Secretion of laccase and manganese peroxidase by Pleurotus strains cultivate in solid-state using Pinus spp. sawdust

Abstract

Pleurotus species secrete phenol oxidase enzymes: laccase (Lcc) and manganese peroxidase (MnP). New genotypes of these species show potential to be used in processes aiming at the degradation of phenolic compounds, polycyclic aromatic hydrocarbons and dyes. Hence, a screening of some strains of Pleurotus towards Lcc and MnP production was performed in this work. Ten strains were grown through solid-state fermentation on a medium based on Pinus spp. sawdust, wheat bran and calcium carbonate. High Lcc and MnP activities were found with these strains. Highest Lcc activity, 741 ± 245 U gdm−1 of solid state-cultivation medium, was detected on strain IB11 after 32 days, while the highest MnP activity occurred with strains IB05, IB09, and IB11 (5,333 ± 357; 4,701 ± 652; 5,999 ± 1,078 U gdm−1, respectively). The results obtained here highlight the importance of further experiments with lignocellulolytic enzymes present in different strains of Pleurotus species. Such results also indicate the possibility of selecting more valuable strains for future biotechnological applications, in soil bioremediation and biological biomass pre-treatment in biofuels production, for instance, as well as obtaining value-added products from mushrooms, like phenol oxidase enzymes.

High‐performance of Agaricus blazei fungus for the biological pretreatment of elephant grass

“…(2%), ground wheat bran (2%), CaCO 3 (0.2%), agar (2%) and distilled water (100 mL). Cultures were incubated for 7 days at 28°C ± 2°C until complete colonisation of the media by fungi . Subsequently, plates were used for the preparation of inoculum pre‐treatment.…”

“…The media were homogenised and kept under agitation for 30 min at 24°C ± 2°C. The content was centrifuged at 3,220 g for 15 min for the separation of solid and liquid components and the liquid fraction was collected for later use in enzymatic assays; the solid fraction was discarded …”

“…In the pre‐treatment with the combination of two macromycetes, two discs were inoculated, one with each fungus. The bags were kept at 28°C ± 2°C, for a period of 45 days, and bags were made of 3 collections of each pre‐treatment every 5 days.…”

Fungal pretreatment of sugarcane bagasse: a green pathway to improve saccharification and ethanol production

“…The differences observed during growth may be due to several factors, among others rstly to the characteristics of the strain, the presence of macronutrients and micronutrients required for the species, the type of substrate and the pH of the medium [26], [44]. Biological pretreatment has been associated with modi cations to the biomass via the action of enzymes produced during colonization.…”

Determination of Pleurotus abieticola ligninolytic activity on Norway spruce wood

“…: Fr.) Kummer on wood of deciduous trees and of Pleurotus abieticola Petersen and Hughes on wood of Norway spruce (Schwarze et al 2000;Kodrik 2001;Albertó et al 2002;Żółciak 2002;Szczepkowski and Piętka 2008), (ii) potent ligninolytic enzyme system that enables successful degradation of lignin and various aromatic compounds in wood (Cohen et al 2002;Stajić et al 2004;Camassola et al 2013).…”