Prevalence of antimicrobial resistance and integrons in Escherichia Coli from Punjab, Pakistan

Muhammad, Idrees; Mussarat, Uzma; Yasmin, Badshah; Qadir, Mehmood; Bokhari, Habib

doi:10.1590/s1517-83822011000200008

Cited by 31 publications

(27 citation statements)

References 13 publications

(26 reference statements)

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“…In Pakistan, the frequencies of nalidixic acid and ciprofloxacin resistance among the E. coli isolated from UTIs were 84.2% and 36.5%, respectively, which is in agreement with our findings [18]. In addition, a study in China showed the frequency of ciprofloxacin resistance to have increased to 59.4% [19].…”

Section: Discussionsupporting

confidence: 91%

Detection of plasmid-mediated qnr genes among the quinolone-resistant Escherichia coli isolates in Iran

Firoozeh

Zibaei

Soleimani-Asl

2014

J Infect Dev Ctries

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Introduction: Plasmid-mediated quinolone resistance, which complicates treatment, has been increasingly identified in Escherichia coli isolates worldwide. The purpose of this study was to identify the plasmid-mediated qnrA and qnrB genes among the quinolone-resistant Escherichia coli isolated from urinary tract infections in Iran. Methodology: A total of 140 Escherichia coli isolates were collected between March and October 2012 from urinary tract infections in Khorram Abad, Iran. All isolates were tested for quinoloe resistance using the disk diffusion method. Also, all quinolone-resistant isolates were screened for the presence of the qnrA and qnrB genes by polymerase chain reaction. Minimum inhibitory concentrations (MICs) of ciprofloxacin for the qnr-positive isolates were determined. Results: One hundred sixteen (82.8%) of 140 Escherichia coli isolates were nalidixic acid-resistant; among them, 14 (12.1%) and 9 (7.8%) were qnrA and qnrB-positive, respectively. Two quinolone-resistant isolates harbored both qnrA and qnrB. Among 63 ciprofloxacin-resistant isolates, 14 (22.2%) and 9 (14.3%) were found to carry qnrA and qnrB genes, respectively. The ciprofloxacin MIC range was 0.25-512 μg/mL for 23 qnr-positive Escherichia coli isolates, 18 of which had MICs values of 4-512 μg/mL. Conclusion: Our study shows that the frequency of plasmid-mediated quinolone resistance genes among E. coli isolates in Iran is high.

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Section: Discussionsupporting

confidence: 91%

Detection of plasmid-mediated qnr genes among the quinolone-resistant Escherichia coli isolates in Iran

Firoozeh

Zibaei

Soleimani-Asl

2014

J Infect Dev Ctries

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“…As previously noted, class 1 integrons were more prevalent than those of class 2 (Johnson et al , 1998; Muhammad et al , 2011; Patti et al , 2008). Similar to a research by Colgan et al (2011), in our study intI1 gene was more frequently detected among UPEC than commensal E. coli isolates, which may contribute for the occurrence and transmission of MDR among UPEC isolates.…”

Section: Discussionsupporting

confidence: 75%

Phylogenetic grouping and pathotypic comparison of urine and fecal Escherichia coli isolates from children with urinary tract infection

Navidinia¹,

Peerayeh²,

Fallah³

et al. 2014

Braz. J. Microbiol.

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The aim of this study was to investigate the phylogenetic background and to assess hlyD (involved in the secretion of haemolysin A) and intI1 (encoding a class 1 integrase) in Escherichia coli isolates derived from urinary and fecal specimens. A total of 200 E. coli isolates was collected from patients presenting with urinary tract infection (UTI) during September 2009 to September 2010 and screened for hlyD and intI1 genes by polymerase chain reaction (PCR). Phylogenetic analysis showed that E. coli is composed of four main phylogenetic groups (A, B1, B2 and D) and that uropathogenic E. coli (UPEC) isolates mainly belong to groups B2 (54%) and D (34%) whereas group A (44%) and D (26%) are predominant among commensal E. coli isolates. In this study, hlyD was present in 26% of UPEC and 2% of commensal E. coli isolates. However, hemolytic activity was detected for 42% of UPEC and 6% of commensal E. coli isolates (p < 0.05). intI1 gene was more frequently expressed in UPEC (24%) in comparison with commensal E. coli isolates (12%). Resistance to aztreonam, co-trimoxazole and cefpodoxime were frequently found among UPEC isolates whereas commensal E. coli isolates were commonly resistant to co-trimoxazole, nalidixic acid and cefotaxime. Concluding, a considerable difference between UPEC and commensal E. coli isolates was observed regarding their phylogenetic groups, presence of class 1 integron and hlyD gene, hemolysin activity and resistance pattern. The detection of class 1 integrons and hlyD gene was higher among UPEC compared with commensal E. coli isolates. These findings may contribute for a better understanding of the factors involved in the pathogenesis of UPEC.

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“…Among EPEC isolates, class 1 integron was significantly associated (Table 2). Class 3 integron was not found in any isolate as reported by other studies (White et al, 2001; Yu et al, 2003; Machado et al, 2005; Skurnik et al, 2005; Su et al, 2006; Vinue et al, 2008; Muhammad et al, 2011; Rezaee et al, 2011). Contradicting to the observation, a single study in Iran showed prevalence of class 3 integron with 26.09% (Kargar et al, 2014).…”

Section: Discussionsupporting

confidence: 70%

Distribution of Integrons and Phylogenetic Groups among Enteropathogenic Escherichia coli Isolates from Children <5 Years of Age in Delhi, India

et al. 2017

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Integrons by means of horizontal gene transfer carry multidrug resistance genes (MDR) among bacteria, including E. coli. The aim of this study was to determine the antibiotic resistance profiles and the genes associated with them, to gain insights in the distribution of phylogroups, prevalence, and characterization of class 1, 2 and 3 integrons among Enteropathogenic E. coli (EPEC) isolates, from children upto 5 years of age from Delhi and National Capital Region (NCR), India. A total of 120 E. coli isolates, including 80 from diarrheagenic E. coli (cases) and 40 from healthy isolates (controls) were recruited in this study. After isolation of E. coli, screening for EPEC was done by conventional multiplex PCR. Antibiotic suseptibility test was performed using disk diffusion method and further confirmed by minimum inhibitory concentration (MICs) by E-test. The presence and characterization of integrons and antimicrobial resistance genes were performed by PCR and DNA sequencing. Phylogeny determination was carried out by quadruplex PCR. EPEC strains were found in 64 of the 80 diarrheagenic cases, out of which 38 were MDR. In the 40 healthy controls, 23 were found to be EPEC strain, out of which only 2 were MDR. Amongst 80 diarrheagenic cases, class 1 integron were observed in 43 isolates, class 2 integron in 12 isolates and 9 isolates were found with co-existence of both. Similarly, in healthy controls; class 1 integron in 9 and class 2 integron in 7 isolates were observed with co-existence in 3 isolates. None of the isolates included class 3 integron. The dfr was the most commonly identified gene cassette within the integron-positive isolates. Phylogenetic studies showed considerable representation of phylogroup B2 in both diarrheagenic cases and healthy controls. This study reiterates the importance of class 1 integron predominantly for acquisition of antibiotic resistance genes among EPEC isolates. Furthermore, it also ascertains the possible association between multidrug resistance and presence of integrons. Approximately 91% of isolates were easily assigned to their respective phylogroups. Assessment of the relationship between antibiotic resistance and dominant phylogroups detected was also attempted. This study also highlights the increased burden of antimicrobial resistance in healthy controls.

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Prevalence of antimicrobial resistance and integrons in Escherichia Coli from Punjab, Pakistan

Cited by 31 publications

References 13 publications

Detection of plasmid-mediated qnr genes among the quinolone-resistant Escherichia coli isolates in Iran

Detection of plasmid-mediated qnr genes among the quinolone-resistant Escherichia coli isolates in Iran

Phylogenetic grouping and pathotypic comparison of urine and fecal Escherichia coli isolates from children with urinary tract infection

Distribution of Integrons and Phylogenetic Groups among Enteropathogenic Escherichia coli Isolates from Children <5 Years of Age in Delhi, India

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