fermentation. Other tests provided mixed results. Based on the morphological characteristics, biochemical tests and ABIS software analysis the isolates fall within the Enterobacteriaceae, Clostridium and Corynebacterium, with a predominance of Vibrios. Overall the isolates can be considered as mild halotolerant, with the best growth observed at lower salinities and no halophilism detected. Among many possibilities, the genes responsible for the salt tolerant trait in these species can be identified, extracted and inserted into the crop plants to form a transgenic plant to result in higher yield for the rest of the year.

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Section: Introductionmentioning

confidence: 99%

Isolation and identification of halotolerant soil bacteria from coastal Patenga area

2017

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“…Some of the metagenome-derived halotolerant enzymes also originated from saline environments, such as marine water [ 27 , 37 , 41 , 79 ], deep sea sponges [ 42 , 80 ], deep-sea sediments [ 25 , 81 ], and deep-sea shrimps [ 82 ]. Recently, compost was reported as an habitat for halophilic and halotolerant microorganisms [ 83 , 84 ]. To our knowledge, Est56 is the first halotolerant esterase identified from a compost metagenome.…”

Section: Discussionmentioning

confidence: 99%

A Novel Carboxylesterase Derived from a Compost Metagenome Exhibiting High Stability and Activity towards High Salinity

Daniel

2021

Genes

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Halotolerant lipolytic enzymes have gained growing interest, due to potential applications under harsh conditions, such as hypersalinity and presence of organic solvents. In this study, a lipolytic gene, est56, encoding 287 amino acids was identified by functional screening of a compost metagenome. Subsequently, the gene was heterologously expressed, and the recombinant protein (Est56) was purified and characterized. Est56 is a mesophilic (Topt 50 °C) and moderate alkaliphilic (pHopt 8) enzyme, showing high thermostability at 30 and 40 °C. Strikingly, Est56 is halotolerant as it exhibited high activity and stability in the presence of up to 4 M NaCl or KCl. Est56 also displayed enhanced stability against high temperatures (50 and 60 °C) and urea (2, 4, and 6 M) in the presence of NaCl. In addition, the recently reported halotolerant lipolytic enzymes were summarized. Phylogenetic analysis grouped these enzymes into 13 lipolytic protein families. The majority (45%) including Est56 belonged to family IV. To explore the haloadaptation of halotolerant enzymes, the amino acid composition between halotolerant and halophilic enzymes was statistically compared. The most distinctive feature of halophilic from non-halophilic enzymes are the higher content of acidic residues (Asp and Glu), and a lower content of lysine, aliphatic hydrophobic (Leu, Met and Ile) and polar (Asn) residues. The amino acid composition and 3-D structure analysis suggested that the high content of acidic residues (Asp and Glu, 12.2%) and low content of lysine residues (0.7%), as well as the excess of surface-exposed acidic residues might be responsible for the haloadaptation of Est56.

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“…In recent years, there has been growing interest in finding microbial lipases, principally from bacteria and fungi ( Table 2 ) [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] . Interestingly, these microorganisms are very attractive as biocatalysts due to their unique properties of adapting to extreme environmental conditions such as hypersaline habitats, high pressure, and extreme temperature.…”

Section: Novel Lipases Found In the Environmental Samplesmentioning

confidence: 99%

Marine Metagenome as a Resource for Novel Enzymes

Alma’abadi

Gojobori

Mineta

2015

Genomics, Proteomics &Amp; Bioinformatics

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More than 99% of identified prokaryotes, including many from the marine environment, cannot be cultured in the laboratory. This lack of capability restricts our knowledge of microbial genetics and community ecology. Metagenomics, the culture-independent cloning of environmental DNAs that are isolated directly from an environmental sample, has already provided a wealth of information about the uncultured microbial world. It has also facilitated the discovery of novel biocatalysts by allowing researchers to probe directly into a huge diversity of enzymes within natural microbial communities. Recent advances in these studies have led to a great interest in recruiting microbial enzymes for the development of environmentally-friendly industry. Although the metagenomics approach has many limitations, it is expected to provide not only scientific insights but also economic benefits, especially in industry. This review highlights the importance of metagenomics in mining microbial lipases, as an example, by using high-throughput techniques. In addition, we discuss challenges in the metagenomics as an important part of bioinformatics analysis in big data.

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Halotolerant bacteria in the São Paulo Zoo composting process and their hydrolases and bioproducts

Cited by 12 publications

References 38 publications

Isolation and identification of halotolerant soil bacteria from coastal Patenga area

Isolation and identification of halotolerant soil bacteria from coastal Patenga area

A Novel Carboxylesterase Derived from a Compost Metagenome Exhibiting High Stability and Activity towards High Salinity

Marine Metagenome as a Resource for Novel Enzymes

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