Production and characterization of an extracellular lipase from Candida guilliermondii

Abstract

Extracellular lipases from the endophytic yeast Candida guilliermondii isolated from castor leaves (Ricinus communis L.) were produced using low-cost raw materials such as agro-industrial residues and applying them in the esterification of oleic acid for evaluating their potential use in biodiesel production. After partial purification using ammonium sulfate, the enzyme was characterized and presented higher activity (26.8 ± 1.5 U mL−1) in the presence of 5 mmol L−1 NaCl at 30 °C and pH 6.5. The production through submerged fermentation was formerly performed in 150 mL erlenmeyer flasks and, once the enzyme production was verified, assays in a 14 L bioreactor were conducted, obtaining 18 ± 1.4 U mL−1. The produced enzyme was applied in the oleic acid esterification under different solvents: hexane, cyclohexane or cyclohexanone) and different acid:alcohol molar ratios. Higher ester conversion rate (81%) was obtained using hexane and the molar ratio of 1:9 was the best conditions using methanol. The results suggest the potential for development of endophytic yeast in the production of biocatalyst through submerged fermentation using agroindustrial residues as culture medium.

Molecular, physiological, and biochemical characterization of extracellular lipase production by Aspergillus niger using submerged fermentation

“…SmF is the most widely used method to produce industrial enzymes by more than 75%. This method has several advantages over solid-state fermentation culture (SSF), such as easy and large-scale procurement of the lipase and controlling the factors that affect the production (Sundar & Kumaresapillai, 2013;Oliveira, Fernandes & Mariano, 2014). Eleven Aspergillus isolates were tested for their ability of producing lipase using SmF as a growth medium to obtain lipase in culture.…”

“…Absorbance was measured in spectrophotometer (SPECTRO UV-VIS DOUBL) at 410 nm and the unit of the enzymatic activity (U) was determined from the release of 1 µM of pNPP per minute. A standard curve was used to measure enzyme activity, as described previously by Oliveira, Fernandes & Mariano (2014).…”

Microbial lipases and their industrial applications: a comprehensive review

“…The short-chain alcohol tolerant abilities of lipase and higher thermostability form very suitable for usage in the production of biodiesel. Candida antarctica lipase in immobilized form catalyzed methanolysis of soybean oil for the production of biodiesel [ 644 , 645 ].…”

Produção de lipase por leveduras isoladas de frutos de palmeiras

“…A obtenção de lipases pode ser feita a partir de vários organismos, entretanto, as microbianas são mais promissoras, sendo as leveduras usualmente utilizadas na produção dessas enzimas, pois são microrganismos encontrados em diferentes habitas, sendo assim uma valiosa fonte natural para identificação de novas fontes de lipases que podem favorecer novas propriedades catalíticas (Salihu et al, 2016;Oliveira et al, 2014). Os principais gêneros de leveduras produtoras de lipases são Candida, Yarrowia, Pichia, Rhodotorula e Saccharomycopsis (Liu & Kokare, 2017).…”