Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology

Marques, Daniela de Araújo Viana; Cunha, Márcia Nieves Carneiro da; Araújo, Janete Magalí de; Lima-Filho, José Luiz de; Converti, Attílio; Pessoa, Adalberto; Porto, Ana Lúcia Figueiredo

doi:10.1590/s1517-83822011000200030

Cited by 6 publications

(4 citation statements)

References 35 publications

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“…The mean CA productivity in runs carried out at intermediate aeration and agitation rates was 9.0 6 0.1 mg L 21 h 21 after 48 h of extractive fermentation, which corresponds to $75% of substrate consumption. This value is close to the ones reported for conventional fermentation by the same strain 30 and S. clavuligerus. 46 The mean purification factor under the same conditions (3.8 6 0.1) was higher than that reported by Silva et al 32 (1.5) in PEG/phosphate ATPS.…”

Section: Effects Of Agitation and Aeration Rates On Atps Extractive Fsupporting

confidence: 89%

“…The experimental design was composed of seven runs, including three repetitions of the central point (Table ) needed to calculate the pure error . Consistently with the results of conventional fermentation in shake flasks, maximum CA production was obtained after 48 h of extractive fermentation in the central point runs; thus, this time was selected as a reference for both responses. A linear regression model was employed to predict each response ( Y ):

Y = b_{0} + \sum b_{i} x_{i} + \sum b_{j} x_{j} + \sum b_{ij} x_{i} x_{j}

where b 0 is the intercept coefficient, b i and b j are the linear coefficients, b ij is the interaction coefficient and x i and x j are the coded values of the independent variables, namely the aeration rate ( x 1 ) and the agitation rate ( x 2 ).…”

Section: Methodssupporting

confidence: 54%

“…The experimental design was composed of seven runs, including three repetitions of the central point (Table 1) needed to calculate the pure error. 28 Consistently with the results of conventional fermentation in shake flasks, 29,30 maximum CA production was obtained after 48 h of extractive fermentation in the central point runs; thus, this time was selected as a reference for both responses. A linear regression model was employed to predict each response (Y):…”

Section: Experimental Designmentioning

confidence: 78%

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Effect of aeration and agitation on extractive fermentation of clavulanic acid by using aqueous two‐phase system

Marques

Santos-Ebinuma

Pessoa

et al. 2016

Biotechnology Progress

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In this work, the effects of agitation and aeration rates on aqueous two-phase system (ATPS)-based extractive fermentation of clavulanic acid (CA) by Streptomyces variabilis DAUFPE 3060 were investigated through a 2 full factorial design, where oxygen transfer rate (OTR) and oxygen uptake rate (OUR) were selected as the responses. Aeration rates significantly influenced cell growth, OUR, and CA yield, while OTR was practically the same in all the runs. Under the intermediate agitation (950 rpm) and aeration conditions (3.5 vvm) of the central point runs, it was achieved OTR of 1.617 ± 0.049 mmol L h , OUR of 0.132 ± 0.030 mmol L h , maximum CA production of 434 ± 4 mg L , oxygen mass transfer coefficient of 33.40 ± 2.01 s , partition coefficient of 66.5 ± 1.5, CA yield in the top and bottom phases of 75% ± 2% and 19% ± 1%, respectively, mass balance of 95% ± 4% and purification factor of 3.8 ± 0.1. These results not only confirmed the paramount role of O supply, broth composition and operational conditions in CA ATPS-extractive fermentation, but also demonstrated the possibility of effectively using this technology as a cheap tool to simultaneously produce and recover CA. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1444-1452, 2016.

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Section: Effects Of Agitation and Aeration Rates On Atps Extractive Fsupporting

confidence: 89%

Y = b_{0} + \sum b_{i} x_{i} + \sum b_{j} x_{j} + \sum b_{ij} x_{i} x_{j}

Section: Methodssupporting

confidence: 54%

Section: Experimental Designmentioning

confidence: 78%

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Effect of aeration and agitation on extractive fermentation of clavulanic acid by using aqueous two‐phase system

Marques

Santos-Ebinuma

Pessoa

et al. 2016

Biotechnology Progress

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“…Viana- Marques et al (2011) estudaram a produção de AC por espécies de Streptomyces e obtiveram maiores valores de produção em comparação com os obtidos no presente estudo. Entretanto, esses resultados podem ser justificados devido à fonte de carbono utilizada por esses autores (glicerol), visto que, o glicerol é a fonte de carbono que participa diretamente na biossíntese do ácido clavulânico.…”

Section: Discussionunclassified

Atividade antimicrobiana de moléculas bioativas produzidas por Streptomyces parvulus DPUA 1573 frente a Staphylococcus spp. multirresistentes de mastite bubalina

et al. 2016

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Resumo: A mastite é uma inflamação na glândula mamária que pode acarretar perdas na produção e na qualidade do leite, gerando prejuízos econômicos para a pecuária leiteira. O tratamento é baseado na utilização de antibióticos, sendo, em muitos casos, ineficazes devido à resistência bacteriana já conhecida para esta doença. O objetivo deste trabalho foi selecionar linhagens de Streptomyces spp. produtoras de biocompostos com atividade antimicrobiana frente a isolados do gênero Staphylococcus multirresistentes de búfalas com mastite. Bem como, determinar os melhores parâmetros de produção, e avaliar a produção simultânea de ácido clavulânico. A seleção de Streptomyces spp. com capacidade de produzir compostos com atividade antimicrobiana foi realizada através da técnica bloco de gelose. Dentre as 30 espécies de Streptomyces spp. testadas, o micro-organismo Streptomyces parvulus DPUA 1573 apresentou melhores resultados, sendo capaz de inibir o crescimento de 7 isolados Staphylococcus spp. multirresistentes. Posteriormente, a espécie selecionada Streptomyces parvulus DPUA 1573 foi cultivada em diferentes condições pré-determinadas pelo planejamento fatorial 24, onde as variáveis independentes foram: concentração de soja (0,5; 1,0; 1,5%), glicose (0; 0,5; 1g/L), agitação (150; 200; 250rpm) e temperatura (28; 32; 37°C) e todos os ensaios do planejamento foram monitorados até 120 horas de cultivo. Todas as variáveis independentes influenciaram positivamente no crescimento celular, enquanto que para atividade antimicrobiana apenas as variáveis temperatura e agitação apresentaram efeitos significativos positivos. O líquido metabólito produzido por Streptomyces parvulus DPUA 1573 foi capaz de inibir o crescimento de sete Staphylococcus spp. multirresistentes. As melhores condições de cultivo para a produção de moléculas bioativas por este micro-organismo foi a 37?C, com 250rpm de agitação por período de 72 horas. Nos ensaios que apresentaram atividade antimicrobiana, foi avaliada a produção de ácido clavulânico ao longo do cultivo. A maior concentração de ácido clavulânico foi de 269,84g/L obtidas nas condições de 1,5% de farinha de soja em ausência de glicose no tempo de 96 horas. A linhagem Streptomyces parvulus DPUA 1573 foi eficiente contra Staphylococcus spp. multirresistentes isolados de mastite em búfalas, ainda apresentando concomitantemente produção de ácido clavulânico com o potencial uso farmacêutico.

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Response surface methodology: A non-conventional statistical tool to maximize the throughput of Streptomyces species biomass and their bioactive metabolites

Latha

Sivaranjani

Dhanasekaran

2017

Critical Reviews in Microbiology

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Among diverse actinobacteria, Streptomyces is a renowned ongoing source for the production of a large number of secondary metabolites, furnishing immeasurable pharmacological and biological activities. Hence, to meet the demand of new lead compounds for human and animal use, research is constantly targeting the bioprospecting of Streptomyces. Optimization of media components and physicochemical parameters is a plausible approach for the exploration of intensified production of novel as well as existing bioactive metabolites from various microbes, which is usually achieved by a range of classical techniques including one factor at a time (OFAT). However, the major drawbacks of conventional optimization methods have directed the use of statistical optimization approaches in fermentation process development. Response surface methodology (RSM) is one of the empirical techniques extensively used for modeling, optimization and analysis of fermentation processes. To date, several researchers have implemented RSM in different bioprocess optimization accountable for the production of assorted natural substances from Streptomyces in which the results are very promising. This review summarizes some of the recent RSM adopted studies for the enhanced production of antibiotics, enzymes and probiotics using Streptomyces with the intention to highlight the significance of Streptomyces as well as RSM to the research community and industries.

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Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology

Cited by 6 publications

References 35 publications

Effect of aeration and agitation on extractive fermentation of clavulanic acid by using aqueous two‐phase system

Effect of aeration and agitation on extractive fermentation of clavulanic acid by using aqueous two‐phase system

Atividade antimicrobiana de moléculas bioativas produzidas por Streptomyces parvulus DPUA 1573 frente a Staphylococcus spp. multirresistentes de mastite bubalina

Response surface methodology: A non-conventional statistical tool to maximize the throughput of Streptomyces species biomass and their bioactive metabolites

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