Ciprofloxacin susceptibility reduction of Salmonella strains isolated from outbreaks

Souza, Roberta Barreiros de; Ferrari, Rafaela Gomes; Magnani, Marciane; Kottwitz, Luciana Bill Mikito; Alcocer, Iliana; Tognim, Maria Cristina Bronharo; Oliveira, Tereza Cristina Rocha Moreira de

doi:10.1590/s1517-83822010000200033

Cited by 18 publications

(16 citation statements)

References 20 publications

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“…Ceftiofur is a third-generation cephalosporin, and these high levels of resistance represent a public health concern, as after the emergence of strains resistant to chloramphenicol around the 1990s, quinolones and third-generation cephalosporins started to be the antibiotics of choice for the treatment of salmonellosis in humans (Pokharel et al, 2006;Souza et al, 2010b;Lopes 2014).…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Susceptibility and Biofilm Production by Salmonella sp. Strains Isolated from Frozen Poultry Carcasses

Sereno

Ziech

Druziani

et al. 2017

Rev. Bras. Cienc. Avic.

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The objectives of this study were to evaluate the antimicrobial resistance and the biofilm-producing ability of Salmonella sp. strains isolated from frozen poultry carcasses. Antimicrobial susceptibility was tested by the disk-diffusion method. Biofilm-producing ability was determined in 96-well polystyrene microplates stained with crystal violet at 1%. Out of the 22 strains tested, all were multiresistant, that is, resistant to more than three antimicrobial classes, and 72.7% were able to form biofilms. The highest resistance rates obtained were against sulfonamides, tetracycline, and quinolones. On the other hand, 100% of the strains were sensitive to chloramphenicol. According to the rate of biofilm formation, 3 (13.6%) and 13 (59.1%) strains were classified as moderate and weak biofilm-producers, respectively, and 27.3% did not form biofilms. Biofilms increase the tolerance of microorganisms to stress, reducing their sensitivity to disinfectants and antimicrobials; favor equipment corrosion; and act as substrates for the adhesion of bacteria with lower biofilm-producing capacity. The results of the present study stress the importance of cleaning procedures in food processing plants and highlight the public health risks related to the emergence of multiresistant strains.

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Section: Resultsmentioning

confidence: 99%

Antimicrobial Susceptibility and Biofilm Production by Salmonella sp. Strains Isolated from Frozen Poultry Carcasses

Sereno

Ziech

Druziani

et al. 2017

Rev. Bras. Cienc. Avic.

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“…Thus, the use of antimicrobial agents in poultry farming may be the main cause for the increase and spread of resistant Salmonella strains (Giraud et al, 2006;Vaz et al, 2010;Souza et al, 2010).…”

Section: Characterization Of S Enteritidis Pt4 and Pt9mentioning

confidence: 99%

Molecular characterization and resistance profile of Salmonella Enteritidis PT4 and PT9 strains isolated in Brazil

Kottwitz

Scheffer

Dalla-Costa

et al. 2011

Journal of Medical Microbiology

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A total of 41 Salmonella Enteritidis strains, including phago-types (PTs) PT4 and PT9, were characterized by antimicrobial resistance profiles and PFGE. Of these strains, 34 were isolated from patients and foods, and 7 were of poultry origin. All strains were susceptible to ampicillin, chloramphenicol, cefotaxime, ciprofloxacin and trimethoprim/sulfamethoxazole, and 41.5 % (n517) were resistant to nalidixic acid. PFGE analysis using XbaI and SpeI restriction enzymes resulted in X1S1 as the prevalent pattern, which was present in 48.8 % (n520) of epidemic strains and in one strain isolated from discarded hatching eggs. Distinct patterns were found for the other strains isolated from poultry (X3S1, X8S8, X11S12, X11S13, X16S1 and X13S15). The S. Enteritidis PT9 strains associated with outbreaks of salmonellosis were highly similar (¢0.90), suggesting clonality. The PFGE genotypes were related to the PTs, and it was possible to differentiate strains isolated from patients with salmonellosis from other strains of non-epidemic origin. The PFGE results suggested that the S. Enteritidis strains of poultry origin were a possible source of human salmonellosis during the study period. INTRODUCTIONSalmonella species are associated with food-borne diseases worldwide. Foods of animal origin, such as poultry meat, eggs and their products, are frequently implicated in outbreaks (Clavijo et al., 2006). In the period 1999-2008, Salmonella species were responsible for 1408 (23.2 %) of the 6062 outbreaks investigated in Brazil, and Salmonella species were responsible for 267 (43. According to epidemiological data from the Central Laboratory of Parana State (LACEN), egg products were associated with 45.0 % of the salmonellosis outbreaks occurring from 1999 to 2008, and Salmonella enterica subsp. enterica serovar Enteritidis was the predominant serovar found in patient samples (87.8 %) and foods (80.6 %) .Phenotypic methods, such as phago-typing, antimicrobial resistance profiling and molecular methods, including analysis of the macrorestriction pattern of chromosomal DNA after PFGE, have been used to characterize S. Enteritidis strains. PFGE has been widely utilized in the molecular characterization of Salmonella species due to the stability of patterns, reproducibility and excellent discriminatory power (Ribot et al., 2002;Peters et al., 2003;Woo, 2005;Zou et al., 2010).Salmonella strains isolated from different sources in the same temporal period and from the same geographical location are likely to be epidemiologically related and to have an identical PFGE restriction pattern independent of the restriction enzyme employed (Tenover et al., 1995). PFGE is capable of discriminating strains of Salmonella of the same serovar and phago-type (PT) (Ahmed et al., 2000; Liebana et al., 2001).The aim of the present study was to characterize S. Enteritidis PT4 and PT9 strains of poultry origin or isolated from patients and foods involved in salmonellosis outbreaks by antimicrobial resistance profiles and PFGE patterns. An epidemiological co...

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“…Nesse sentido, destacam-se estudos em que foram encontrados isolados de Salmonella spp. com elevadas taxas de resistência no Brasil (Cortez et al,2006;Cardoso et al, 2006;Ribeiro et al, 2006;Duarte et al, 2009;Souza et al, 2010), assim como na Espanha (Carramiñana et al, 2004), Lituânia (Ruzauskas et al, 2005), Estados Unidos (Alali et al, 2010) e Grécia (Sakaridis et al, 2011).…”

Section: Introductionunclassified

Ocorrência e perfil de resistencia antimicrobiana de sorotipos de Salmonella spp. isolados de aviários do Paraná, Brasil

Pandini

Pinto

Muller³

et al. 2015

Arq. Inst. Biol.

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O presente estudo foi conduzido para verificar o perfil de resistência de diferentes sorotipos de Salmonella spp. isolados em aviários de frango de corte frente a agentes antimicrobianos. Foram processados 342 suabes de arrasto provenientes de granjas avícolas do oeste do Paraná, no período de janeiro de 2010 a janeiro de 2011, sendo isoladas 39 amostras de Salmonella spp. Os sorotipos mais frequentes foram: S. Heidelberg, S. Mbandaka, S. Newport, S. Schwarzengrund, S. Enteritidis, S. Livingstone, S. Orion, S. Give e S. Infantis. A determinação do perfil de resistência para os 19 sorotipos de Salmonella identificados foi realizada em relação a 12 antimicrobianos comerciais. Os resultados indicam que 51% dos sorotipos de Salmonella apresentaram resistência a um ou mais antimicrobianos, com 12 diferentes padrões de resistência. O maior percentual de resistência foi verificado à tetraciclina (30,8%), e o menor à gentamicina e cloranfenicol (2,6%). Os níveis de resistência indicam que os antimicrobianos devem ser utilizados nos aviários de forma mais prudente, buscando, assim, minimizar a disseminação de cepas resistentes.

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Ciprofloxacin susceptibility reduction of Salmonella strains isolated from outbreaks

Cited by 18 publications

References 20 publications

Antimicrobial Susceptibility and Biofilm Production by Salmonella sp. Strains Isolated from Frozen Poultry Carcasses

Antimicrobial Susceptibility and Biofilm Production by Salmonella sp. Strains Isolated from Frozen Poultry Carcasses

Molecular characterization and resistance profile of Salmonella Enteritidis PT4 and PT9 strains isolated in Brazil

Ocorrência e perfil de resistencia antimicrobiana de sorotipos de Salmonella spp. isolados de aviários do Paraná, Brasil

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