Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China

Simujide, Huasai; Chen, Aorigele; Wang, Chunjie; Yu, Li; Tongrige, Bai

doi:10.1590/s1517-83822012000200013

Cited by 17 publications

(15 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All isolates were E. coli, as demonstrated by the positive uidA results, and all were negative for bfpA, LT, aggR, and wzxo 104 / fliC H4 (data not shown). Distribution of VFs varied depending on the host species, with pigs and cattle presenting more diversity of genotypes, while 57 (29.1%) of the isolates from broilers carried eae as the only detected VF, in agreement with previous reports on avian isolates (9).In cattle E. coli, the occurrence of eae (3.9%) and Shiga toxins (7.7%) in this study was lower among our strains than in previous works (10,11,12).In pigs prevalence of VF was also low, with one isolate being pInv positive and two isolates being positive for both stx 2 and ST (Table 1). In fact, a significant association for the concurrent presence of stx 2 and ST was found in both cattle and swine isolates (Fisher's exact test, P Ͻ 0.001).…”

supporting

confidence: 88%

See 1 more Smart Citation

Assessment of Virulence Factors Characteristic of Human Escherichia coli Pathotypes and Antimicrobial Resistance in O157:H7 and Non-O157:H7 Isolates from Livestock in Spain

Rosel

Gómez-Barrero

Porrero

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

eThe distribution of virulence factors (VFs) typical of diarrheagenic Escherichia coli and the antimicrobial resistance (AMR) profiles were assessed in 780 isolates from healthy pigs, broilers, and cattle from Spain. VF distribution was broader than expected, although at low prevalence for most genes, with AMR being linked mainly to host species. P athogenic isolates of Escherichia coli are characterized by the presence of virulence factors (VFs) that can be combined, leading to different pathotypes (1). Six distinct intestinal pathotypes have been differentiated, including enteropathogenic (EPEC), enterotoxigenic (ETEC), enterohemorrhagic (EHEC), enteroaggregative (EAEC), enteroinvasive (EIEC), and diffusely adherent (DAEC) E. coli (2). The main reservoirs for EPEC, EAEC, and EIEC are humans (1, 3). In contrast, EHEC transmission from animals to humans may also occur through fecal contamination of food or water. In addition to the conventional E. coli pathotypes, novel hybrid pathotypes may emerge, as occurred with EAEC/ EHEC O104:H4 of the German outbreak, which spread across several European countries, affecting almost 4,000 people and causing more than 50 deaths (4).Limited information on the distribution of E. coli pathotypes in healthy livestock (especially non-Shiga toxin-producing E. coli [STEC]) and the possible association between the carriage of VF and their antimicrobial resistance (AMR) patterns is available. In this study, we evaluated the distribution of VFs and AMR profiles in E. coli isolates recovered from healthy livestock in Spain.All these E. coli isolates were recovered in 2009 (n ϭ 780) through the Spanish Surveillance Network of Antimicrobial Resistance in Bacteria of Veterinary Origin (VAV Network) (5). Isolate distribution was as follows: 50 O157:H7 E. coli strains from cattle and 730 E. coli strains (278 from pigs, 196 from broilers, and 256 from cattle) belonging to other serotypes (referred to here as non-O157:H7 E. coli regardless whether they were EHEC). These isolates were recovered from pooled samples collected at Spanish slaughterhouses selected according to their slaughter capacity and located in different regions within the country. Isolates were obtained by culturing pooled feces samples from pigs (2 animals per pool, 556 individual fecal samples analyzed), cattle (2 animals per pool, 512 individual fecal samples analyzed) and broilers (3 animals per pool, 588 individual fecal samples analyzed). Each pool represented one slaughter batch from one single farm.Samples from pigs, broilers, and cattle were cultured on MacConkey agar plates (6). In addition, cattle feces were processed to obtain E. , bfpA, pInv, and aggR) and the somatic and flagellar antigens of O157:H7 and O104:H4 serotypes were assessed in all isolates using conventional PCR (see Table S1 in the supplemental material). The ␤-D-glucuronidase-encoding gene uidA was also included for E. coli confirmation (7). In addition, the stx 2 PCR product from two swine isolates was sequenced to assess their stx 2 type (8). Al...

show abstract

supporting

confidence: 88%

“…In cattle E. coli, the occurrence of eae (3.9%) and Shiga toxins (7.7%) in this study was lower among our strains than in previous works (10,11,12).…”

contrasting

confidence: 80%

Assessment of Virulence Factors Characteristic of Human Escherichia coli Pathotypes and Antimicrobial Resistance in O157:H7 and Non-O157:H7 Isolates from Livestock in Spain

Rosel

Gómez-Barrero

Porrero

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…In China, several studies focused on virulence gene frequencies in E. coli isolates from food-producing animals [9,[17][18][19] but not from companion animals. The present study explored virulence gene frequencies and the association between virulence gene profiles, phylogenetic group, ST type, and fluoroquinolone resistance among E. coli from dogs and cats in China.…”

Section: Discussionmentioning

confidence: 99%

Association between virulence profile and fluoroquinolone resistance in Escherichia coli isolated from dogs and cats in China

Liu

et al. 2017

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Escherichia coli is not only a commensal organism in humans and animals, but also a causative agent of diarrhea and extraintestinal infections. Information about the relationship between population structure, virulence gene profiles, and fluoroquinolone resistance of E. coli in dogs and cats in China is limited. Methodology: A total of 174 pathogenic and commensal E. coli isolates were evaluated in terms of phylogenetic group, virulence gene profile, sequence types (STs), and fluoroquinolone susceptibility. Results: A total of 46.6% of isolates belonged to phylogenetic group B2. Isolates displayed high resistance to tetracycline (82.2%), amoxicillin/clavulanic acid (73.6%), gentamicin (62.1%), and enrofloxacin (60.9%). fimH (81.6%) was the most prevalent virulence gene, and 83.9% of isolates contained one or more investigated virulence genes. The majority of the investigated virulence genes were more prevalent in fluoroquinolone-susceptible isolates and pathogenic isolates. Multilocus sequence typing (MLST) showed that E. coli isolates analyzed were assigned to 65 STs. Among of them, pathogenic-resistant and pathogenic-susceptible isolates had 44 and 10 STs, respectively, while there were 8 and 3 STs in the commensal resistant and susceptible isolates, respectively. Conclusions: Phylogenetic group B2 was the dominant group, accounting for 46.6% of the isolates. Pathogenic isolates and fluoroquinolonesusceptible isolates possessed more virulence genes. Pathogenic isolates and fluoroquinolone-resistant isolates exhibited high population diversity, and pandemic clone ST131 appeared in 9.8% of isolates.

show abstract

“…Recently, even the healthy dairy cows have been reported to carry virulent genes ( stx2, st and lt ) in E. coli isolated from their dung45. Additionally, the humans are reported to be the prime reservoirs for EAEC, EPEC and EIEC4647, though they remain healthy.…”

Section: Discussionmentioning

confidence: 99%

Microbiome analysis reveals the abundance of bacterial pathogens in Rousettus leschenaultii guano

Banskar

Bhute

Suryavanshi

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Bats are crucial for proper functioning of an ecosystem. They provide various important services to ecosystem and environment. While, bats are well-known carrier of pathogenic viruses, their possible role as a potential carrier of pathogenic bacteria is under-explored. Here, using culture-based approach, employing multiple bacteriological media, over thousand bacteria were cultivated and identified from Rousettus leschenaultii (a frugivorous bat species), the majority of which were from the family Enterobacteriaceae and putative pathogens. Next, pathogenic potential of most frequently cultivated component of microbiome i.e. Escherichia coli was assessed to identify its known pathotypes which revealed the presence of virulent factors in many cultivated E. coli isolates. Applying in-depth bacterial community analysis using high-throughput 16 S rRNA gene sequencing, a high inter-individual variation was observed among the studied guano samples. Interestingly, a higher diversity of bacterial communities was observed in decaying guano representative. The search against human pathogenic bacteria database at 97% identity, a small proportion of sequences were found associated to well-known human pathogens. The present study thus indicates that this bat species may carry potential bacterial pathogens and advice to study the effect of these pathogens on bats itself and the probable mode of transmission to humans and other animals.

show abstract

Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China

Cited by 17 publications

References 30 publications

Assessment of Virulence Factors Characteristic of Human Escherichia coli Pathotypes and Antimicrobial Resistance in O157:H7 and Non-O157:H7 Isolates from Livestock in Spain

Assessment of Virulence Factors Characteristic of Human Escherichia coli Pathotypes and Antimicrobial Resistance in O157:H7 and Non-O157:H7 Isolates from Livestock in Spain

Association between virulence profile and fluoroquinolone resistance in Escherichia coli isolated from dogs and cats in China

Microbiome analysis reveals the abundance of bacterial pathogens in Rousettus leschenaultii guano

Contact Info

Product

Resources

About