Amylase production by endophytic fungi Cylindrocephalum sp. isolated from medicinal plant Alpinia calcarata (Haw.) Roscoe

Abstract

Amylases are among the most important enzymes used in modern biotechnology particularly in the process involving starch hydrolysis. Fungal amylase has large applications in food and pharmaceutical industries.Considering these facts, endophytic fungi isolated from the plant Alpinia calcarata (Haw.) Roscoe were screened for amylolytic activity on glucose yeast extract peptone agar (GYP) medium. Among thirty isolates of endophytic fungi, isolate number seven identified as Cylindrocephalum sp. (Ac-7) showed highest amylolytic activity and was taken for further study. Influence of various physical and chemical factors such as pH, temperature, carbon and nitrogen sources on amylase production in liquid media were studied. The maximal amylase production was found to be at 30 º C and at pH 7.0 of the growth medium.Among the various carbon and nitrogen sources tested, maltose at 1.5% and Sodium nitrate at 0.3% respectively gave optimum amylase production.

Diversity and extracellular enzyme activities of fungal endophytes isolated from medicinal plants of Western Ghats, Karnataka

“…From our laboratory, thirty isolates from Alpinia calcarata Roscoe were screened, of which the isolate Cylindrocephalum sp. gave maximum amylase activity [26]. Among 112 isolates, thirty one isolates have shown cellulolytic activity (27.67%) and the highest cellulolytic activity was demonstrated in Aspergillus sp from P. longum (Table-6).…”

Production, immobilization and thermodynamic studies of free and immobilized Aspergillus awamori amylase

“…Also, El-Banna et al [43] found that K m 415 values and V max of immobilized α-amylase were higher than free enzyme. ... [3] ... [2] ... [4] Page 36 of 44 A c c e p t e d M a n u s c r i p t …”

Improved endoglucanase production and mycelial biomass of some ericoid fungi

“…The production media were also supplemented with either (0.1%, w/v) Na + , K + , Zn 2+ , Mn 2+ , and Co 2+ supplied in sulphate form while 0.1% FeNa-EDTA (ferric EDTA) was supplied as ethylenediamine-tetraacetic acid iron(III) sodium salt to determine the effect of metal ions. Mycelial biomass obtained from each of the treatments was expressed as dry weight after drying in a hot air oven at 80 °C for 16 h (Sunitha et al 2012). Protein content was measured according to the method described by Bradford (1976) using Bovine Serum Albumin (Sigma) as a protein standard.…”