Characterization of recombinant MSP5 Anaplasma marginale Havana isolate

Corona, Belkis; Machado, Humberto; Rodríguez, Mayra G; Martínez, Siomara

doi:10.1590/s1517-83822009000400032

Cited by 6 publications

(1 citation statement)

References 21 publications

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“…In the prediction, we found that all 36 UdLAMPs have conformational and linear epitopes for B lymphocytes and are predicted as antigenic (VaxiJen predictor). A considerable number of regions of conformational and linear epitopes were greater than or equal to the values for Msp5, one of the main surface proteins of A. marginale, known for its ability to induce antibody production during cattle infection [14]. The presence of these epitopes points to these molecules as agents capable of stimulating the development of a humoral immunological response.…”

Section: Discussionmentioning

confidence: 99%

Immunoinformatics and analysis of antigen distribution of Ureaplasma diversum strains isolated from different Brazilian states

Santos

Neves

et al. 2020

BMC Vet Res

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Background Ureaplasma diversum has numerous virulence factors that contribute to pathogenesis in cattle, including Lipid-associated membrane proteins (LAMPs). Therefore, the objectives of this study were to evaluate in silico important characteristics for immunobiological applications and for heterologous expression of 36 LAMPs of U. diversum (UdLAMPs) and, also, to verify by conventional PCR the distribution of these antigens in strains of Brazilian states (Bahia, Minas Gerais, São Paulo, and Mato Grosso do Sul). The Manatee database was used to obtain the gene and peptide sequences of the antigens. Similarity and identity studies were performed using BLASTp and direct antigenicity was evaluated by the VaxiJen v2.0 server. Epitope prediction for B lymphocytes was performed on the BepiPred v2.0 and CBTOPE v1.0 servers. NetBoLApan v1.0 was used to predict CD8+ T lymphocyte epitopes. Subcellular location and presence of transmembrane regions were verified by the software PSORTb v3.0.2 and TMHMM v2.2 respectively. SignalP v5.0, SecretomeP v2.0, and DOLOP servers were used to predict the extracellular excretion signal. Physico-chemical properties were evaluated by the web-software ProtParam, Solpro, and Protein-sol. Results In silico analysis revealed that many UdLAMPs have desirable properties for immunobiological applications and heterologous expression. The proteins gudiv_61, gudiv_103, gudiv_517, and gudiv_681 were most promising. Strains from the 4 states were PCR positive for antigens predicted with immunogenic and/or with good characteristics for expression in a heterologous system. Conclusion These works contribute to a better understanding of the immunobiological properties of the UdLAMPs and provide a profile of the distribution of these antigens in different Brazilian states.

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Section: Discussionmentioning

confidence: 99%