Optimization of nutrition factors on chitinase production from a newly isolated Chitiolyticbacter meiyuanensis SYBC-H1

Abstract

The present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil recently, which the name Chitinolyticbacter meiyuanensis SYBC-H1 is proposed. A sequential statistical methodology comprising of Plackett-Burman and response surface methodology (RSM) was applied to enhance the fermentative production of chitinase, in which inulin was firstly used as an effective carbon source. As a result, maximum chitinase activity of 5.17 U/mL was obtained in the optimized medium, which was 15.5-fold higher than that in the basal medium. The triplicate verification experiments were performed under the optimized nutrients levels which indicated that it well agreed with the predicted value

Statistical optimization of medium components for chitinase production byPseudomonas fluorescensstrain HN1205: role of chitinase on egg hatching inhibition of root-knot nematode

“…X 2 (KH 2 PO 4 ), X 3 ((NH 4 ) 2 SO 4 ), X 4 (MgSO 4 ¢7H 2 O) and X 5 (sucrose) are below 90% and therefore considered to be insignificant variables. [37,38] Chitinase production from Streptomyces was inhibited by increased phosphate. [39] The above results showed that the PlackettÀBurman experimental design is a powerful tool for screening factors, which have significant effects on chitinase activity.…”

Applications of Plackett–Burman and Central Composite Design for the Optimization of Novel Brevundimonas diminuta KT277492 Chitinase Production, Investigation of its Antifungal Activity

“…Chitin is usually thought as a kind of renewable polysaccharides and organic nitrogenous substance that only second respectively to cellulose and protein in abundance [ 1 ]. Several studies stated that chitin derivatives are of interest to people due to their diverse important applications.…”

“…The stock culture was maintained on nutrient agar slants at 4 °C and periodically subcultured in the National Research Center, Chemistry of Natural and Microbial Products Dept. 1 .…”

“…The time courses of the chitinase production by B.diminuta KT277492, using the basal production medium were monitored for 168 h after 24 h intervals, fermentation broth was centrifuged and chitinase activity was determined 1 .…”

“…According to 1 Chitin is the second most abundant insoluble biodegradable polymer, which exists naturally in the biosphere as a structural polysaccharide of 1,4-N-acetyl-D-glucosamine. It is highly distributed in nature, as a constituent of insect exoskeleton, shells of crustaceans, fungal cell walls and algae components 2 .…”