Improved endoglucanase production and mycelial biomass of some ericoid fungi

Abstract

Fungal species associated with ericaceous plant roots produce a number of enzymes and other bio-active metabolites in order to enhance survival of their host plants in natural environments. This study focussed on endoglucanase production from root associated ericoid mycorrhizal and dark septate endophytic fungal isolates. Out of the five fungal isolates screened, Leohumicola sp. (ChemRU330/PPRI 13195) had the highest relative enzyme activity and was tested along with isolates belonging to Hyloscyphaceae (EdRU083/PPRI 17284) and Leotiomycetes (EdRU002/PPRI 17261) for endoglucanase production under different pH and nutritional conditions that included: carbon sources, nitrogen sources and metal ions, at an optimum temperature of 28 °C. An optimal of pH 5.0 produced enzyme activity of 3.99, 2.18 and 4.31 (U/mg protein) for isolates EdRU083, EdRU002 and Leohumicola sp. respectively. Increased enzyme activities and improved mycelial biomass production were obtained in the presence of supplements such as potassium, sodium, glucose, maltose, cellobiose, tryptone and peptone. While NaFe-EDTA and Co2+ inhibited enzyme activity. The potential role of these fungi as a source of novel enzymes is an ongoing objective of this study.

Dark Septate Endophytic Fungi Increase Green Manure-15N Recovery Efficiency, N Contents, and Micronutrients in Rice Grains

“…The positive effects of inoculating plants with DSE fungi are more evident in plants supplied with organic sources of N and P than with inorganic sources (Newsham, 2011 ; Qin et al, 2017 ; Surono and Narisawa, 2017 ; Vergara et al, 2017 ). Organic compounds stimulate the saprophytic character of these fungi, which secrete a series of enzymes, such as endoglucanases, cellulases, amylases, pectinases, and laccases, and secondary metabolites that break complex compounds of carbon (C), nitrogen (N), and phosphorus (P), such as cellulose, starch, protein, and phytate, and convert these into nutrients for host plant absorption (Jumpponen and Trappe, 1998 ; Jumpponen et al, 1998 ; Caldwell et al, 2000 ; Choi et al, 2005 ; Usuki and Narisawa, 2007 ; Upson et al, 2009 ; Mandyam et al, 2010 ; Doolotkeldieva and Bobusheva, 2011 ; Berthelot et al, 2016 ; Adeoyo et al, 2017 ; Surono and Narisawa, 2017 ). Nevertheless, although the literature indicates that DSE fungi can help the host plant to acquire nutrients from organic sources of N, there are still few studies that have evaluated the growth and development of plants supplied with leguminous green manure as a single source of N.…”

Performance of leaf extract media in culturing mycorrhizal mushroom mycelium

“…The mixture was thoroughly stirred with a glass rod and autoclaved at 121 °C and 103.4214 kPa for 15 min in a Classic Prestige Medical autoclave. The autoclaved preparations were allowed to cool to 55 °C and pH was adjusted to 2, 3, 4, 5, 6 or 7 18,29 by addition of 1M HCl or 1M NaOH and thorough stirring with a glass rod 11,33,34 . Pre-run preparation for pH adjustment using 1M HCl or 1M NaOH was done with a dropping pipette to determine the required amounts for each targeted media pH.…”

Highly efficient, processive and multifunctional recombinant endoglucanase RfGH5_4 from Ruminococcus flavefaciens FD-1 v3 for recycling lignocellulosic plant biomasses

“…It is possible that the K + cations play a role in the catalysis of RfGH5_4 affecting its conformation rather than its stability as observed for an endoglucanase from a plant root ericoid mycorrhizal fungus Leohumicola sp. [37,42].…”