Antimycobacterial susceptibility testing methods for natural products research

Bueno, Juan; Kouznetsov, Vladímir V.

doi:10.1590/s1517-83822010000200001

Cited by 48 publications

(30 citation statements)

References 65 publications

(81 reference statements)

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“…Even the pharmaceutical industry prefers the development of antibiotics with ≤ 2 µg/mL. Minimum bactericidal activity is greater than MIC value, because the KHM value obtained in this study was 450 µg/mL (or greater than 100 µg/ mL), then the MIC was not determined (Sanchez and Kouznetsov, 2010;Levison and Levison, 2009). …”

Section: Statistic Analysismentioning

confidence: 70%

Efektivitas in vitro Alfa Mangostin Pada Pertumbuhan Bakteri Uropatogen Escherichia coli Multiresisten

Rakhmawatie¹,

Rohmani²,

Ahyar³

2017

Sains Medika

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Background. The incidence of Multi Drug Resistant (MDR) in extraintestinal E. coli has become a global problem in the world. In Indonesia, the greatest resistance to the uropathogen E. coli is resistant to ampicillin (91.9%), ciprofloxacin (83.7%) and cefixime (67.6%). Therefore it takes effort for the treatment of MDR uropathogen E. coli, one of them are development of new antibiotics from herbal isolates of Garcinia mangostana L., a-mangosteen. This study examined the activity of a-mangosteen in vitro on the growth of MDR uropathogen E. coli.Methods. Treatment of uropathogen E. coli is performed in vitro, using a-mangosteen with a range of levels 14 - 450 �g/mL. The antibacterial activity of a-mangosteen is measured by determine at the growth or death of bacteria at each concentration using indirect methods, which is absorbance reading. Uropathogen E. coli that had been treated with various concentration of a-mangosteen incubated for 18-20 hours, then read an absorbance at wavelenght 625 nm using a spectrophotometer.Result. The Minimal Inhibitory Concentration (MIC) of a-mangosteen in this study was 450 �g/mL. Based on the linear regression (STATA 13.1) relationship between concentration of a-mangosteen and activity of growth inhibition of bacteria, obtained the F test value 0.0001 < 0.05, states that all concentrations of a-mangosteen simultaneously have a significant influence on the growth of uropathogen E. coli.Conclusion. MIC value is relatively large causing a-mangosteen activity against Gram-negative bacteria needs to be studied further. Potentially relevant activity in the clinic will occur if the value of in vitro MIC < 100 �g/mL. Likewise, the pharmaceutical industry prefers the development of antibiotics that have in vitro MIC values = 2 �g/mL.

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Section: Statistic Analysismentioning

confidence: 70%

Efektivitas in vitro Alfa Mangostin Pada Pertumbuhan Bakteri Uropatogen Escherichia coli Multiresisten

Rakhmawatie¹,

Rohmani²,

Ahyar³

2017

Sains Medika

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“…This charge also influences the biological activity of nanoparticles in bacterial cell membranes, which are negatively charged (Qi et al, 2004). For that reason, paper disk diffusion bioassays as well as agar diffusion assays are not described because of the difficulties of diffusion of compounds through agar medium and the disadvantages with these platforms being adapted to a system of high-throughput screening (Burygin et al, 2009;Sánchez and Kouznetsov, 2010;Allahverdiyev et al, 2011;Elkhair, 2014). …”

Section: Antimicrobial Susceptibility Testing Methods Of Nmsmentioning

confidence: 99%

Antimicrobial Models in Nanotechnology

Bueno¹

2015

Nanotechnology in Diagnosis, Treatment and Prophylaxis of Infectious Diseases

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“…To obtain the qualitative information on the antimycobacterial activity of the extract, agar well diffusion assay was performed [20,21]. In all these experiments, rifampicin (5 µg) served as the positive control.…”

Section: Tuberculosis Assaymentioning

confidence: 99%

Allium Sativum Linn. Contains Linear Alkylbenzene Sulfonates That Alter Membrane Fluidity for the Inhibition of Mycobacterium Tuberculosis H37ra

Muniyan

Sabareesh

Naz

et al. 2017

Asian J Pharm Clin Res

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Objectives: The purpose of the study is to characterize antimycobacterial phytoconstituent from ethyl acetate extract of dried bulbs of Allium sativum Linn. (Alliaceae) and elucidating the probable mode of action of the bioactive molecule.Methods: Serial extraction, Mycobacterium tuberculosis assay by agar well diffusion method, minimal inhibitory concentration by microplate alamar blue assay, Fourier transform infrared (FT-IR), nuclear magnetic resonance (NMR) spectroscopy, liquid chromatography (LC)-electrospray ionization (ESI)-mass spectrometry (MS)/MS, cell leakage assay, scanning electron microscopy (SEM), inhibition property of linear alkylbenzene sulfonate (LAS) in the presence of rifampicin on M. tuberculosis were performed.Results: Ethyl acetate extract displayed significant inhibition properties against M. tuberculosis H37Ra (MTCC 300). Subsequently, the bioactivityguided fractionation was employed to purify the phytochemical. Analysis of FT-IR, LC-MS (ESI), 1 H, and 13 C-NMR spectrum revealed that the bioactive phytochemicals are the variants of LAS, with C 12 -alkyl being predominant, and the minimum inhibitory concentration was found to be 5.56 μg/ml. Morphological examination by SEM and cell leakage assay indicated that these molecules change the membrane fluidity. Conclusion:The results thus suggest the possibility of using low concentrations of LAS to effect changes in membrane fluidity, thereby enhancing the efficacy of antibiotic treatment.

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Antimycobacterial susceptibility testing methods for natural products research

Cited by 48 publications

References 65 publications

Efektivitas in vitro Alfa Mangostin Pada Pertumbuhan Bakteri Uropatogen Escherichia coli Multiresisten

Efektivitas in vitro Alfa Mangostin Pada Pertumbuhan Bakteri Uropatogen Escherichia coli Multiresisten

Antimicrobial Models in Nanotechnology

Allium Sativum Linn. Contains Linear Alkylbenzene Sulfonates That Alter Membrane Fluidity for the Inhibition of Mycobacterium Tuberculosis H37ra

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