Utilization of Agro-industrial Wastes for the Simultaneous Production of Amylase and Xylanase by Thermophilic Actinomycetes

Abstract

Agro-industrial wastes such as sugarcane bagasse, wheat bran, rice bran, corn cob and wheat straw are cheapest and abundantly available natural carbon sources. The present study was aimed to production of amylase and xylanase simultaneously using agro-industrial waste as the sole carbon source. Seven thermophilic strains of actinomycete were isolated from the mushroom compost. Among of these, strain designated MSC702 having high potential to utilize agro-industrial wastes for the production of amylase and xylanase. Strain MSC702 was identified as novel species of Streptomyces through morphological characterization and 16S rRNA gene sequence. Enzyme production was determined using 1% (w/v) of various agro-industrial waste in production medium containing (g/100mL): K 2 HPO 4 (0.1), (NH 4 ) 2 SO 4 (0.1), NaCl (0.1), MgSO 4 (0.1) at pH 7.0 after incubation of 48 h at 50°C. The amylase activity (373.89 IU/mL) and xylanase activity (30.15 IU/mL) was maximum in rice bran. The decreasing order of amylase and xylanase activity in different type of agro-industrial wastes were found rice bran (RB) > corn cob (CC) > wheat bran (WB) > wheat straw (WS) > sugarcane bagasse (SB) and rice bran (RB) > wheat bran (WB) > wheat straw (WS) > sugarcane bagasse (SB) > corn cob (CC), respectively. Mixed effect of different agroindustrial wastes was examined in different ratios. Enzyme yield of amylase and xylanase was ~1.3 and ~2.0 fold higher with RB: WB in 1:2 ratio.

Optimization of Culture Conditions Using One-Factor-at-Time Methodology and Partial Purification of Amylase from Aspergillus niger of DTO: H5 under Solid State Fermentation

“…When the incubation period was increased, there was a decrease in amylase production. Singh et al, (2012) also reported that Streptomyces sp. MSC702 produced maximum quantity of amylase after an incubation period of 48 h. According to Gupta et al, (2008), the decrease in activity at the later phase of growth probably was because of catabolite repression by glucose which was released from starch hydrolysis.…”

Identification and characterization of a highly alkaline and thermotolerant novel xylanase from Streptomyces sp.

“…showed potential for enzyme production using different agroindustrial residues. It produced maximum xylanases on a medium containing rice bran as a substrate (Singh et al 2012).…”

“…using molecular methods including 16S rRNA sequencing. Singh et al (2012) also identified a novel Streptomyces strain using morphological and molecular characterization including the 16S rRNA gene sequencing.…”

Isolation, Identification and Optimization of Alkaline Amylase Production from Bacillus cereus Using Agro-Industrial Wastes

“…Maximum amylase activity (373.89 IU/ml) was obtained with rice bran at pH 7.0 after incubation of 48 hours by Streptomyces MSC702 in submerged fermentation (Singh et al, 2012). It was investigated that productive medium supplemented with 2% potato starchy waste as a low price substrate could be more favorable than basal medium containing 1% starch for amylases production in submerged fermentation, as it increased α, β and γ amylase activity by 1.98, 7.69 and 12-fold than that produced in basal medium (AbdElhalem et al, 2015).…”