Bacteriophage amplification assay for detection of Listeria spp. using virucidal laser treatment

Oliveira, I.C.; Almeida, R.C.C.; Hofer, E.; Almeida, P.F.

doi:10.1590/s1517-83822012000300040

Cited by 18 publications

(10 citation statements)

References 7 publications

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“…There are several assay modifications to increase sensitivity of this method. Virucidal laser and toluidine blue O treatment, not damaging for bacterial cells, was shown to be effective for elimination of exogenous bacteriophage interference . Pomegranate rind extract with ferrous sulfate is also proposed as virucidal agent safe for P. aeruginosa , Salmonella typhimurium , and Staphylococcus aureus bacterial cells .…”

Section: Methods Of Detecting Microorganismsmentioning

confidence: 99%

Detection of Microbiological Content in Water as Indicator of Its Ecological State − Mini‐Review

Besarab

Новик

2016

CLEAN Soil Air Water

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Intense anthropogenic pressure on the native media causing grave ecological problems such as deficiency of drinking water, shift of natural evolution of ecosystems due to species extinction, emphasizes the need of accurate analytical systems for continuous environmental monitoring. Microorganisms as simple sensitive living units conducting nutrient metabolic cycles in the environment are applicable for proper assessment of ambient media state. The paper will review modes of microbial contribution in indication and will list important and standard micro-indicators of water pollution. The article will describe up-to-date direct methods of pollutant detection and indirect indicator-recognition techniques. The peculiarities and requirements of water quality monitoring correlated with high dynamics and mobility of aqueous media will be discussed.

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Section: Methods Of Detecting Microorganismsmentioning

confidence: 99%

Detection of Microbiological Content in Water as Indicator of Its Ecological State − Mini‐Review

Besarab

Новик

2016

CLEAN Soil Air Water

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“…This was conducted with Listeria in broth media and also in milk. A virucidal laser was used to destroy any phages that were not bound to Listeria cells in milk, therefore allowing an accurate calculation of original bacterial numbers after a timed incubation (Oliveira et al ). Virucidal reagents have also been used for this application, but their effects should be neutralised before the end of the phage infection cycle (Ayman et al ).…”

Section: Exploitation Of Phages and Phage Proteins For Pathogen Detecmentioning

confidence: 99%

The use of bacteriophages to control and detect pathogens in the dairy industry

O’Sullivan

Bolton

McAuliffe

et al. 2019

Int J of Dairy Tech

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Dairy products are susceptible to bacterial contamination during both primary production and the processing stages. Pasteurisation and high hydrostatic pressure are used to eliminate bacteria from milk; however, some heat‐tolerant pathogens and bacterial spores may survive these processes. Bacteriophages (phages) are viruses that infect and lyse specific bacteria. These or their encoded proteins may be exploited for pathogen biocontrol in milk products and for mastitis treatment during primary production. The host specificity of phages and their proteins may also be exploited for pathogen detection. Various examples of phage‐mediated pathogen biocontrol and detection in the dairy context are discussed in this review.

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“…This technique has the advantage of detecting only viable cells. Therefore, it has most extensively been used for the detection of Campylobacter, Escherichia coli, Listeria, Mycobacterium tuberculosis, Pseudomonas, and Salmonella (Stewart et al, 1998;Pai et al, 2005;Oliveira et al, 2012).…”

Section: Detection Of Bacterial Pathogensmentioning

confidence: 99%

Bacteriophages and their applications

Thung¹,

Lee²,

Premarathne³

et al. 2018

Food Res.

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Bacteriophages are ubiquitous in our world, mainly in the oceans, soil, the water and food we consume. They can be used efficiently in modern biotechnology, as well as alternatives to antibiotics for many antibiotic resistant bacterial strains. Phages can be used as vehicles for vaccines both DNA and protein, for the detection of pathogenic bacterial strain, as biocontrol agents in agriculture and food industry. This review outlines the properties as well as the influence of different external physical and chemical factors like temperature and acidity on phage persistence. A better understanding of the complex problem of phage sensitivity to external factors may be useful for other researchers working with phages. Furthermore, the applications of bacteriophages were described in this paper as well.

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Bacteriophage amplification assay for detection of Listeria spp. using virucidal laser treatment

Cited by 18 publications

References 7 publications

Detection of Microbiological Content in Water as Indicator of Its Ecological State − Mini‐Review

Detection of Microbiological Content in Water as Indicator of Its Ecological State − Mini‐Review

The use of bacteriophages to control and detect pathogens in the dairy industry

Bacteriophages and their applications

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