Improvement of submerged culture conditions to produce colorants by Penicillium purpurogenum

Abstract

Safety issues related to the employment of synthetic colorants in different industrial segments have increased the interest in the production of colorants from natural sources, such as microorganisms. Improved cultivation technologies have allowed the use of microorganisms as an alternative source of natural colorants. The objective of this work was to evaluate the influence of some factors on natural colorants production by a recently isolated from Amazon Forest, Penicillium purpurogenum DPUA 1275 employing statistical tools. To this purpose the following variables: orbital stirring speed, pH, temperature, sucrose and yeast extract concentrations and incubation time were studied through two fractional factorial, one full factorial and a central composite factorial designs. The regression analysis pointed out that sucrose and yeast extract concentrations were the variables that influenced more in colorants production. Under the best conditions (yeast extract concentration around 10 g/L and sucrose concentration of 50 g/L) an increase of 10, 33 and 23% respectively to yellow, orange and red colorants absorbance was achieved. These results show that P. purpurogenum is an alternative colorants producer and the production of these biocompounds can be improved employing statistical tool.

Selection of best conditions of inoculum preparation for optimum performance of the pigment production process by Talaromyces spp. using the Taguchi method

“…These results are higher than those previously reported for the production of pigments by Talaromyces purpurogenus (formerly P. purpurogenum) 28,29 ; however, mycelial disks and spores were used as inoculum on those studies.…”

Fungal Pigments and Their Prospects in Different Industries

“…Optimization of pigment production by simultaneously altering the physical and chemical parameters has been explored by many investigators. Several studies have reported an enhancement of the yield of pigment and biomass from different fungal genera such as Monascus, Penicillium, Fusarium, Alternaria, etc., when the physical and chemical parameters were simultaneously altered [104,133,135,158,[160][161][162][163][164][165][166][167].…”

Imidazolium-based ionic liquids as co-surfactants in aqueous micellar two-phase systems composed of nonionic surfactants and their aptitude for recovery of natural colorants from fermented broth

“…The concentrations of the red colorants in both co-existing phases were estimated by spectrophotometric analysis of the absorbance at 490 nm using a UV-Vis multiplate reader (EnSpire Multimode Plate Reader, Perkin Elmer). The wavelength was defined according to the maximum absorbance [32,33]. The results are expressed in "units of absorbance at 490 nm" (UA 490 nm ).…”