Cellulolytic potential of a novel strain of Paenibacillus sp. isolated from the armored catfish Parotocinclus maculicauda gut

Castro, André L M de; Vollú, Renata Estebanez; Peixoto, Raquel S.; Grigorevski-Lima, A. L.; Coelho, R. R. R.; Bon, Elba P. S.; Rosado, Alexandre Soares; Seldin, Lucy

doi:10.1590/s1517-83822011000400048

Cited by 11 publications

(5 citation statements)

References 31 publications

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“…had the optimal CMCase production as shorter as 24 h (Paenibacillus sp. P118 [16]) or similar to our strain P.chitinolyticus CKS1 of about 72 h (Paenibacillus tarimensis L88 [17] and Paenibacillus sp. ME-271 60 h [18]).…”

Section: Cmcase Productionsupporting

confidence: 82%

Carboxymethyl cellulase production from a Paenibacillus sp.

Mihajlovski¹,

Davidovic²,

Carevic³

et al. 2016

Hem Ind

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Cellulases are industrially important enzymes with a potential to convert cellulose into fermentable sugars. Novel bacterial isolate Paenibacillus sp. CKS1 was tested for cellulase activity and the optimal conditions for carboxymethyl cellulase (CMCase) production were determined. Maximum CMCase activity was obtained in the third passage of the bacterial culture after 3 days of incubation at 30 °C. Cellobiose and yeast extract was the optimal source of carbon and nitrogen for induction of CMCase activity. In addition, with initial pH 7 of the medium and 40 ml of working volume in 500 ml culture flasks with shaking at 150 rpm, the maximum CMCase activity in a crude culture supernatant reached value of 0.532±0.006 U/ml. For crude CMCase, optimal temperature was 50 °C and optimal pH 4.8, respectively. HPLC analysis confirmed the bacterium is capable to hydrolise CMC to glucose and other soluble sugars.

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Section: Cmcase Productionsupporting

confidence: 82%

Carboxymethyl cellulase production from a Paenibacillus sp.

Mihajlovski¹,

Davidovic²,

Carevic³

et al. 2016

Hem Ind

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“…Bacterial strains were inoculated in media containing specific substrates to stimulate the activity of each enzyme. To compare the amounts of enzymes produced, the hydrolysis index was evaluated [73][74][75]. The Hydrolysis Index (HI) was expressed as the relationship between the mean diameter of the degradation halo and the mean diameter of colony growth [73,76].…”

Section: Enzymatic Assaysmentioning

confidence: 99%

Exploring the Diversity and Biotechnological Potential of Cultured and Uncultured Coral-Associated Bacteria

et al. 2021

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Coral-associated microbes are crucial for the biology of their hosts, contributing to nutrient cycling, adaptation, mitigation of toxic compounds, and biological control of pathogens. Natural products from coral-associated micro-organisms (CAM) may possess unique traits. Despite this, the use of CAM for biotechnological purposes has not yet been adequately explored. Here, we investigated the production of commercially important enzymes by 37 strains of bacteria isolated from the coral species Mussismilia braziliensis, Millepora alcicornis, and Porites astreoides. In-vitro enzymatic assays showed that up to 56% of the isolates produced at least one of the seven enzymes screened (lipase, caseinase, keratinase, cellulase, chitinase, amylase, and gelatinase); one strain, identified as Bacillus amyloliquefaciens produced all these enzymes. Additionally, coral species-specific cultured and uncultured microbial communities were identified. The phylum Firmicutes predominated among the isolates, including the genera Exiguobacterium, Bacillus, and Halomonas, among others. Next-generation sequencing and bacteria culturing produced similar but also complementary data, with certain genera detected only by one or the other method. Our results demonstrate the importance of exploring different coral species as sources of specific micro-organisms of biotechnological and industrial interest, at the same time reinforcing the economic and ecological importance of coral reefs as reservoirs of such diversity.

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“…Berdasarkan hal ini, maka peluang untuk mendapatkan bakteri selulolitik dari saluran pencernaan ikan gurame cukup besar. Hal ini didukung oleh beberapa penelitian yang menunjukkan bahwa bakteri selulolitik banyak terdapat dalam saluran pencernaan ikan, seperti ikan gurame (Murwantoko et al, 2009;Kurniasih et al, 2012), ikan perisai (De Castro et al, 2011) dan ikan mas, mola, nila (Ganguly & Prasad, 2012).…”

Section: Seleksi Dan Identifikasi Bakteri Selulolitik Pendegradasi Daunclassified

Seleksi dan Identifikasi Bakteri Selulolitik yang Dapat Mendegradasi Serat Kasar Daun Singkong

Mulyasari

Widanarni

Suprayudi

et al. 2015

JPBKP

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ABSTRAKPemanfaatan daun singkong sebagai bahan baku pakan ikan belum dilaksanakan secara optimal karena mengandung selulosa yang cukup tinggi. Tuj uan penelitian ini adalah mendapatkan bakteri yang dapat mendegradasi serat daun singkong dan melakukan identifikasi jenis bakteri selulolitik tersebut. Seleksi bakteri selulolitik dilakukan pada 4 jenis isolat bakteri asal saluran pencernaan ikan gurame yaitu UG3, UG6, UG7, dan UG8 dengan mengukur aktivitas enzim selulase secara kuantitatif pada substrat Carboxy Methyl Cellulose (CMC) dan daun singkong menggunakan metode 3,5-dinitrosalicylic acid (DNS). Identifikasi bakteri dilakukan pada 3 isolat yang memiliki aktivitas enzim selulase tertinggi pada substrat daun singkong berdasarkan morfologi (perwarnaan Gram, motilitas dan bentuk bakteri), uji biokimia (oksidase dan katalase) dan analisis gen (16S-rRNA). Hasil penelitian menunjukkan bahwa dari 4 isolat yang diuji cobakan, isolat UG7 memiliki aktivitas enzim selulase (CMCase) tertinggi (0,0043 U/ ml) sedangkan yang terendah adalah UG3 (0,0018 U/ml). Pada uji coba di substrat daun singkong, aktivitas enzim tertinggi terlihat pada isolat UG3 (0,107 U/ml) dan terendah adalah UG6 (0,077 U/ ml). Hasil identifikasi dari 3 jenis isolat bakteri uji yang memiliki kemampuan mendegradasi daun singkong tertinggi (UG3, UG7, dan UG8) menunjukkan bahwa ketiga jenis isolat tersebut merupakan bakteri jenis Gram positif berbentuk batang (basil). Berdasarkan data base dari GenBank, isolat UG3 memiliki kemiripan sebesar 93% dengan Bacillus clausii, UG7 memiliki kemiripan sebesar 96% dengan Bacillus amyloliquefaciens dan UG8 memiliki kemiripan sebesar 88% dengan Bacillus subtilis.

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Cellulolytic potential of a novel strain of Paenibacillus sp. isolated from the armored catfish Parotocinclus maculicauda gut

Cited by 11 publications

References 31 publications

Carboxymethyl cellulase production from a Paenibacillus sp.

Carboxymethyl cellulase production from a Paenibacillus sp.

Exploring the Diversity and Biotechnological Potential of Cultured and Uncultured Coral-Associated Bacteria

Seleksi dan Identifikasi Bakteri Selulolitik yang Dapat Mendegradasi Serat Kasar Daun Singkong

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