Nasopharyngeal Gram-negative bacilli colonization in Brazilian children attending day-care centers

Lima, Ana Beatriz Mori; Leão, Lara Stefânia Netto de Oliveira; Oliveira, Luciana Silva da Cruz; Pimenta, Fabiana Cristina

doi:10.1590/s1517-83822010000100005

Cited by 15 publications

(20 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pathogens which commonly colonize the upper airways include Streptococcus pneumoniae (the pneumococcus), Staphylococcus aureus, Haemophilus influenzae, Neisseria meningitidis, and several Gram-negative bacilli [5][6][7]. The pneumococcus and H. influenzae are among the leading causes of bacterial respiratory tract infections in young children [8,9].…”

Section: Introductionmentioning

confidence: 99%

Longitudinal changes in the nasopharyngeal resistome of South African infants using shotgun metagenomic sequencing

et al. 2020

View full text Add to dashboard Cite

Introduction Nasopharyngeal (NP) colonization with antimicrobial-resistant bacteria is a global public health concern. Antimicrobial-resistance (AMR) genes carried by the resident NP microbiota may serve as a reservoir for transfer of resistance elements to opportunistic pathogens. Little is known about the NP antibiotic resistome. This study longitudinally investigated the composition of the NP antibiotic resistome in Streptococcus-enriched samples in a South African birth cohort. Methods As a proof of concept study, 196 longitudinal NP samples were retrieved from a subset of 23 infants enrolled as part of broader birth cohort study. These were selected on the basis of changes in serotype and antibiogram over time. NP samples underwent short-term enrichment for streptococci prior to total nucleic acid extraction and whole metagenome shotgun sequencing (WMGS). Reads were assembled and aligned to pneumococcal reference genomes for the extraction of streptococcal and non-streptococcal bacterial reads. Contigs were aligned to the Antibiotic Resistance Gene-ANNOTation database of acquired AMR genes. Results AMR genes were detected in 64% (125/196) of the samples. A total of 329 AMR genes were detected, including 36 non-redundant genes, ranging from 1 to 14 genes per sample. The predominant AMR genes detected encoded resistance mechanisms to beta-lactam (52%, 172/329), macrolide-lincosamide-streptogramin (17%, 56/329), and tetracycline antibiotics

show abstract

Section: Introductionmentioning

confidence: 99%

Longitudinal changes in the nasopharyngeal resistome of South African infants using shotgun metagenomic sequencing

et al. 2020

View full text Add to dashboard Cite

show abstract

“…According to a recent release by the Sickle Cell Foundation of Nigeria, one out of four Nigerians carries the sickle cell gene compared with the United States of America where one in twelve of African Americans carry the sickle cell gene [1]. It has been shown nasopharyngeal bacterial colonization is common in the young infants which often precedes development of invasive diseases [2]. Knowledge of nasopharyngeal bacteria colonization in children is desirable because the nasopharynx harbors potential pathogens.…”

Section: Introductionmentioning

confidence: 99%

“…A recent study of the nasopharyngeal of S. pneumoniae carriage among rural Gambian in West Africa sub-region, showed the nasopharynx carriage of Gambia infants by S. pneumoniae is rapid and highly dynamic, rising from prevalence of 1.5% at birth to 77% at 2 months, 5 months 86% and to 78% at 12 months [7]. A Brazilian study reported the nasopharynx may constitute an ecological reservoir or source of dissemination of S. pneumoniae, H. influenzae, S. aureus and other Gram-negative bacilli (GNB) [2]. Infants and children are the most vulnerable to opportunistic infections mainly because of their relatively immature immune system besides the elderly and the immunocompromised.…”

Section: Introductionmentioning

confidence: 99%

Characterization of bacterial isolates cultured from the nasopharynx of children with sickle cell disease (SCD)

et al. 2015

View full text Add to dashboard Cite

Background: We characterized bacterial isolates from the nasopharynx of 84 Sickle cell disease patients 78 of whom were HbSS and 6 HbSC aged 4 four months to 15 years at Wesley Guild Hospital, Ilesa, southwestern Nigeria between February-September, 2014. Method: Sterile cotton-tipped initially dipped into sterile saline thereafter was introduced into nasopharynx of each patient and thereafter applied onto sterile thioglycolate medium and incubated at 370 C for 24 hr, When growth was noticed, samples were inoculated onto enriched, selective and differential bacteriologic media. Bacterial colonies that grew on such media were picked and characterized by Grams' reaction, cultural, morphologic and biochemical methods. Antibiotic sensitivity tests were determined by the disc diffusion method. Demographic data relating to severity of SCD were provided. Results: Altogether, 119 isolates were cultured from the nasopharynx. Gram positive bacteria predominated (65.54%) and Corynebacterium spp (44.53%) dominated comprising of 19 (35.84%) Corynebacterium xerosis 11 (20.75%) Corynebacterium diphtheriae, 10 (18.86) Corynebacterium pseudodiphtheriticum, 8 (15.09%) Corynebacterium ulcerans, 3 (6.66%) Corynebacterium spp and 2 (3.77%) Corynebacterium jeikeium. Other Gram positive rods cultured were Arcanobacterium haemolyticum 6 (5%). Bacillus subtilis was 3 (2.5%), Actinomyces isrealli 3 (2.5%) and Norcadia asteroids 1 (0.84%). Low frequency of nasopharyngeal colonization recorded for Haemophilus influenzae 4.2%, S. pneumoniae 2.5% and S. aureus 4 (3.36%) and multiresistance was widespread for most isolates.

show abstract

“…Entretanto, além das mãos, a colonização de outros sítios anatômicos por agentes infecciosos também contribui para a transmissão cruzada de patógenos (3,(7)(8)(9) . (6,(14)(15) .…”

Section: Introductionunclassified

“…A adesão às medidas de precaução padrão por trabalhadores de saúde destaca-se como estratégia primordial para o controle dos agravos, assegurando a qualidade da assistência prestada ao cliente, bem como a integridade do profissional (2,13) . (8,17) .…”

Section: Introductionunclassified

Prevalência de bastonetes Gram-negativos isolados da saliva de trabalhadores da saúde

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

Nasopharyngeal Gram-negative bacilli colonization in Brazilian children attending day-care centers

Cited by 15 publications

References 11 publications

Longitudinal changes in the nasopharyngeal resistome of South African infants using shotgun metagenomic sequencing

Longitudinal changes in the nasopharyngeal resistome of South African infants using shotgun metagenomic sequencing

Characterization of bacterial isolates cultured from the nasopharynx of children with sickle cell disease (SCD)

Prevalência de bastonetes Gram-negativos isolados da saliva de trabalhadores da saúde

Contact Info

Product

Resources

About