Isolation of Microsporum gypseum in soil samples from different geographical regions of Brazil, evaluation of the extracellular proteolytic enzymes activities (keratinase and elastase) and molecular sequencing of selected strains

Giudice, Mauro Cintra; Reis-Menezes, Adriana Araujo; Rittner, Glauce Mary Gomes; Mota, Adolfo José da; Gambale, Walderez

doi:10.1590/s1517-83822012000300007

Cited by 19 publications

(10 citation statements)

References 38 publications

(58 reference statements)

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“…Keratinase: The assay was standardized in medium containing keratin azure [37], and the enzymatic quantification was performed according to [38]. One hundred microliters of a yeast suspension containing 4.3 9 10 6 CFU/mL was inoculated into liquid medium with 4 mg/mL of keratin azure and incubated under agitation at 30°C for 14 days.…”

Section: Protease Activitymentioning

confidence: 99%

Enzymatic Activity and Susceptibility to Antifungal Agents of Brazilian Environmental Isolates of Hortaea werneckii

et al. 2015

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Four strains of Hortaea werneckii were isolated from different substrates in Brazil (a salt marsh macrophyte, a bromeliad and a marine zoanthid) and had their identification confirmed by sequencing of the 26S rDNA D1/D2 domain or ITS region. Most of the strains were able to express amylase, lipase, esterase, pectinase and/or cellulase, enzymes that recognize components of plant cells as substrates, but did not express albuminase, keratinase, phospholipase and DNAse, whose substrates are animal-related. Urease production was positive for all isolates, while caseinase, gelatinase and laccase production were variable among the strains. All the strains grew in media containing up to 30% NaCl. We propose that the primary substrate associated with H. werneckii is plant-related, in special in saline environments, where the fungus may live as a saprophyte and decomposer. Infection of animal-associated substrates would be secondary, with the fungus acting as an opportunistic animal pathogen. All strains were resistant to fluconazole and presented high MIC for amphotericin B, while they were susceptible to all the other antifungal agents tested.

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Section: Protease Activitymentioning

confidence: 99%

Enzymatic Activity and Susceptibility to Antifungal Agents of Brazilian Environmental Isolates of Hortaea werneckii

et al. 2015

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“…Nor are the human pathogens a preferred choice as sources of genes for recombinant expression of industrially relevant enzymes because the resulting enzymes may have strong inherent health risks. The keratinase activities of some of these fungi have been partly characterized without identification of the involved genes (Cheung and Maniotis 1973 ; Giudice et al 2012 ). Some proteases of these fungi have also been purified or recombinantly expressed to investigate their role in infection but not for investigation of their potential use as industrial keratinases (Asahi et al 1985 ; Brouta et al 2002 ; Chen et al 2010 ; Lee et al 1987 ; Sriranganadane et al 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Genome and secretome analyses provide insights into keratin decomposition by novel proteases from the non-pathogenic fungus Onygena corvina

Huang

Busk

Herbst

et al. 2015

Appl Microbiol Biotechnol

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Poultry processing plants and slaughterhouses produce huge quantities of feathers and hair/bristle waste annually. These keratinaceous wastes are highly resistant to degradation. Onygena corvina, a non-pathogenic fungus, grows specifically on feathers, hooves, horn, and hair in nature. Hence, the proteases secreted by O. corvina are interesting in view of their potential relevance for industrial decomposition of keratinaceous wastes. We sequenced and assembled the genome of O. corvina and used a method called peptide pattern recognition to identify 73 different proteases. Comparative genome analysis of proteases in keratin-degrading and non-keratin-degrading fungi indicated that 18 putative secreted proteases from four protease families (M36, M35, M43, and S8) may be responsible for keratin decomposition. Twelve of the 18 predicted protease genes could be amplified from O. corvina grown on keratinaceous materials and were transformed into Pichia pastoris. One of the recombinant proteases belonging to the S8 family showed high keratin-degrading activity. Furthermore, 29 different proteases were identified by mass spectrometry in the culture broth of O. corvina grown on feathers and bristle. The culture broth was fractionated by ion exchange chromatography to isolate active fractions with five novel proteases belonging to three protease families (S8, M28, and M3). Enzyme blends composed of three of these five proteases, one from each family, showed high degree of degradation of keratin in vitro. A blend of novel proteases, such as those we discovered, could possibly find a use for degrading keratinaceous wastes and provide proteins, peptides, and amino acids as valuable ingredients for animal feed.Electronic supplementary materialThe online version of this article (doi:10.1007/s00253-015-6805-9) contains supplementary material, which is available to authorized users.

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“…A similar high incidence (40%) of M. gypseum complex has been recorded in the soils of Nevis 12 . M. gypseum has also been found to occur frequently in soils of Brazil, 20.8% in one survey 2 , and 19.2% in another very recent study of 692 samples from different geographic regions of the country 8 . The distribution of geophilic dermatophytes is influenced by soil pH and climatic factors.…”

Section: Discussionmentioning

confidence: 88%

A Preliminary Study on the Occurrence of Keratinophilic Fungi in Soils of Jamaica

Gugnani

Sharma

Wright

2014

Rev. Inst. Med. trop. S. Paulo

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This report represents the first study of keratinophilic fungi present in soils of Jamaica. Out of the 40 soil samples examined from different habitats, 30 (75%) were positive for the presence of keratinophilic fungi, yielding 36 isolates of keratinophilic fungi. Microsporum gypseum complex (represented by 16 isolates of M. gypseum, and four of M. fulvum) was most frequent, being present in 50% of the samples. A very high occurrence of this dermatophyte in Jamaican soil is of public health significance. The remaining isolates of keratinophilic fungi were represented by Chrysosporium spp (mainly C. indicum and C. tropicum) and Sepedonium sp.

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Isolation of Microsporum gypseum in soil samples from different geographical regions of Brazil, evaluation of the extracellular proteolytic enzymes activities (keratinase and elastase) and molecular sequencing of selected strains

Cited by 19 publications

References 38 publications

Enzymatic Activity and Susceptibility to Antifungal Agents of Brazilian Environmental Isolates of Hortaea werneckii

Enzymatic Activity and Susceptibility to Antifungal Agents of Brazilian Environmental Isolates of Hortaea werneckii

Genome and secretome analyses provide insights into keratin decomposition by novel proteases from the non-pathogenic fungus Onygena corvina

A Preliminary Study on the Occurrence of Keratinophilic Fungi in Soils of Jamaica

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