Application and characterization of crude fungal lipases used to degrade fat and oil wastes

Abstract

Aspergillus niger MH078571.1 and A. niger MH079049.1 were identified previously as the two highest Aspergillus niger strains producing lipase. Biochemical characterizations of lipase activity and stability for these two strains were examined and revealed that the optimal temperature is 45 °C at pH 8for A. niger MH078571.1 and 55 °C for MH079049.1. The lipase production of both strains was studied on medium contains waste oil, as a cheap source to reduce the industrial cost, showed that the optimal incubation period for the enzyme production is 3 days. Moreover, an experiment on lipase activates in organic solvents demonstrated that 50% of acetone is the best solvent for the two strains. In the presence of surfactants, 0.1% of tween 80 surfactant showed the best lipase activities. Furthermore, Mg2+ and Zn2+ ions enhanced the lipase activity of A. niger MH078571.1, while Na2+ and Cu2+ enhanced the enzyme activity of A. niger MH079049.1. Lipase activity was also tested for industrial applications such as integrating it with different detergents. Maximum lipase activity was obtained with 1% of Omo as a powder detergent for both strains. In liquid detergent, 0.1% of Fairy showed maximum lipase activity in A. niger MH078571.1, while the lipase in A. niger MH079049.1 was more effective in 1% of Lux. Moreover, the degradation of natural animal fat with crude enzyme was tested using chicken and sheep fats. The results showed that more than 90% of fats degraded after 5 days of the incubation period.

Heterologous expression and characterization of a novel thermostable and alkali stable recombinant lipase enzyme from Bacillus thuringensis into E. coliBL21(DE3) for detergent formulation

“…Recombinant lipase showed stability in the presence of reducing agents as well as inhibitors. These results are in accordance to previous studies (Alabdalall et al, 2021; Prazeres et al, 2006; Rathi et al, 2001; Shoja & Minai‐Tehrani, 2021; Wang et al, 2018). Stability of enzyme in organic solvents is a preferred characteristic for industrial utilization.…”

Promising Abilities of Fungal Lipases of Aspergilli Strains in the Production of Biodiesel from Plant Oil Wastes

“…The supernatant mixtures were also incubated at pH ranges 3, 4, 5, 6, 7, 8, 9, and 10 [31], with 100% and 50% concentrations of organic solvents 1:1 ratio (v/v) for enzyme and organic solvent (methanol, ethanol, isopropanol, butanol, acetone) [32], various concentrations of surfactant solutions (tween 80, tween 20, sodium dodecyl sulfate (SDS)) [33], and metal ions (CaCl 2 , NaCl, KCl, K 4 , NH 4 Cl, Mg SO 4 , Cu SO 4 , ZnSO 4 , EDTA) [31]. These factors have been studied to identify the optimum conditions for the lipase activity and its stability to be used in various industrial and commercial applications [34].…”

Lipase production from Aniba canelilla endophytic fungi, characterization and application of the enzymatic extract

“…The enzymatic extract was characterized according to the methodology described by Alabdalall et al (2021), with modifications. To determine the optimal pH for the lipolytic reaction, the enzyme activity was measured using the following buffer solutions: 50 mM citrate buffer (pH 5.0); sodium phosphate buffer (pH 6.0, 7.0 and 8.0); and 50 mM carbonate buffer (pH 9.0, 10.0 and 10.7).…”