In vitro degradation of linamarin by microorganisms isolated from cassava wastewater treatment lagoons

Vasconcellos, S. P; Cereda, M. P.; Cagnon, J. R.; Foglio, M.A.; Rodrigues, R.A.; Manfio, G. P.; Oliveira, V. M.

doi:10.1590/s1517-83822009000400019

Cited by 16 publications

(13 citation statements)

References 8 publications

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“…In this study, at the end of incubation period of day 12, the strainWOB3 was able to degrade linamarin from an initial concentration of 137.18 to final concentration of 26.73 mg/L (80.52 %) while strain WOB7 was able to degrade linamarin from concentration of 135.90 mg/L to final concentration of 29.79 mg/L (78.08 %). In similar study by Vasconcellos et al (2009), the test organism was able to degrade linamarin from an initial concentration of 7.47 mg/mL to final concentration of 2.16 mg/mL (71 %) after seven days. Murugan et al (2012) reported the utilization of cyanogenic glycoside by Bacillus subtilis KM05 which went through assimilatory degradation with the release of hydrogen cyanide and ammonia.…”

Section: Hplc Analysis Of the Products Of Degradation Of Linamarin Bymentioning

confidence: 64%

“…4). DISCUSSION Some studies on cyanoglucoside degradation by Candida tropicalis and Candida utilis, exist in literature but these studies did not report the degradation rates (Legras et al, 1990;Fagbemi and Ijah, 2006;Vasconcellos et al, 2009). To the best of our knowledge, this was first time where other products of linamarin degradation were detected using high performance liquid chromatography (HPLC).…”

Section: Hplc Analysis Of the Products Of Degradation Of Linamarin Bymentioning

confidence: 90%

“…Cassava (Manihot esculenta Crantz) roots and leaves contain high concentrations of linamarin (alphahydroxyisobutyronitrile-beta-D-glucopyranoside) and lotaustralin (methyllinamarin) (Vasconcellos et al, 2009). Linamarin is the most abundant cyanoglucoside present in cassava cells (Conn, 1969) and may generate the equivalent to 0.2-100 mg of HCN per 100 g of fresh cassava following cellular lysis (Bradbury et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

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In vitro Degradation of Extracted Cassava Linamarin by Bacillus Species Isolated from cassava wastewater

Ogunyemi

Samuel

Buraimoh

et al. 2017

Egyptian Academic Journal of Biological Sciences, G. Microbiolo

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Linamarin is the most abundant cyanoglucoside present in cassava cells and may generate the equivalent amount of hydrocyanic acid. This study was aimed to assess degradative capacities of Bacillus pumilus strain WOB3 and WOB7on linamarin. The test organisms for linamarase activity were identified on the basis of phenotype, biochemical properties and 16S rDNA gene sequencing as: Bacillus pumilus strain WOB3 KX774195 and Bacillus pumilus strain WOB7 KX774196. Growth studies showed that the strains grew in all the substrates tested. The doubling times of Bacillus pumilus strain WOB3 and Bacillus pumilus strain WOB7 were 8.25 d and 7.53 d on cassava effluent, 6.30 d and 5.78 d on supplemented cassava effluent, 8.66 d and 9.90 d on waste leachate and 6.30 d and 9.24 d on supplemented waste leachate respectively; with specific growth rates of 0.084 d-1 and 0.092 d-1 on cassava effluent, 0.11 d-1 and 0.12 d-1 on supplemented cassava effluent, 0.080 d-1 and 0.070 d-1 on waste leachate and 0.11 d-1 and 0.075 d-1 on supplemented waste leachate respectively. High-performance liquid chromatographic analysis studies revealed that linamarin degradation by the strains followed a linamarase pathway involving CO 2 and HCN as metabolic intermediates. Based on HPLC analysis, linamarin residual concentration at day 12 by the strains WOB3 and WOB7 was 26.73 mgL-1 (19.79 %) and 29.79 mgL-1 (21.92 %). These novel features make the bacteria suitable candidates for in-situ application on sites contaminated with cassava processing wastes.

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Section: Hplc Analysis Of the Products Of Degradation Of Linamarin Bymentioning

confidence: 64%

Section: Hplc Analysis Of the Products Of Degradation Of Linamarin Bymentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

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In vitro Degradation of Extracted Cassava Linamarin by Bacillus Species Isolated from cassava wastewater

Ogunyemi

Samuel

Buraimoh

et al. 2017

Egyptian Academic Journal of Biological Sciences, G. Microbiolo

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“…Agro-industrial wastes provide the carbon and nitrogen sources and other elements needed to carry out microbial metabolism (Mata-Gómez et al, 2014). Cassava (Manihot esculenta) residues, like cassava wastewater (manipueira), contain a small amounts of starch, protein, cellulose and other nutrients (Nitschke and Pastore, 2004;Choubert et al, 2005;Kaewpintong et al, 2006;Vasconcellos et al, 2009;Casullo et al, 2010;Yang et al, 2011;Yimyoo et al, 2011, Saoudi andFei, 2012). The liquid waste from industrial cassava processing contains a large number of pollutants and has a significant adverse environmental impact.…”

Section: Introductionmentioning

confidence: 99%

Untitled

2015

IJAPR

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Astaxanthin is a xanthophyll, a very common red-colored pigment of marine organisms that are in increasing demand in the market because of their applications in the food, cosmetics, and pharmaceutical industries. Particularly, astaxanthin has been found to play protective roles against many chronic human diseases, and diseases associated with oxidative stresses caused by excess free radicals and other reactive oxygen species. Mucor circinelloides f. circinelloides is able to accumulate astaxanthin by stress conditions. The aim of this study was to produce astaxanthin by M. circinelloides f. circinelloides cultivated in the presence of exogenous stress conditions using a natural medium of cassava wastewater (CWW), continuous illumination with blue LED and fluorescent lamps in a bioreactor for 96 hours. The best condition for astaxanthin was under a fluorescent lamp (150 µg/g per dry biomass). The carotenoid fraction was extracted using acetone, followed by separation and purification by thin-layer chromatography. The astaxanthin was identified and quantified using spectrophotometry, based on the absorption coefficients. The antioxidant activity was evaluated using (2,2-Diphenyl-1-picrylhydrazyl) DPPH, and a higher antioxidant effect was observed by fluorescent lamp. This is one of the first reports on antioxidant activity in astaxanthin produced by Mucor circinelloides and the biotechnological potential for this fungus was demonstrated.

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“…Some researchers had successfully isolated some microorganisms such as lactic acid bacteria, yeast and moulds, which could produce effective exogenous β-glucosidase. These microorganisms were added to CGs-containing samples and completely degraded the CGs by fermentation (Lei et al, 1999;Vasconcellos et al, 2009). However, it had been reported that bioactive ingredient (lignan) in flaxseed could be destructed under acidic condition of the pH Yuan et al, 2008), which may occur due to the metabolism action of microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Construction of recombinant Pichia strain GS115-Ch-Glu expressing β-glucosidase and cyanide hydratase for cyanogenic glycosides detoxification

Wu¹

2012

Afr. J. Biotechnol.

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A recombinant Pichia strain GS115-Ch-Glu expressing both β-glucosidase and cyanide hydratase was constructed to remove cyanogenic glycosides in edible plants. The β-glucosidase could hydrolyze cyanogenic glycosides into cyanide and its gene (Glu) was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR). A cyanide hydratase could catalyze cyanide to formamide and its gene (Ch) was obtained by C (PCR). Both Glu and Ch genes were integrated into the genome of Pichia strain GS115 by homologous recombination. The engineering strain GS115-Ch-Glu was confirmed by multiple analytical methods, and was inoculated to induce expression of the recombinant Glu and Ch genes. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed that the fused proteins of interest had specifically been expressed as 31 and 52 KDa, which corresponded to the predicted molecular weights of the two enzymes. The enzyme preparation containing cyanide hydratase and β-glucosidase produced by strain GS115-Ch-Glu could completely decompose cyanogenic glycosides in flaxseed power into cyanide, and further catalyze the hydrolysis of over 80% cyanide.

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In vitro degradation of linamarin by microorganisms isolated from cassava wastewater treatment lagoons

Cited by 16 publications

References 8 publications

In vitro Degradation of Extracted Cassava Linamarin by Bacillus Species Isolated from cassava wastewater

In vitro Degradation of Extracted Cassava Linamarin by Bacillus Species Isolated from cassava wastewater

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Construction of recombinant Pichia strain GS115-Ch-Glu expressing β-glucosidase and cyanide hydratase for cyanogenic glycosides detoxification

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