Evaluation of fecal microorganisms of children with cleft palate before and after palatoplasty

Abstract

This study isolated and quantified intestinal bacteria of children with cleft palate before and after palatoplasty. A prospective study was conducted from May 2007 to September 2008 on 18 children with cleft palate, aged one to four years, of both genders, attending a tertiary cleft center in Brazil for palatoplasty, to analyze the effect of surgical palate repair on the concentration of anaerobes Bacteroides sp, Bifidobacterium sp and microaerophiles Lactobacillus sp in feces of infants with cleft palate before and 24 hours after treatment with cefazolin for palatoplasty. There was significant reduction of Lactobacillus sp (p < 0.002), Bacteroides sp (p < 0.001) and Bifidobacterium sp (p = 0.021) after palatoplasty, revealing that surgery and utilization of cefazolin significantly influenced the fecal microbiota comparing collections before and after surgery. However, due to study limitations, it was not possible to conclude that other isolated factors, such as surgical stress, anesthetics and other medications used in palatoplasty might have a significant influence on the microbiota. Considering the important participation of the intestinal microbiota on both local and systemic metabolic and immunological activities of the host, professionals should be attentive to the possible influence of these changes in patients submitted to cleft repair.

Characterizing the microbiota of cleft lip and palate patients: a comprehensive review

“…Meanwhile, Vieira et al studied the presence of Bacteroides spp., Bifidobacterium spp., and Lactobacillus spp. in the feces of CL/P patients before and after surgical revision but did not evaluate their prevalence (Vieira et al, 2013). No doubt, further research is warranted to gain a more comprehensive understanding of the bacteria found in the blood and feces of CL/P patients compared to subjects without CL/P.…”

Salivary microbiome and asthma risk in children with orofacial defects

“…In addition, children with orofacial defects have a split mechanical barrier between the mouth and upper respiratory tract, involving different nasopharyngeal bacterial flora than those in normal children 21 . The gut microbiota of children with CP before and after palatoplasty also changes significantly after surgery or antibiotic use 22 . Continuous respiratory system exposure to the oral microbiome from early life affects the development of mucosal immunity in the airway, opening a window to the potential for developing allergic diseases.…”

“…21 The gut microbiota of children with CP before and after palatoplasty also changes significantly after surgery or antibiotic use. 22 Continuous respiratory system exposure to the oral microbiome from early life affects the development of mucosal immunity in the airway, opening a window to the potential for developing allergic diseases.…”

Efeito de um dentifrício com digluconato de clorexidina a 0,12% sobre a saúde bucal durante o período pós-operatório de pacientes submetidos à cirurgia de enxerto alveolar secundário com rhBMP-2

“…após a intervenção, e portanto a existência de influência do procedimento cirúrgico com indução anestésica a base de cefazolina sobre a microbiota fecal destas crianças. Tendo em vista que a cefazolina também é utlilizada para as cirurgias de enxerto alveolar secundário com rhBMP-2 e a microbiota do trato gastristentinal é continuamente passível de rompimento do equilíbrio, envolvendo a partir disso implicações diversas, torna-se de extrema importância acompanhar a influência da antibioticoterapia sobre a microbiota de todo trato gastrintestinal, o que inclui a bucal, desta população.Ademais a análise genotípica sempre deve ser considerada, tendo em vista que o controle da colonização microbiana está relacionado à disponibilidade e qualidade dos sítios de adesão na mucosa de todo o trato gastrintestinal(VIEIRA et al, 2013), o que possivelmente possui relação com os resultados encontrados nesse estudo.…”