Campylobacter insulaenigrae: first isolation report from South American sea lion (Otaria flavescens, (Shaw, 1800)

González, Mercedes Hernández; Villanueva, María Paz; Debruyne, Lies; Vandamme, Peter; Fernández, Heriberto

doi:10.1590/s1517-83822011000100033

Cited by 10 publications

(6 citation statements)

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“…; González et al . ), in this study, is worthy of note. However, the high prevalence of Campylobacter in the grey seals ( Halichoerus grypus ) inhabiting the breeding colony of the Isle of May in the Firth of Forth (Scotland, UK) may reflect the intense human activity in this area and possible infection from human or livestock sources.…”

Section: Discussionsupporting

confidence: 52%

Evidence of land‐sea transfer of the zoonotic pathogen Campylobacter to a wildlife marine sentinel species

et al. 2014

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Environmental pollution often accompanies the expansion and urbanisation of human populations where sewage and wastewaters commonly have an impact on the marine environments. Here we explored the potential for faecal bacterial pathogens, of anthropic origin, to spread to marine wildlife in coastal areas. The common zoonotic bacterium Campylobacter was isolated from grey seals (Halichoerus grypus), an important sentinel species for environmental pollution, and compared to isolates from wild birds, agricultural sources and clinical samples to characterize possible transmission routes. Campylobacter jejuni was present in half of all grey seal pups sampled (24/50 dead and 46/90 live pups) in the breeding colony on the Isle of May (Scotland), where it was frequently associated with histological evidence of disease. Returning yearling animals (19/19) were negative for C. jejuni suggesting clearance of infection whilst away from the localised colony infection source. The genomes of 90 isolates from seals were sequenced and characterized using a whole-genome multilocus sequence typing (MLST) approach, and compared to 192 published genomes from multiple sources using population genetic approaches and a probabilistic genetic attribution model to infer the source of infection from MLST data. The strong genotype-host association has enabled the application of source attribution models in epidemiological studies of human campylobacteriosis, and here assignment analyses consistently grouped seal isolates with those from human clinical samples. These findings are consistent with either a common infection source or direct transmission of human campylobacter to grey seals, raising concerns about the spread of human pathogens to wildlife marine sentinel species in coastal areas.

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Section: Discussionsupporting

confidence: 52%

Evidence of land‐sea transfer of the zoonotic pathogen Campylobacter to a wildlife marine sentinel species

et al. 2014

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“…Other members of the C. lari group are also isolated from gulls (UPTC, C. volucris [ Kaneko et al 1999 ; Debruyne et al 2010b ]) and other shorebirds, such as plovers, redshanks, dunlins, sandpipers, skuas, albatrosses, and penguins ( C. lari , UPTC, C. subantarcticus [ Waldenstrom et al 2002 , 2007 ; Leotta et al 2006 ; Debruyne et al 2010a ; Ryu et al 2014 ]). The C. lari group strains are also isolated from marine mammals ( C. insulaenigrae , C. lari [ Foster et al 2004 ; Stoddard et al 2007 ; Garcia-Pena et al 2010 ; Gonzalez et al 2011 ]), shellfish ( C. lari , C. peloridis [ Endtz et al 1997 ; Van Doorn et al 1998 ; Debruyne et al 2009 ]), and seawater/fresh water ( C. lari , UPTC [ Obiri-Danso and Jones 1999 ; Obiri-Danso et al 2001 ; Meinersmann et al 2013 ; Khan et al 2014 ]). Even though members of the C. lari group have been isolated from livestock ( Tresierra-Ayala et al 1994 ; Aarestrup et al 1997 ; Harvey et al 1999 ; Scanlon et al 2013 ), this clade is primarily associated with coastal regions and watersheds.…”

Section: Introductionmentioning

confidence: 99%

Comparative Genomics of the Campylobacter lari Group

Miller

Yee

Chapman

et al. 2014

Genome Biology and Evolution

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The Campylobacter lari group is a phylogenetic clade within the epsilon subdivision of the Proteobacteria and is part of the thermotolerant Campylobacter spp., a division within the genus that includes the human pathogen Campylobacter jejuni. The C. lari group is currently composed of five species (C. lari, Campylobacter insulaenigrae, Campylobacter volucris, Campylobacter subantarcticus, and Campylobacter peloridis), as well as a group of strains termed the urease-positive thermophilic Campylobacter (UPTC) and other C. lari-like strains. Here we present the complete genome sequences of 11 C. lari group strains, including the five C. lari group species, four UPTC strains, and a lari-like strain isolated in this study. The genome of C. lari subsp. lari strain RM2100 was described previously. Analysis of the C. lari group genomes indicates that this group is highly related at the genome level. Furthermore, these genomes are strongly syntenic with minor rearrangements occurring only in 4 of the 12 genomes studied. The C. lari group can be bifurcated, based on the flagella and flagellar modification genes. Genomic analysis of the UPTC strains indicated that these organisms are variable but highly similar, closely related to but distinct from C. lari. Additionally, the C. lari group contains multiple genes encoding hemagglutination domain proteins, which are either contingency genes or linked to conserved contingency genes. Many of the features identified in strain RM2100, such as major deficiencies in amino acid biosynthesis and energy metabolism, are conserved across all 12 genomes, suggesting that these common features may play a role in the association of the C. lari group with coastal environments and watersheds.

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“…There are also records of the species entering river mouths (basin of the Uruguay River, basin of the Santa Lucía River, Arroyo Pando and Arroyo Solís in Uruguay) and Atlantic coastal lagunes. The sporadic presence of SASL individuals in the Valdivia River is a phenomenon observed through decades and scientifically documented since 1976 ( González et al , 2011 ). In the last decade, a colony of about 30 to 40 animals was established in a coastal area of the urban course of the Valdivia River (39°47’Southern latitude, 73°15’ Western latitude), with the river being their feeding ground; whilst they use the sandy beach by the river, the lawn and the pavement of the street in the surrounding areas as resting ground.…”

Section: Introductionmentioning

confidence: 99%

“…This prompted us to look for the presence of zoonotic intestinal bacteria in fresh sea lions feces placed on the pavement. During the course of this investigation, Campylobacter insulaenigrae ( González et al , 2011 ) and several species of the Family Enterobacteriaceae were isolated ( González et al , 2010 ), with Edwardsiella tarda being the most frequently found (73%) ( González et al , 2010 ). This is a zoonotic bacterium that has been isolated from humans, animals, and the environment and it is particularly considered a fish pathogen, being the only species of its genus that has been reported to be pathogenic to humans ( Leung et al , 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Adhesive and invasive capacities of Edwarsiella tarda isolated from South American sea lion

Fernández

Villanueva

González

et al. 2014

Braz. J. Microbiol.

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Edwarsiella tarda is a zoonotic bacterium that can be isolated from humans, animals and the environment. Although E. tarda is primarily considered a fish pathogen, it is the only species of its genus considered to be pathogenic for humans as well. A survey of zoonotic intestinal bacteria in fresh feces from South American sea lions (SASL) Otaria flavescens, reported E. tarda as the most frequently isolated species. In this study, we used HEp-2 cells to establish in vitro the adherence and invasive ability of 17 E. tarda strains isolated from SASL fecal material. All the strains were able to adhere and invade HEp-2 cells with adhesion and invasion percentages ranging from 56 to 100% and 21 to 74%, respectively. Despite the expression of these pathogenic factors, further investigation is needed to determine whether this bacterium could play a role as primary pathogen for this and other species of pinnipeds.

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Campylobacter insulaenigrae: first isolation report from South American sea lion (Otaria flavescens, (Shaw, 1800)

Cited by 10 publications

References 9 publications

Evidence of land‐sea transfer of the zoonotic pathogen Campylobacter to a wildlife marine sentinel species

Evidence of land‐sea transfer of the zoonotic pathogen Campylobacter to a wildlife marine sentinel species

Comparative Genomics of the Campylobacter lari Group

Adhesive and invasive capacities of Edwarsiella tarda isolated from South American sea lion

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