Enhanced production of Aspergillus tamarii lipase for recovery of fat from tannery fleshings

Aspergillus niger MH078571.1 and A. niger MH079049.1 were identified previously as the two highest Aspergillus niger strains producing lipase. Biochemical characterizations of lipase activity and stability for these two strains were examined and revealed that the optimal temperature is 45 °C at pH 8for A. niger MH078571.1 and 55 °C for MH079049.1. The lipase production of both strains was studied on medium contains waste oil, as a cheap source to reduce the industrial cost, showed that the optimal incubation period for the enzyme production is 3 days. Moreover, an experiment on lipase activates in organic solvents demonstrated that 50% of acetone is the best solvent for the two strains. In the presence of surfactants, 0.1% of tween 80 surfactant showed the best lipase activities. Furthermore, Mg2+ and Zn2+ ions enhanced the lipase activity of A. niger MH078571.1, while Na2+ and Cu2+ enhanced the enzyme activity of A. niger MH079049.1. Lipase activity was also tested for industrial applications such as integrating it with different detergents. Maximum lipase activity was obtained with 1% of Omo as a powder detergent for both strains. In liquid detergent, 0.1% of Fairy showed maximum lipase activity in A. niger MH078571.1, while the lipase in A. niger MH079049.1 was more effective in 1% of Lux. Moreover, the degradation of natural animal fat with crude enzyme was tested using chicken and sheep fats. The results showed that more than 90% of fats degraded after 5 days of the incubation period.

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“…To estimate the amount of free fatty acids released during each treatment, titrimetric assay was used following Dayanandan et al 32 procedure.…”

Section: Methodsmentioning

confidence: 99%

Application and characterization of crude fungal lipases used to degrade fat and oil wastes

Al-Abdalall

Al-Anazi

Aldakheel

et al. 2021

Sci Rep

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“…Lipases from microorganisms are considered better than those obtained from plants or animals because of faster production, higher yields and greater stability. The enzyme production can be enhanced by selection of potent strains, genetic manipulation and optimization of growth conditions (Dayanandan et al 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of a surfactant-compatible lipase from Aspergillus tamarii JGIF06 exhibiting energy-efficient removal of oil stains from polycotton fabric

et al. 2016

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An extracellular lipase with 23,666.66 U/ml/min activity was produced by Aspergillus tamarii JGIF06 under submerged fermentation in mineral salt medium containing coconut oil (2.5 % v/v), tryptone (2 % w/v) and ammonium chloride (2 % w/v), with initial pH of 5 ± 0.2, incubated at 25 °C for 7 days on a rotary shaker at 120 rpm. A 7.9-fold increase in lipase-specific activity was recorded after purification by DEAE Sepharose ion exchange and Sephadex G200 column chromatography. The apparent molecular mass of this enzyme was revealed as 50 kDa by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The optimal lipase activity was recorded at pH 4 and 37 °C. The enzyme revealed broad specificity towards different vegetable oils. The K m and V max of the lipase on olive oil was found to be 330.4 mg and 53,690 U/ml/min, respectively. The lipase activity was stable in the presence of surfactants such as cetrimonium bromide, sodium dodecyl sulphate and Tween 80, and metal ions and reagents such as Ca2+, Ba2+ and 2-mercaptoethanol. However, the activity was greatly reduced in the presence of organic solvents such as chloroform. The stain removal potential of the crude lipase was determined on polycotton fabric pieces stained with peanut oil. Lipase added to cold water alone significantly enhanced the removal of stain by 152 %. The addition of lipase also improved the stain removal efficiency of a commercially available detergent in the presence of either cold (25 ± 2 °C) or hot (65 ± 2 °C) water. The current findings suggest the potentiality of this enzyme for energy-efficient biocatalytic application.

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“…Caracterização da torta e da borra de dendê A análise centesimal da torta de dendê (Tabela 1) mostrou haver uma boa quantidade de proteínas (4,08g 100g -1 ), embora esse valor seja muito inferior àquele encontrado no farelo de trigo (13,8g 100g -1 ) por SILVEIRA & BADIALE-FURLONG (2007), um resíduo bastante recomendado como substrato para o desenvolvimento de fungos, visando à produção de lipases (KUMAR et al, 2011;REHMAN et al, 2011;TOSCANO et al, 2013;DAYANANDAN et al, 2013). O teor de lipídeos na torta de dendê (7,81g 100g -1 ) pode ser considerado satisfatório para promover a indução e a síntese de Ciência Rural, v.46, n.4, abr, 2016.…”

Section: Resultsunclassified

Aproveitamento de resíduos da agroindústria do óleo de dendê para a produção de lipase por Aspergillus Níger

et al. 2015

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Enhanced production of Aspergillus tamarii lipase for recovery of fat from tannery fleshings

Cited by 24 publications

References 27 publications

Application and characterization of crude fungal lipases used to degrade fat and oil wastes

Application and characterization of crude fungal lipases used to degrade fat and oil wastes

Purification and characterization of a surfactant-compatible lipase from Aspergillus tamarii JGIF06 exhibiting energy-efficient removal of oil stains from polycotton fabric

Aproveitamento de resíduos da agroindústria do óleo de dendê para a produção de lipase por Aspergillus Níger

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