Quick stimulation of Alcanivorax sp. by bioemulsificant EPS2003 on microcosm oil spill simulation

Cappello, Simone; Genovese, Maria Inês; Denaro, Renata; Santisi, Santina; Volta, Anna; Bonsignore, Martina; Mancini, Giuseppe; Giuliano, Laura; Genovese, Luigi; Yakimov, Michail M.

doi:10.1590/s1517-83822014000400023

Cited by 12 publications

(4 citation statements)

References 24 publications

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“…Petroleum consists of a complex mixture of diverse aliphatic and aromatic hydrocarbons, resins, and asphaltenes [1], which affects crude oil's susceptibility to biodegradation and their environmental fate [2]. The high hydrophobicity and low solubility of hydrocarbon pollutants contribute to their accumulation in the marine sediments [3,4]. Oil contamination may have a greater impact on more sensitive environments, such as cold marine areas, where the harsh conditions lower the rate of natural attenuation, allowing the petroleum pollutants to persist more than 20 years in marine soil, permafrost, and deep-sea waters [5,6], raising problematic environmental concerns.…”

Section: Introductionmentioning

confidence: 99%

Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean)

et al. 2021

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Deep-sea sediments (DSS) are one of the largest biotopes on Earth and host a surprisingly diverse microbial community. The harsh conditions of this cold environment lower the rate of natural attenuation, allowing the petroleum pollutants to persist for a long time in deep marine sediments raising problematic environmental concerns. The present work aims to contribute to the study of DSS microbial resources as biotechnological tools for bioremediation of petroleum hydrocarbon polluted environments. Four deep-sea sediment samples were collected in the Mid-Atlantic Ridge, south of the Azores (North Atlantic Ocean). Their autochthonous microbial diversity was investigated by 16S rRNA metabarcoding analysis. In addition, a total of 26 deep-sea bacteria strains with the ability to utilize crude oil as their sole carbon and energy source were isolated from the DSS samples. Eight of them were selected for a novel hydrocarbonoclastic-bacterial consortium and their potential to degrade petroleum hydrocarbons was tested in a bioremediation experiment. Bioaugmentation treatments (with inoculum pre-grown either in sodium acetate or petroleum) showed an increase in degradation of the hydrocarbons comparatively to natural attenuation. Our results provide new insights into deep-ocean oil spill bioremediation by applying DSS hydrocarbon-degrading consortium in lab-scale microcosm to simulate an oil spill in natural seawater.

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Section: Introductionmentioning

confidence: 99%

Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean)

et al. 2021

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“…This positions EPS as an effective emulsifier, and it allows the macromolecule to interface the solubilization of hydrocarbons to enhance the biodegradation of oil-spill zones (Poli et al, 2010;Llamas et al, 2012;de Jesús Paniagua-Michel et al, 2014). Several studies now demonstrate that halophilic EPSs are competitive candidates for in situ degradation of long-chain n-alkanes and petroleum hydrocarbons (Cappello et al, 2012(Cappello et al, , 2014Gutierrez et al, 2013;Fan et al, 2020).…”

Section: Biotechnological Advantages Of Extremophilic Eps Over Traditional Biopolymersmentioning

confidence: 99%

Extremophilic Exopolysaccharides: Biotechnologies and Wastewater Remediation

et al. 2021

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Various microorganisms thrive under extreme environments, like hot springs, hydrothermal vents, deep marine ecosystems, hyperacid lakes, acid mine drainage, high UV exposure, and more. To survive against the deleterious effect of these extreme circumstances, they form a network of biofilm where exopolysaccharides (EPSs) comprise a substantial part. The EPSs are often polyanionic due to different functional groups in their structural backbone, including uronic acids, sulfated units, and phosphate groups. Altogether, these chemical groups provide EPSs with a negative charge allowing them to (a) act as ligands toward dissolved cations as well as trace, and toxic metals; (b) be tolerant to the presence of salts, surfactants, and alpha-hydroxyl acids; and (c) interface the solubilization of hydrocarbons. Owing to their unique structural and functional characteristics, EPSs are anticipated to be utilized industrially to remediation of metals, crude oil, and hydrocarbons from contaminated wastewaters, mines, and oil spills. The biotechnological advantages of extremophilic EPSs are more diverse than traditional biopolymers. The present review aims at discussing the mechanisms and strategies for using EPSs from extremophiles in industries and environment bioremediation. Additionally, the potential of EPSs as fascinating biomaterials to mediate biogenic nanoparticles synthesis and treat multicomponent water contaminants is discussed.

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“…Integrating traditional analytic techniques with a simultaneous biological investigation could provide an early warning of the potential effects (positive or negative) of an adopted technological solution [25][26][27] before its implementation.…”

Section: Introductionmentioning

confidence: 99%

Assessing the Impact of Hg-Contaminated Sediments Washing through Sentinel Species: A Mesocosm Approach

Mancini,

Cappello,

De Marco

et al. 2023

Water

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This study combines a traditional chemical characterization with a simultaneous biological evaluation through histological, immunohistochemical, and enzymatic observations to assess the efficiency and sustainability of soil washing on Hg-contaminated sediment in terms of the bioavailability of the contaminant before and after the treatment, as well as the potential drawbacks of the treatment that are not revealed by a simple chemical characterization of treated sediments on its own. Different extracting agents, that is, ethylenedinitrilotetraacetic acid (EDTA), ethylenediaminedisuccinic acid (EDDS), sodium thiosulfate, potassium iodide (KI), and iodine (I2), have been compared in this work to evaluate their efficiency in the removal of Hg from contaminated sediments. Speciation analysis was applied to assess the mobility of Hg from different fractions of aged sediments. Biological evaluation was carried out through the use of large mesocosms and Mytilus galloprovincialis as biological sentinels. Results from bench scale tests have shown Hg removal of up to 93% by means of the multi-step KI/I2 washing process of the sediment. Results from histological, immunohistochemical, and enzymatic analysis have shown significant differences in the degree of alteration of biological tissues and their functional integrity between organisms in contact with contaminated and restored sediments. The reduction in 5-HT3R immunopositivity in the mesocosm with treated sediments suggests a tendency for mussels to recover a healthy condition. This result was also confirmed by the measurement of the enzymatic activity of AChE in mussel gills, which was significantly reduced in organisms from the mesocosm with polluted sediments compared with those from the one with restored sediments. The proposed approach could help stakeholders all over the world select, at an early stage, the most efficient cleaning action from a more holistic perspective, including not only pollutant concentration and economic reduction but also a direct assessment of the ultimate impact of the selected process on the biological system.

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Quick stimulation of Alcanivorax sp. by bioemulsificant EPS2003 on microcosm oil spill simulation

Cited by 12 publications

References 24 publications

Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean)

Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean)

Extremophilic Exopolysaccharides: Biotechnologies and Wastewater Remediation

Assessing the Impact of Hg-Contaminated Sediments Washing through Sentinel Species: A Mesocosm Approach

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