An improved purification procedure for Leishmania RNA virus (LRV)

Manzine, Lívia Regina; Silva, Marcos Vinicius G. da; Bettini, Jefferson; Portugal, R.V.; Cruz, Ângela K.; Arruda, Eurico; Thiemann, Otávio Henrique

doi:10.1590/s1517-83822014000200044

Cited by 4 publications

(2 citation statements)

References 11 publications

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“…The role of endosymbiotic LRV has been documented in the development of different clinical manifestations and disease severity of Leishmania infection [ 23 , 24 ] and it has been reported that it leads to the development of severe and disfiguring MCL [ 21 ]. The LRV is a double-stranded RNA virus belonging to the Totiviridae family [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Leishmania RNA virus in Leishmania parasites in patients with tegumentary leishmaniasis: A systematic review and meta-analysis

et al. 2022

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Background Cutaneous leishmaniasis is caused by different protozoan parasites of the genus Leishmania. Leishmania RNA virus (LRV) was identified as the first Leishmania infecting virus in 1998. Different studies showed the presence and role of the LRV in Leishmania parasites causing cutaneous leishmaniasis (CL). However, there is limited data on the pooled prevalence of LRV in Leishmania parasites causing CL. Therefore, the aim of this systematic review and meta-analysis was to determine the pooled prevalence of LRV in Leishmania parasite isolates and/or lesion biopsies in patients with CL from the available literature globally. Methodology We retrieved the studies from different electronic databases. The studies were screened and identified based on the inclusion and exclusion criteria. We excluded studies exclusively done in experimental animals and in vitro studies. The review was conducted in line with PRISMA guidelines. The meta-analysis was performed with Stata software version 14 with metan command. The forest plot with random-effect model was used to estimate the pooled prevalence with 95% confidence interval. Inverse variance index (I2) was used to assess the heterogeneity among the included articles. Principal findings A total of 1215 samples from 25 studies were included. Of these, 40.1% (487/1215) were positive for LRV. The overall pooled prevalence of LRV globally was 37.22% (95% CI: 27.54% - 46.90%). The pooled prevalence of LRV in the New World (NW) and Old World (OW) regions was 34.18% and 45.77%, respectively. Leishmania guyanensis, L. braziliensis, L. major, and L. tropica were the most studied species for the detection of LRV. The prevalence of LRV from Leishmania isolates and lesion biopsies was 42.9% (349/813) and 34.3% (138/402), respectively. Conclusion This systematic study revealed that there is high prevalence of LRV in Leishmania parasites isolated from patients with CL. More comprehensive studies would be required to investigate the presence of the LRV in other Leishmania species such as L. aethiopica to fully understand the role of LRV in different clinical manifestations and disease pathology presented in CL patients.

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Section: Introductionmentioning

confidence: 99%

Prevalence of Leishmania RNA virus in Leishmania parasites in patients with tegumentary leishmaniasis: A systematic review and meta-analysis

et al. 2022

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“…Also, virus particles were isolated form these cells using CsCl gradient ultracentrifugation, and association of dsRNA with these particles was confirmed (Miller et al 1988, Widmer et al 1989 , Khoshnan & Alderete 1993) . A recently developed real time quantitative polymerase chain reaction (RT-qPCR)/ultracentrifugation-based protocol for viral purification couples molecular identification with TEM and has a potential to greatly facilitate viral detection (de Souza et al 2014) .…”

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RNA viruses in trypanosomatid parasites: a historical overview

Grybchuk

Kostygov

Macedo

et al. 2018

Mem. Inst. Oswaldo Cruz

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Viruses of trypanosomatids are now being extensively studied because of their diversity and the roles they play in flagellates’ biology. Among the most prominent examples are leishmaniaviruses implicated in pathogenesis of Leishmania parasites. Here, we present a historical overview of this field, starting with early reports of virus-like particles on electron microphotographs, and culminating in detailed molecular descriptions of viruses obtained using modern next generation sequencing-based techniques. Because of their diversity, different life cycle strategies and host specificity, we believe that trypanosomatids are a fertile ground for further explorations to better understand viral evolution, routes of transitions, and molecular mechanisms of adaptation to different hosts.

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