16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting

Abstract

Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases. however, to our knowledge, this procedure has not been applied in vitreous samples from suspected endophthalmitis cases. Due to the high incidence of cases not detected by CMCT in vitreous samples, which require urgent medical attention since it could lead to vision loss, in this study we used a procedure based on PCR-DGGE in order to solve this problem.The vitreous samples of 24 patients with postoperative endophthalmitis were obtained mainly by vitrectomy. All vitreous samples were previously analyzed by CMCT and there was no bacterial growth. This study was approved by the ethics review board from our institution, and patients was agreed

PCR Detection and Identification of Bacterial Contaminants in Ocular Samples from Post- Operative Endophthalmitis

“…In preliminary PCR experiments 18 specimens showed a band in agarose gel in a correct fragment size, illustrating the presence of bacterial infection (56.4 %). Fungal infections may have caused endophthalmitis in 14 remaining PCR-negative samples [26]. BLAST analysis of PCR products indicated that the vitreous samples contained 12 bacterial species.…”

Identification of Polybacterial Communities in Patients with Postoperative, Posttraumatic, and Endogenous Endophthalmitis through 16S rRNA Gene Libraries

“…This emphasizes the need for molecular techniques to identify mixed pathogens with higher sensitivity. Very few studies have analyzed the polybacterial community, especially in POE cases (16,31). Hence, through this study, we investigated the polybacterial community of endophthalmitis cases through 16S rRNA gene library construction and sequencing.…”

3D‐printed chip for detection of methicillin‐resistant Staphylococcus aureus labeled with gold nanoparticles

“…16S gene expression was expectedly confirmed in all the tested bacterial strains. In the studies , the 16S rRNA gene for classification and identification of bacteria was used, while the suitability of this approach was discussed. The expression of the 16S rRNA gene demonstrated ribosome formation in cells, thus the presence of live bacterial cultures was found in our samples.…”