Recent Trends in Microbiological Decontamination of Aflatoxins in Foodstuffs

Oliveira, Carlos Augusto Fernandes de; Campagnollo, Fernanda B.; Corassin, Carlos Humberto; Jager, Alessandra Vincenzi; Reddy, K. R. N.

doi:10.5772/51120

Cited by 12 publications

(10 citation statements)

References 83 publications

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“…Under European climatic conditions, AFs can be nested, for example in certain layers of silos heated by the sun or in hot hay. The presence of mycotoxins in livestock is still a problem, especially as there is no monitoring of the level of contamination (Coffey et al 2009 ; Dalie et al 2010 ; Oliveira et al 2013 ; Rubert et al 2014 ).…”

Section: Undesirable Microflora and Its Sources In Plant Materials Intmentioning

confidence: 99%

Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a minireview

Fabiszewska

Zielińska

Wróbel

2019

World J Microbiol Biotechnol

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Ensiling is one of the best known method to preserve fodder. The forage before ensiling intended for silages usually contains a low number of lactic acid bacteria (LAB), so it is necessary to apply starter cultures of selected strains. Traditionally, LAB starter cultures were applied to lower the pH by producing lactic acid and to inhibit the growth of undesirable epiphytic microorganisms by competing for nutrients. Nowadays, LAB inoculants have become an effective tool for creating microbial quality of silages by selecting species with extraordinary features. Epiphytic microflora characteristic of plant material used for the production of silages and the sources of undesirable microflora in the ensiling process are discussed. This review focuses on the most frequently studied issues related to the microbial silage quality and the recent trends in increasing the quality by LAB inoculants, with respect to recent directions for selecting types of modern LAB for inoculation. Among them, the main trends described were prevention of the growth of filamentous fungi and detoxification of mycotoxins by LAB inoculants, inhibition of yeast growth by LAB present in preparations and limiting the development of pathogenic bacterial microflora through controlled fermentation with the participation of LAB and the presence of their metabolites.

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Section: Undesirable Microflora and Its Sources In Plant Materials Intmentioning

confidence: 99%

Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a minireview

Fabiszewska

Zielińska

Wróbel

2019

World J Microbiol Biotechnol

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“…Some authors have suggested an inverse relationship between percentage adsorption and mycotoxin concentrations: when AF concentration increases, the adsorption percentage declines. This behaviour may be due to the saturation of the adsorption sites in the S. cerevisiae cell and not to a molecular structure modification of the toxin (Fernandes Oliveira et al 2013). Other researchers have suggested that increasing AFB 1 concentration in aqueous medium would not affect the percentage of AFB 1 binding.…”

Section: Discussionmentioning

confidence: 93%

Potentiation of the effect of a commercial animal feed additive mixed with different probiotic yeast strains on the adsorption of aflatoxin B₁

Poloni

Dogi

Pereyra

et al. 2015

Food Additives & Contaminants: Part A

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This study potentiates the adsorbent effect for aflatoxin B1 (AFB1) of a commercial additive (CA) of animal feed, containing inactive lysate of three Saccharomyces cerevisiae strains, active enzymes, adsorbents and a selenium-amino acid complex, when the additive was mixed separately with three S. cerevisiae strains. Levels of AFB1 of 20 and 50 ng g(-1) were used to determine the binding capacity of different concentrations of CA alone and in the presence of yeast strains, as well as toxin desorption, under gastrointestinal conditions. The viability of yeasts in the presence of CA was evaluated. The results show that the CA did not affect the viability of the yeast strains assayed. CA alone showed a low percentage adsorption. At 20 and at 50 ng g(-1), CA was highly efficient in adsorbing AFB1 when combined with RC016 and RC012 strains respectively. Desorption of AFB1 by CA alone and in combination with the yeasts increased with increasing levels of CA. The results demonstrate the improvement of CA in AFB1 adsorption once it is mixed with live yeasts.

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“…The stability of the LAB/aflatoxin complex in a wide range of pH is an important factor in the use of these microorganisms to remove aflatoxin from foods, once gastric release of the toxin would have negative health implications. Therefore, the complex formed has to resist environmental stress caused by the gastrointestinal tract, such as low pH and presence of bile (Oliveira, Bovo, Corassin, Jager, & Reddy, 2013).…”

Section: Discussionmentioning

confidence: 99%

The Ability of Lactobacillus rhamnosus in Solution, Spray-Dried or Lyophilized to Bind Aflatoxin B1

Campagnollo¹,

Franco²,

Rosim³

et al. 2014

JFR

Self Cite

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Aflatoxin B1 (AFB1) can cause carcinogenic, mutagenic, teratogenic and immunosuppressive effects in humans and animals. Several lactic acid bacteria species have the ability to bind AFB1 in vitro, showing a potential application for reducing the bioavailability of AFB1 in contaminated products. Thus, the aim of this study was to evaluate the capacity of Lactobacillus rhamnosus, non-viable and dried, in removing the AFB1 from a contaminated medium. L. rhamnosus were cultured in MRS broth, sterilized (121 ºC, 15 min.) to inactivate their metabolism and then dried by spray-drying or freeze-drying (lyophilization). Binding assays using AFB1 (1.0 µg/ml) and L. rhamnosus cells (1×1010 cells, in suspension or spray-dried or freeze-dried) were conducted at pH 3.0 and 6.0, room temperature and contact time of 60 min. Quantification of AFB1 was achieved by high performance liquid chromatography. Scanning electron microscope was also performed in order to analyze the drying effect on the atomized and lyophilized L. rhamnosus cells. For pH 3.0 and 6.0, there were no significant differences between AFB1 binding efficiency by L. rhamnosus cells in solution (45.9 ± 8.8% and 35.8 ± 7.7%, respectively) or freeze-dried (36.6 ± 7.1% and 27.2 ± 4.0%, respectively). However, the spray-dried cells lost completely the AFB1 binding capacity during atomization, which damaged the structural and functional properties of the bacterial cell wall. In conclusion, L. rhamnosus retained its AFB1 binding ability only when its cell wall remained intact as observed in the lyophilization procedure. Lyophilized L. rhamnosus cells therefore can be a practicable alternative for decontamination of food products susceptible to aflatoxin contamination.

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Recent Trends in Microbiological Decontamination of Aflatoxins in Foodstuffs

Cited by 12 publications

References 83 publications

Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a minireview

Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a minireview

Potentiation of the effect of a commercial animal feed additive mixed with different probiotic yeast strains on the adsorption of aflatoxin B₁

The Ability of Lactobacillus rhamnosus in Solution, Spray-Dried or Lyophilized to Bind Aflatoxin B1

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Recent Trends in Microbiological Decontamination of Aflatoxins in Foodstuffs

Cited by 12 publications

References 83 publications

Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a minireview

Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a minireview

Potentiation of the effect of a commercial animal feed additive mixed with different probiotic yeast strains on the adsorption of aflatoxin B1

The Ability of Lactobacillus rhamnosus in Solution, Spray-Dried or Lyophilized to Bind Aflatoxin B1

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Product

Resources

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Potentiation of the effect of a commercial animal feed additive mixed with different probiotic yeast strains on the adsorption of aflatoxin B₁