Screening of wild type Streptomyces isolates able to overproduce clavulanic acid

Marques, Daniela de Araújo Viana; Santos-Ebinuma, Valéria de Carvalho; Oliveira, Patrícia Maria Sobral de; Lima, Gláucia Manoella de Souza; Araújo, Janete Magalí de; Lima-Filho, José Luiz de; Converti, Attílio; Pessoa, Adalberto; Porto, Ana Lúcia Figueiredo

doi:10.1590/s1517-83822014000300022

Cited by 6 publications

(5 citation statements)

References 35 publications

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“…Those were oleandomycin, 2-piperidinone and derivatives of either erythromycin or tylonolide. S. variabilis is reported for the production of L-Glutaminase, imunosuppresive, clavulanic acid, glycoside antibiotic and 1-hydroxy-1-norresistomycin (Abd-Alla et al, 2013, 2016; Marques et al, 2014; Ramalingam and Rajaram, 2016). …”

Section: Discussionmentioning

confidence: 99%

Isolation, Screening, and Identification of Novel Isolates of Actinomycetes from India for Antimicrobial Applications

et al. 2016

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The search for novel bioactive compounds from the natural environment has rapidly been gaining momentum with the increase in multi-drug resistant (MDR) pathogens. In the present study, the antimicrobial potential of novel actinomycetes has been evaluated by initial screening of six soil samples. Primary and secondary screening was performed against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Candida albicans, Candida tropicalis, Trichophyton rubrum, and other MDR bacterial and fungal test strains, thirteen active isolates were selected for further study. Microbial strains were identified on the basis of growth conditions and other biochemical characters. Five most active microbial strains were identified using 16S rRNA sequence homology and designated as Streptomyces xanthophaeus MTCC 11938, Streptomyces variabilis MTCC 12266, Streptomyces xanthochromogenes MTCC 11937, Streptomyces levis EU 124569, and Streptomyces sp. NCIM 5500. Four antibacterial and three antifungal compounds isolated from the above five isolates were purified and partially characterized using UV absorption and IR spectra. Two antibacterial metabolites, belong to chromone and peptide antibiotic, respectively. The antifungal compounds were found to be of non-polyene nature. In conclusion, we study the isolation of novel bacterial strains of actinomycetes for producing novel compounds having antibacterial and antifungal activities from the unexplored agro-ecological niches of India. Also, this study paves the way for further characterization of these isolates of Streptomyces sp. for their optimum utilization for antimicrobial purposes.

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Section: Discussionmentioning

confidence: 99%

Isolation, Screening, and Identification of Novel Isolates of Actinomycetes from India for Antimicrobial Applications

et al. 2016

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“…Because of the clinical importance of this compound, the increase in CA production has been the focus of several studies. Strategies to enhance CA production include optimization of batch or fed-batch operation [ 92 , 93 ], temperature [ 94 , 95 , 96 ], agitation and aeration [ 60 , 93 ], and medium composition [ 70 , 94 , 95 ], as well as the selection of new microbial strains [ 97 ].…”

Section: Production Of β-Lactamase Inhibitorsmentioning

confidence: 99%

“…Soybean derivatives (flour, protein isolate, and meal) have been used as nitrogen sources for CA production [ 7 , 67 , 70 , 94 , 95 , 97 , 106 , 107 ]. To perform a screening of medium constituents for fermentative CA production by S. clavuligerus , Rodrigues et al [ 70 ] performed fermentations using soybean protein isolate (SPI) and soybean flour (SF) as the primary nitrogen source, and obtained higher CA concentration (437 mg/L) with the former ingredient.…”

Section: Production Of β-Lactamase Inhibitorsmentioning

confidence: 99%

Production of β-Lactamase Inhibitors by Streptomyces Species

et al. 2018

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β-Lactamase inhibitors have emerged as an effective alternative to reduce the effects of resistance against β-lactam antibiotics. The Streptomyces genus is known for being an exceptional natural source of antimicrobials and β-lactamase inhibitors such as clavulanic acid, which is largely applied in clinical practice. To protect against the increasing prevalence of multidrug-resistant bacterial strains, new antibiotics and β-lactamase inhibitors need to be discovered and developed. This review will cover an update about the main β-lactamase inhibitors producers belonging to the Streptomyces genus; advanced methods, such as genetic and metabolic engineering, to enhance inhibitor production compared with wild-type strains; and fermentation and purification processes. Moreover, clinical practice and commercial issues are discussed. The commitment of companies and governments to develop innovative strategies and methods to improve the access to new, efficient, and potentially cost-effective microbial products to combat the antimicrobial resistance is also highlighted.

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“…DAUFPE 3060, kindly provided by the Microorganism Collection of the Department of Antibiotics of the Federal University of Pernambuco, Recife-PE, Brazil, was recently submitted to molecular identification that revealed 96% identity of the 1,550 bp sequenced fragment with that of the strain Streptomyces variabilis 7525 (accession number: gi|341793371|JN180216.1); therefore, it was identified as Streptomyces variabilis DAUFPE 3060. 24 The microorganism was stored in cryotubes using 10% (v/v) glycerol as cryoprotectant at 2708C. Before its use in extractive fermentations, optical microscopy observations and cell viability tests were done to ensure that its characteristics had been preserved during cryopreservation.…”

Section: Microorganismmentioning

confidence: 99%

Effect of aeration and agitation on extractive fermentation of clavulanic acid by using aqueous two‐phase system

Marques

Santos-Ebinuma

Pessoa

et al. 2016

Biotechnology Progress

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In this work, the effects of agitation and aeration rates on aqueous two-phase system (ATPS)-based extractive fermentation of clavulanic acid (CA) by Streptomyces variabilis DAUFPE 3060 were investigated through a 2 full factorial design, where oxygen transfer rate (OTR) and oxygen uptake rate (OUR) were selected as the responses. Aeration rates significantly influenced cell growth, OUR, and CA yield, while OTR was practically the same in all the runs. Under the intermediate agitation (950 rpm) and aeration conditions (3.5 vvm) of the central point runs, it was achieved OTR of 1.617 ± 0.049 mmol L h , OUR of 0.132 ± 0.030 mmol L h , maximum CA production of 434 ± 4 mg L , oxygen mass transfer coefficient of 33.40 ± 2.01 s , partition coefficient of 66.5 ± 1.5, CA yield in the top and bottom phases of 75% ± 2% and 19% ± 1%, respectively, mass balance of 95% ± 4% and purification factor of 3.8 ± 0.1. These results not only confirmed the paramount role of O supply, broth composition and operational conditions in CA ATPS-extractive fermentation, but also demonstrated the possibility of effectively using this technology as a cheap tool to simultaneously produce and recover CA. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1444-1452, 2016.

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Screening of wild type Streptomyces isolates able to overproduce clavulanic acid

Cited by 6 publications

References 35 publications

Isolation, Screening, and Identification of Novel Isolates of Actinomycetes from India for Antimicrobial Applications

Isolation, Screening, and Identification of Novel Isolates of Actinomycetes from India for Antimicrobial Applications

Production of β-Lactamase Inhibitors by Streptomyces Species

Effect of aeration and agitation on extractive fermentation of clavulanic acid by using aqueous two‐phase system

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