Evaluation of INNO-LiPA mycobacteria v2 assay for identification of rapidly growing mycobacteria

García-Agudo, Lidia; Jesús, Iría; Rodríguez-Iglesias, Manuel; García-Martos, Pedro

doi:10.1590/s1517-83822011000300048

Cited by 12 publications

(5 citation statements)

References 19 publications

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“…Many well-characterized mycobacterial species, including known pathogens associated with human NTM diseases (including M. abscessus M. chelonae M. fortuitum M. mucogenicum (48, 49), were not detected in any of the 143 soils investigated here. In fact, the majority of soil-derived mycobacteria were so distantly related to described mycobacteria that they could not be assigned a species or strain identifier.…”

Section: Resultsmentioning

confidence: 73%

A global survey of mycobacterial diversity in soil

Walsh

Gebert

Delgado‐Baquerizo

et al. 2019

Preprint

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Section: Resultsmentioning

confidence: 73%

A global survey of mycobacterial diversity in soil

Walsh

Gebert

Delgado‐Baquerizo

et al. 2019

Preprint

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“…Several commercial kits, which are based on PCR amplification of selected fragment of 16S or 23S rRNA gene or 16S-23S rRNA spacer region, followed by reverse hybridization on nitrocellulose membrane strips such as GenoType Mycobacterium CM/AS (Hain Lifescience, Nehren, Germany) [117][118][119][120] and INNO-LiPA Mycobacteria (LiPA; Innogenetics, Zwijnaarde, Belgium) [121,122] are available for identification of common pathogenic NTM species with high sensitivity and specificity. Genus Mycobacterium, MTBC and 16 NTM species are identified by INNO-LiPA mycobacteria assay, and it is based on the nucleotide variations in the 16S-23S rRNA spacer region (Figure 4).…”

Section: Molecular-based Identification Methodsmentioning

confidence: 99%

Overview of Non Tuberculosis Mycobacterial Lung Diseases

Diseases¹

2018

Mycobacterium - Research and Development

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Nontuberculosis mycobacteria (NTM) are ubiquitous in nature and opportunistically infect different animals, including humans. Currently, NTM is emerging as an important cause of pulmonary infection among both immunocompromised and immunocompetent persons worldwide. The clinical relevance of pulmonary NTM varies among species while showing geographical heterogeneity in distribution as well as pathogenicity. The outcome of the respiratory NTM disease is a consequence of a complex interplay between microbial factors and host susceptibility. Furthermore, HIV infection, cystic fibrosis, cancer, underlying chronic lung disease and history of tuberculosis (TB) may be associated as risk factors for active nontuberculosis pulmonary diseases (NTMPD). The diagnosis of NTMPD requires the presence of symptoms, radiographic evidences, microscopic observations and definitive laboratory diagnostics. Lung infections resulted from a clinically significant NTM species should be treated with appropriate antimicrobial regimen. M. leprae and M. lepraemurium are uncultivable and require the intracellular milieu for survival and propagation [4]. The obligatory causative agents of the genus Mycobacterium, responsible for TB are classified into Mycobacterium tuberculosis complex (MTC). It comprises M. tuberculosis, M.bovis, M. africanum, M. microti [5], M. canettii [6], M. caprae [7], M. pinnipedii [8], M. mungi [9], M. orygis [10] and M. suricattae [11] species. M. tuberculosis, M. africanum, M. canettii and M. orygis cause TB primarily in human [4, 10], whereas M. bovis [12], M. microti [13], M. caprae [7] M. pinnipedii [8], M. mungi [9] and M. suricattae [11] infect cattle, domestic animals, goats, seals, mongooses and meerkats, respectively, and animal tuberculosis can also be zoonotic [14, 15]. However, geographical variation of the MTC species distribution has been identified. As an example, M. africanum is a common cause of human pulmonary TB (39%) as much as M. tuberculosis (55%) in West Africa [16]. In Ghana, 3% of pulmonary TB cases are represented by M. bovis, while 20% are M. africanum and 73% are M. tuberculosis [17].Other medically important mycobacteria such as M. avium, M. intracellulare complex, M. kansasii, M. marinum, M. fortuitum, M. chelonae complex, M. abscessus and M. scrofulaceum are known as nontuberculosis mycobacteria (NTM) species or atypical mycobacteria or mycobacteria other than tuberculosis (MOTT). They are responsible for diseases including lymphadenitis in children, chronic pulmonary diseases, skin and soft-tissue diseases and infections of the skeletal system [18]. NTM are ubiquitous in nature and are widely distributed in water, soil and animals. Among prevailing NTM species, only a few species have a clinical impact on humans as opportunistic pathogens [19]. M. avium complex (MAC), M. abscessus, M. kansasii, M. fortuitum, M. chelonae, M. szulgai, M. triviale and M. scrofulaceum are common NTM species that cause pulmonary diseases in human [20]. Additionally, M. riyadhense was recently proposed as a cau...

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“…The MGIT-positive specimen was subsequently identified as M. haemophilum by INNO-LiPA MYCOBACTERIA v2, using amplification of the 16S-23S ribosomal rRNA spacer region with the primer sequences TTGTACACA CCGCCCGTCA and CGATGCCAAGGCATCC ACC. 2 The MGIT-positive specimen was also cultured in the Lowenstein-Jensen medium at the temperature of 30 C with haemin supplementation. Neither the brain tissue nor the MGIT-positive specimen cultured on the Lowenstein-Jensen medium grew any organism.…”

Section: Case Reportmentioning

confidence: 99%