Diversity of leaf endophytic fungi in mangrove plants of Northeast Brazil

Costa, Isabella P. M. Wanderley; Maia, Leonor Costa; Cavalcanti, María Auxiliadora de Queiroz

doi:10.1590/s1517-83822012000300044

Cited by 56 publications

(31 citation statements)

References 23 publications

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“…However, few studies can be found in the literature using an isolation temperature of 18°C (Souza et al, 2004). In general, temperatures between 25 and 30°C are commonly used (Lima et al, 2011;Banhos et al, 2014;Bongiorno et al, 2016) or an ambient temperature of 28°C (Costa et al, 2012;Tayung et al, 2012), showing a large variation in this environmental factor (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Antibacterial activity of endophytic fungi isolated from Croton lechleri (Euphorbiaceae)

Vargas¹,

Albuquerque²,

Ribeiro³

et al. 2018

J. Med. Plants Res.

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Croton lechleri is a native species from the Amazon and used with relative frequency in folk medicine in Brazil and other countries. Diversity and antibacterial activity of endophytic fungi associated with this plant were studied here. Samples of leaves and stems were used and 575 endophytic fungi were isolated (307 from leaves and 268 from stems), comprising 284 morphotypes distributed in 13 genera and unknown. The most frequently isolated genera were Phomopsis (30.78%), Penicillium (21.57%) and Pestalotiopsis (16.70%). Diversity and richness of species were higher in leaf tissues. Fifty-five fungi presented antibacterial activity. The fungi with the highest activity were Phomopsis (6.34%), Penicillium (3.17%), and those unknown (5.28%). Penicillium sp. 9 showed the highest antibacterial activity against Enterococcus faecalis and Phomopsis sp. 8 and Phomopsis sp. 9 against Streptococcus pneumoniae and Staphylococcus aureus. Curvularia sp. 1 and a fungus that could not be identified (Unknown sp. 9), showed the highest antibacterial activity against Klebsiella pneumoniae and Escherichia coli, respectively. Only two fungi (Penicillium sp. 9 and Curvularia sp. 1) inhibited the five tested bacteria. Endophytic fungi of C. lechleri harbor a great diversity of endophytic fungi, which have the potential for producing antibacterial compounds.

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Section: Discussionmentioning

confidence: 99%

Antibacterial activity of endophytic fungi isolated from Croton lechleri (Euphorbiaceae)

Vargas¹,

Albuquerque²,

Ribeiro³

et al. 2018

J. Med. Plants Res.

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“…Researchers have reported that Phoma species can be isolated from many plants, such as Laguncularia racemosa (Costa et al, 2012), Amaranthus cruentus (Pusz et al, 2015), Taraxacum mongolicum (Zhang et al, 2013a) and Mitrajyna javanica (Pharamat et al, 2013). An important taxonomic paper from CBS-KNAW (The Netherlands) highlights the importance of a polyphasic approach to characterise Phoma species and similar genera, and it presented an overview of the phytopathological importance of this genus (Aveskamp, 2010).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial activity of Phoma sp. URM 7221: An endophyte from Schinus terebinthifolius Raddi (Anacardiaceae)

Silva¹,

Silvino²,

Bezerra³

et al. 2017

Afr. J. Microbiol. Res.

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The discovery of new metabolites potentially bioactive against pathogenic microorganisms, mainly multidrug resistant, has aroused interest in endophytic fungi. The plant-associated microorganisms have been an important source for development of new compounds of biotechnological interest. This study aimed to investigate the antibacterial capacity of the endophytic fungus, Phoma sp. URM 7221 isolated from the medicinal plant Schinus terebinthifolius against human-pathogenic bacteria. An endophyte was isolated from S. terebinthifolius leaves. Phoma herbarum URM7221 was characterized morphologically and on the basis of ITS rDNA sequence. Primary antimicrobial activity was evaluated using the agar diffusion method and fermentation in liquid medium. Six different solvents were used to extract the active metabolites from fungal biomass and metabolic liquid. An antimicrobial activity test from the extract was carried out using a disk diffusion method with the endophytic extract containing the best antibacterial activity. Two tests were performed: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Partially purified secondary metabolite extracts were analysed by thin layer chromatography (TLC). Liquid metabolically bioactive compounds extracted with petroleum ether revealed a MIC of 25 and 500 μg.mL −1 against S. aureus and MRSA, respectively. Ether and methanol extracts were assessed by chemical analyses and contained phenolic compounds, triterpenes, steroids, reducing sugars, mono-and sesquiterpenes. The thin layer chromatography assay showed the activity of different antimicrobial compounds produced by Phoma sp. URM7221. This endophyte (URM7221) could be efficiently used for production of bioactive metabolites against pathogenic microorganisms, with significant biotechnological potential.

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“…Suryanarayanan et al (2011) measured the biodiversity of endophytic fungi for 33 families of plants present in the forests of India, among them the Anogeissus latifolia species, Terminalia chebula, T. alata and T. crenulata belonging to Combretaceae, in which they found a higher occurrence of the endophytes in genus Phomopsis. Costa et al (2012) isolated 108 fungal strains from the leaves of Laguncularia racemosa, most of them belonging to Guignardia sp. and Phomopsis diachenii.…”

Section: Discussionmentioning

confidence: 99%

“…Diaporthe and its teleomorph Phomopsis are often found as symbionts of Combretaceae (Costa et al 2012;Sebastianes et al 2013;Suryanarayanan et al 2011). M. phaseolina was originally described as a soybean pathogen.…”

Section: Discussionmentioning

confidence: 99%

Endophytism and bioactivity of endophytic fungi isolated from Combretum lanceolatum Pohl ex Eichler

et al. 2016

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We report the isolation and identification of endophytic fungi from Combretum lanceolatum Pohl ex Eichler. Further, we evaluated the relationships of fungi with the host plant and tested bioactivities of isolates. The fungi were isolated from disinfected root fragments and plated onto potato dextrose agar. Root pieces were also used to quantify fungal structures associated with the roots. Identification of fungi was carried out by characterization of morphological features and sequencing of the ITS region. Endophytism was confirmed by inoculation of endophyte-free seedlings followed by microscopic examination. The extract was obtained by maceration of the mycelium in ethyl acetate for antioxidant and antimicrobial evaluations. A total of 112 strains belonging to nine different species were isolated, the major classes were Dothideomycetes and Sordariomycetes. C. lanceolatum is colonized by dark septate endophytes (DSE), evidenced by the presence of microsclerotia and melanized hyphae. There is also co-colonization with mycorrhizal fungi in the same root fragments. Seedling inoculation experiments revealed that C. perangustum-95C and M. phaseolina-46C showed association with the seedlings of C. lanceolatum and differentiated microsclerotia and dark septate hyphae, indicating that these species are DSE. In the antimicrobial test, the D. phaseolorum-92C extract had the highest zones of inhibition against S. aureus and E. coli, respectively. The results showed that 100 % of the extracts have antioxidant activity ranging from low to moderate. All endophyte species had antioxidant and antimicrobial activities that were directly proportional to the dose-responses. Future research will involve chemical characterization and structural elucidation of bioactive compounds.

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Diversity of leaf endophytic fungi in mangrove plants of Northeast Brazil

Abstract: With the aim of increasing the knowledge about endophytic fungi, a group of microorganisms with high biotechnological potential and a valuable source of useful metabolites, a survey in leaves of mangrove

Cited by 56 publications

References 23 publications

Antibacterial activity of endophytic fungi isolated from Croton lechleri (Euphorbiaceae)

Antibacterial activity of endophytic fungi isolated from Croton lechleri (Euphorbiaceae)

Antimicrobial activity of Phoma sp. URM 7221: An endophyte from Schinus terebinthifolius Raddi (Anacardiaceae)

Endophytism and bioactivity of endophytic fungi isolated from Combretum lanceolatum Pohl ex Eichler

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