Optimization of media components for enhanced production of streptococcus phocae pi80 and its bacteriocin using response surface methodology

Kanmani, P.; Kumar, Rakshak; Yuvaraj, N.; Paari, K. A.; Pattukumar, V.; Arul, V.

doi:10.1590/s1517-83822011000200038

Cited by 11 publications

(7 citation statements)

References 1 publication

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“…The strain Streptococcus phocae PI80 was isolated previously from the gut of the Indian white shrimp [15,16,25]. TCBS was used for enumerating Vibrio parahemolyticus (MTCC-451).…”

Section: Samples and Microorganismsmentioning

confidence: 99%

Biopreservation of Sardinella longiceps and Penaeus monodon Using Protective Culture Streptococcus phocae PI 80 Isolated from Marine Shrimp Penaeus indicus

Paari¹,

Kanmani²,

Satishkumar³

et al. 2011

Probiotics & Antimicro. Prot.

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The influence of Streptococcus phocae PI80 on the shelf life of Sardinella longiceps and Penaeus monodon was investigated by measurement of microbial and chemical analysis after appraising the safety of the protective probiotic culture in wistar rat's model. The results of this safety assessment indicate that oral administration of protective culture does not demonstrate any toxicological effects. Consumption of this LAB strain had no adverse effects on animal's general health status, hematology, blood biochemistry, histology parameters, or on the incidence of bacterial translocation. The effect of Streptococcus phocae is very evident with the reduction of Listeria monocytogenes, Vibrio parahemolyticus, and coliforms. During storage, a marked decline in total volatile base and peroxide value was observed in protective culture-treated samples than the control. This strain looks promising as a protective culture for the preservation of fish products.

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“…The strain Streptococcus phocae PI80 was isolated previously from the gut of the Indian white shrimp [15,16,25]. TCBS was used for enumerating Vibrio parahemolyticus (MTCC-451).…”

Section: Samples and Microorganismsmentioning

confidence: 99%

Biopreservation of Sardinella longiceps and Penaeus monodon Using Protective Culture Streptococcus phocae PI 80 Isolated from Marine Shrimp Penaeus indicus

Paari¹,

Kanmani²,

Satishkumar³

et al. 2011

Probiotics & Antimicro. Prot.

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“…The upper and lower limit of each parameter was specified according to OFAT optimization, as mentioned earlier. The value of α was selected based on a rotatable hypothesis for constant variance at points that were equidistant from the center point and supply equal precision of response evaluation in any direction of the design [ 34 ]. The CCD matrix was constructed with 30 runs.…”

Section: Resultsmentioning

confidence: 99%

“…To observe the emulsion stability, the emulsification index (E) was calculated as: [(volume of the emulsion layer × total volume −1 ) × 100]. As xanthan gum is one of the most widely used commercial bacterial EPS sources regularly used as an emulsifier [ 34 ], the emulsifying property of EPS was compared to other natural standard bacterial EPS xanthan gum (Sigma), along with synthetic surfactant Tween 20 (Sigma).…”

Section: Methodsmentioning

confidence: 99%

Optimization and Characterization of a Novel Exopolysaccharide from Bacillus haynesii CamB6 for Food Applications

et al. 2022

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Extremophilic microorganisms often produce novel bioactive compounds to survive under harsh environmental conditions. Exopolysaccharides (EPSs), a constitutive part of bacterial biofilm, are functional biopolymers that act as a protecting sheath to the extremophilic bacteria and are of high industrial value. In this study, we elucidate a new EPS produced by thermophilic Bacillus haynesii CamB6 from a slightly acidic (pH 5.82) Campanario hot spring (56.4 °C) located in the Central Andean Mountains of Chile. Physicochemical properties of the EPS were characterized by different techniques: Scanning electron microscopy- energy dispersive X-ray spectroscopy (SEM-EDS), Atomic Force Microscopy (AFM), High-Performance Liquid Chromatography (HPLC), Gel permeation chromatography (GPC), Fourier Transform Infrared Spectroscopy (FTIR), 1D and 2D Nuclear Magnetic Resonance (NMR), and Thermogravimetric analysis (TGA). The EPS demonstrated amorphous surface roughness composed of evenly distributed macromolecular lumps. GPC and HPLC analysis showed that the EPS is a low molecular weight heteropolymer composed of mannose (66%), glucose (20%), and galactose (14%). FTIR analysis demonstrated the polysaccharide nature (–OH groups, Acetyl groups, and pyranosic ring structure) and the presence of different glycosidic linkages among sugar residues, which was further confirmed by NMR spectroscopic analyses. Moreover, D-mannose α-(1→2) and α-(1→4) linkages prevail in the CamB6 EPS structure. TGA revealed the high thermal stability (240 °C) of the polysaccharide. The functional properties of the EPS were evaluated for food industry applications, specifically as an antioxidant and for its emulsification, water-holding (WHC), oil-holding (OHC), and flocculation capacities. The results suggest that the study EPS can be a useful additive for the food-processing industry.

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“…phocae PI80 is a potent probiotic strain, which was isolated from the gut of shrimp Penaeus indicus [14]. During the fermentation period, it produced some metabolites including exopolysaccharide and bacteriocin [17,18]. Moreover, S. phocae PI80 was found to exhibit potent probiotic role against vibriosis in shrimp Penaeus indicus and Aeromonas infection in fishes Cyprinus carpio and Tilapia mossambicus [19,20].…”

Section: Resultsmentioning

confidence: 99%

Cryopreservation and microencapsulation of a probiotic in alginate-chitosan capsules improves survival in simulated gastrointestinal conditions

Kanmani

Kumar

Yuvaraj

et al. 2011

Biotechnol Bioproc E

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The aim of the present study was to focus on the impact of two different methods and the effects of cryoprotectants on the survival of a probiotic bacterium, Streptococcus phocae PI80, during storage. For the protection of freeze dried cells, the optimal storage conditions were determined with a high survival rate. After the freeze drying process, all cryoprotectants exhibited a protective effect on cell viability at all storage temperatures. High relative cell viability was observed when cells were incubated at −20°C, which was optimum for the protection of S. phocae PI80. Trehalose was the most promising cryoprotectant at all temperatures during the storage period of bacterial cells. The combination of trehalose + skim milk showed more than 85% survivability compared to other combinations at −20°C for 60 days. In addition, encapsulation of probiotic cells into alginate-chitosan gel capsules showed better survival of S. phocae cells (5.468 ± 0.15 LogCFU/mL) with high bacteriocin activity at −20°C for six months. The cell-loaded microcapsules remained stable when treated with simulated gastric and intestinal fluids. After 6 h in vivo treatment, the capsules were found to be broken, releasing the probiotic cells directly into the intestinal system of rats. Therefore, microencapsulation was found to be the most efficient technique, which not only protected the cells for a longer time but also released the cells into the in vivo intestinal system.

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Optimization of media components for enhanced production of streptococcus phocae pi80 and its bacteriocin using response surface methodology

Cited by 11 publications

References 1 publication

Biopreservation of Sardinella longiceps and Penaeus monodon Using Protective Culture Streptococcus phocae PI 80 Isolated from Marine Shrimp Penaeus indicus

Biopreservation of Sardinella longiceps and Penaeus monodon Using Protective Culture Streptococcus phocae PI 80 Isolated from Marine Shrimp Penaeus indicus

Optimization and Characterization of a Novel Exopolysaccharide from Bacillus haynesii CamB6 for Food Applications

Cryopreservation and microencapsulation of a probiotic in alginate-chitosan capsules improves survival in simulated gastrointestinal conditions

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