Identification and characterization of a highly alkaline and thermotolerant novel xylanase from Streptomyces sp.

Thomas, Lyn C.; Sindhu, Raveendran; Pandey, Ashok

doi:10.2478/s11756-013-0248-5

Cited by 14 publications

(5 citation statements)

References 22 publications

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“…MDS [ 82 ], and Streptomyces sp. [ 83 ]. In Streptomyces rochei and Streptomyces chromofuscus , xylanase production has been achieved using treated Papyrus and cotton stalk pulp.…”

Section: Diversity Of Lignocellulolytic Actinomycetesmentioning

confidence: 99%

Actinomycetes: A Source of Lignocellulolytic Enzymes

et al. 2015

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Lignocellulose is the most abundant biomass on earth. Agricultural, forest, and agroindustrial activities generate tons of lignocellulosic wastes annually, which present readily procurable, economically affordable, and renewable feedstock for various lignocelluloses based applications. Lignocelluloses are the focus of present decade researchers globally, in an attempt to develop technologies based on natural biomass for reducing dependence on expensive and exhaustible substrates. Lignocellulolytic enzymes, that is, cellulases, hemicellulases, and lignolytic enzymes, play very important role in the processing of lignocelluloses which is prerequisite for their utilization in various processes. These enzymes are obtained from microorganisms distributed in both prokaryotic and eukaryotic domains including bacteria, fungi, and actinomycetes. Actinomycetes are an attractive microbial group for production of lignocellulose degrading enzymes. Various studies have evaluated the lignocellulose degrading ability of actinomycetes, which can be potentially implemented in the production of different value added products. This paper is an overview of the diversity of cellulolytic, hemicellulolytic, and lignolytic actinomycetes along with brief discussion of their hydrolytic enzyme systems involved in biomass modification.

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“…MDS [ 82 ], and Streptomyces sp. [ 83 ]. In Streptomyces rochei and Streptomyces chromofuscus , xylanase production has been achieved using treated Papyrus and cotton stalk pulp.…”

Section: Diversity Of Lignocellulolytic Actinomycetesmentioning

confidence: 99%

Actinomycetes: A Source of Lignocellulolytic Enzymes

et al. 2015

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“…Duarte, Pellegrino, Portugal, Ponezi, and Franco (2000) reported high activity for four strains of B. pumilus, which demonstrated xylanolytic activity of 328, 131, 90 and 167 U mL -1 , respectively. In another study, Bacillus subtilis BE-91 (Liu et al, 2011) was shown to yield 408 U mL -1 , while Streptomyces species (Thomas, Sindhu, & Pandey, 2013b) yielded approximately 200 U mL -1 of xylanase. Since the isolate TC-DT13 was the best xylanase producer in the present screen, it was chosen for further study.…”

Section: Screening Of Xylanolitic Bacteriamentioning

confidence: 95%

Screening of bacterial extracellular xylanase producers with potential for cellulose pulp biobleaching

Rodrigues

Silva

et al. 2019

Acta Sci. Biol. Sci.

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In this study, two hundred fifty-seven bacterial isolates from a suppressive soil library were screened to study their secretion of alkali-thermostable xylanases for potential use in cellulose pulp biobleaching. Xylanase activity was evaluated in solid and liquid media using xylan as the carbon source. Isolates were initially evaluated for the degradation of xylan in solid media by the congo red test. Selected strains were evaluated in liquid media for enzymatic activity and determination of total protein concentration using a crude protein extract (CPE). An isolate identified as Bacillus species TC-DT13 produced the highest amount of xylanase (1808 U mL-1). The isolate was active and stable at 70°C and pH 9.0, conditions which are necessary for the paper industry. This isolate can grow and produce xylanase in medium containing wheat fiber as a substrate. The CPE of this isolate was used in preliminary testing on cellulose pulp bleaching; enzyme treatment of the pulp resulted in a 5% increase of whiteness.

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“…The results of this study were in accordance with previous studies conducted on endoxylanase production by Streptomyces cuspidosporus (Maheswari and Chandra 2000), Streptomyces sp. isolated in India (Thomas et al 2013) and Streptomyces sp. RCK-2010 , which exhibited high endoxylanase production at 25-40 °C.…”

Section: Selection Of Cultural Conditions For Endoxylanase Productionmentioning

confidence: 99%

Valorization of banana pseudostem: endoxylanase production by Streptomyces sp. SH5027 using statistical approaches and its characterization and application in bread making

Adıgüzel

2022

Turkish Journal of Engineering

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Banana pseudostem Endoxylanase Streptomyces RSM Bread makingThe present study aimed to achieve a cost-effective production of endoxylanase by Streptomyces sp. SH5027 using banana pseudostem with the combination of conventional and statistical optimization and to determine the biochemical properties of the enzyme and its effect on bread making. Enzyme production increased from 7.25 U/mL to 50.21 U/mL as a result of the optimization studies. The enzyme was stable at 50-75 °C and also retained more than 50% of its activity at pH 5.0-9.0 for an hour at optimum temperature. The calculated Km value for the purified enzyme was 1.689 mg/mL.min, while the Vmax value was 23.17 µmol/min.mg. The specific volume of the bread increased 9.6%, 12.8%, and 16.8% when 200 U, 300 U, and 400 U endoxylanase was added to the flour per kg, respectively. This study is the first to be conducted on the statistical optimization of endoxylanase production using banana pseudostem.

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Identification and characterization of a highly alkaline and thermotolerant novel xylanase from Streptomyces sp.

Cited by 14 publications

References 22 publications

Actinomycetes: A Source of Lignocellulolytic Enzymes

Actinomycetes: A Source of Lignocellulolytic Enzymes

Screening of bacterial extracellular xylanase producers with potential for cellulose pulp biobleaching

Valorization of banana pseudostem: endoxylanase production by Streptomyces sp. SH5027 using statistical approaches and its characterization and application in bread making

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