Immobilized Saccharomyces cerevisiae as a potential aflatoxin decontaminating agent in pistachio nuts

Abstract

In this study, we investigated the binding ability of Saccharomayces cerevisiae to aflatoxin in pistachio nuts. The obtained results indicate that S. cerevisiae has an aflatoxin surface binding ability of 40% and 70% (with initial aflatoxin concentrations of 10 and 20 ppb) in the exponential phase. Acid treatments increase this ability to approximately 60% and 73% for the two concentrations of aflatoxin, respectively.Heat treatments also enhance surface binding to 55% and 75%, respectively. Binding appears to be a physical phenomenon that saturates within the first 2-3 hours of the process. The obtained results indicate that yeast immobilization for toxin reduction on aflatoxin-contaminated pistachios had no effect on qualitative characteristics, such as color, texture, and peroxide value. Yeast cells, viable or nonviable, are effective for aflatoxin binding, and this property could lead to a promising solution to aflatoxin contamination in high-risk foods.

Immobilization of Saccharomyces cerevisiae on Perlite Beads for the Decontamination of Aflatoxin M1 in Milk

“…Among the different decontaminating microorganisms, Saccharomyces cerevisiae has been widely used in AF decontamination due to the special structure of the cell wall (Abyaneh, ). Recently, there has been a number of in vitro studies on AF decontamination in foodstuffs using S. cerevisiae (Corassin, Bovo, Rosim, & Oliveira, ; Gonçalves, Rosim, de Oliveira, & Corassin, ; Karazhiyan, Mehraban Sangatash, Karazhyan, Mehrzad, & Haghighi, ; Prado et al., ; Rahaie, Emam‐Djomeh, Razavi, & Mazaheri, ).…”

“…Immobilization of S. cerevisiae has provided a decontamination process with a higher performance because of its practical benefits compared with a traditional free cell system (Rahaie et al., ). The cell immobilization technique provides a continuous decontamination process as well as higher yeast cell densities and metabolic activity in the biofilter, which leads to a shorter AF decontamination time (Verbelen, De Schutter DP, Verstrepen, & Delvaux, ).…”

Recent Trends in Microbiological Decontamination of Aflatoxins in Foodstuffs

“…On the other hand, acid conditions may affect polysaccharides by releasing monomers, which are further fragmented in aldehydes after glycosidic bonds are broken. Continuous removal of aflatoxin, even after use of acid and heat treatments, confirms that yeast cell viability is not a significant factor for the removal of aflatoxin from the medium [69].…”

“…Immobilized SC cells (ATTC 9763) was investigated for their ability to remove AFB 1 from pistachio seeds, and it was observed that the amount of toxin removed was dependent on its concentration in the medium (40% and 70% of removal for concentrations of 10 ng/mL and 20 ng/mL AFB 1 , respectively) [69]. The authors also concluded that this ability to remove the toxin was greater in SC exponential growth phase, and that the process was a quick one, being saturated after 3 hours of contact.…”

The use ofSaccharomyces cerevisiaeimmobilized on activated alumina, and alumina silicate beads for the reduction of Aflatoxin M1in vitro

“…The immobilization of S . cerevisiae has provided a decontamination process with a higher performance because of its practical benefits compared with a traditional free cell system (Foroughi et al ; Rahaie et al ). The cell immobilization technique provides a continuous decontamination process as well as higher yeast cell densities and metabolic activity in the biofilter, which leads to a shorter AF decontamination time (Verbelen et al ).…”