Molecular epidemiology of Shigella spp strains isolated in two different metropolitam areas of southeast Brazil

Angelini, Michelle; Stehling, Eliana Guedes; Moretti, Maria Luíza; Silveira, Wanderley Dias da

doi:10.1590/s1517-83822009000300034

Cited by 9 publications

(12 citation statements)

References 18 publications

(13 reference statements)

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“…In developing countries with tropical climates, Shigella infection is common throughout the year, but higher isolation rates are found during the summer and rainy seasons (Angelini et al . ; Ashkenazi and Cohen ).…”

Section: Epidemiologymentioning

confidence: 98%

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Update on:Shigellanew serogroups/serotypes and their antimicrobial resistance

Sethuvel

Ragupathi

Anandan

et al. 2016

Lett Appl Microbiol

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Shigellosis represents a major burden of disease in developing countries. A low infectious dose allows the disease to be spread effectively. Although shigellosis is mostly a self‐limiting disease, antibiotics are recommended to reduce deaths, disease symptoms and organism‐shedding time. However, in India, antimicrobial resistance among the genus Shigella is more common than among any other enteric bacteria. Notably, new serotypes or subserotypes in Shigella are reported from various parts of the world. Identification of new subserotypes of Shigella spp. is becoming a major issue as these strains are nontypeable by conventional serotyping. The commercially available antisera may not cover all possible epitopes of the O lipopolysaccharide antigen of Shigella serotypes. Therefore, molecular methods which most closely approach the resolution of full serotyping are necessary to identify such strains. In addition, the knowledge of a prevalent serotype in various geographic regions may assist in formulating strategies such as the development of a vaccine to prevent infection especially when the immunity to disease is serotype specific, and to understand the disease burden caused by new Shigella serotypes.

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Section: Epidemiologymentioning

confidence: 98%

“…Seasonality of infection has changed from a peak in summer to a higher incidence in the late summer and autumn. In developing countries with tropical climates, Shigella infection is common throughout the year, but higher isolation rates are found during the summer and rainy seasons (Angelini et al 2009;Ashkenazi and Cohen 2013).…”

Section: Epidemiologymentioning

confidence: 99%

Update on:Shigellanew serogroups/serotypes and their antimicrobial resistance

Sethuvel

Ragupathi

Anandan

et al. 2016

Lett Appl Microbiol

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“…Shigella spp. é um importante agente causador de diarreia e disenteria no mundo todo (ANGELINI et al, 2009;ANDERS et al, 2015;KAHSAY e TEKLEMARIAM, 2015).…”

Section: Tabela 9 -unclassified

“…A incidência da shigelose geralmente varia de acordo com a idade do indivíduo e com o índice de desenvolvimento humano (IDH). Crianças menores de cinco anos são as mais afetadas por essa doença, sendo que Shigella é altamente infecciosa mesmo em concentrações baixas (ANGELINI et al, 2009).…”

Section: De Acordo Com O Centers For Disease Control and Prevention (unclassified

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Análise do potencial patogênico, diversidade genotípica e perfil de resistência de linhagens de Shigella sonnei isoladas de 1983 a 2014 no Estado de São Paulo

Seribelli¹

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≥74,7% e 27 dessas linhagens exibiram uma similaridade ≥83,0%. Similarmente, o dendrograma gerado pelo ERIC-PCR agrupou as 72 linhagens de S. sonnei em dois clusters designados ERIC-A e ERIC-B. O cluster ERIC-A agrupou 37 linhagens isoladas entre 1983-2014 que exibiram uma similaridade ≥78,8% e mais especificamente 36 dessas linhagens apresentaram uma similaridade ≥82,3%. O cluster ERIC-B agrupou 34 linhagens de S. sonnei isoladas entre 1987-2014 que exibiram uma similaridade ≥84,0%. Também por MLVA as linhagens foram agrupadas em dois clusters designados MLVA-A e MLVA-B. O cluster MLVA-A agrupou 31 linhagens isoladas entre 1983-2014 com uma similaridade ≥40%. O cluster MLVA-B agrupou 41 linhagens isoladas entre 1983-2014 com uma similaridade ≥21,6%. Todas as 20 S. sonnei foram tipadas por MLST como ST152. Conclui-se que o potencial patogênico das linhagens estudadas foi destacado pela presença de importantes genes de virulência. A alta porcentagem de resistência para alguns antimicrobianos testados, tais como, sulfametoxazol-trimetoprim e tetraciclina é preocupante e pode levar à falha terapêutica. Os resultados da tipagem molecular sugerem que existam dois subtipos prevalentes nas linhagens de S. sonnei estudadas que se diferenciaram pouco geneticamente e contaminaram humanos durante 31 anos na região metropolitana de Ribeirão Preto no Estado de São Paulo. O resultado do MLST indica que as linhagens estudadas de Shigella sonnei isoladas no Brasil descendem de um precursor comum.

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A high number of multidrug-resistant and predominant genetically related cluster of Shigella flexneri strains isolated over 34 years in Brazil

et al. 2020

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Shigella flexneri has been a major public health problem in developing countries. This work analyzed the frequency of 16 virulence genes, the genotypic diversity, and the antimicrobial resistance profiles of 130 S. flexneri strains isolated in Brazil. The ipaH gene was found in all the 130 strains. The frequencies of the other genes were variable ial (88.5%), sigA (82.3%), iuc (74.6%), virA (73%), pic (72.3%), virF (57.7%), sat (48.5%), ipaBCD (37%), sen (36%), set1A (35.4%), sepA (30%), set1B (30%), virB (14%), icsA (10%), and ipgD (5.4%). A total of 57 (43.8%) strains were multidrug-resistant. ERIC-PCR grouped 96 of the strains into a single cluster with ≥ 70.4% of similarity, 75 of these strains presented a similarity ≥ 80.9%. PFGE grouped 120 of the strains into a single cluster with 57.4% of similarity and 82 of these strains presented a similarity ≥ 70.6%. In conclusion, the high frequency of some virulence genes reinforces the pathogenic potential of the strains studied. The high rates of MDR strains are alarming once it may lead to failure when antimicrobial treatment is necessary. Genotype techniques reveled a major cluster with high genetic similarity including S. flexneri strains from the different Brazilian states and distinct years of isolation, showing that they probably emerged from a common ancestor.

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Molecular epidemiology of Shigella spp strains isolated in two different metropolitam areas of southeast Brazil

Cited by 9 publications

References 18 publications

Update on:Shigellanew serogroups/serotypes and their antimicrobial resistance

Update on:Shigellanew serogroups/serotypes and their antimicrobial resistance

Análise do potencial patogênico, diversidade genotípica e perfil de resistência de linhagens de Shigella sonnei isoladas de 1983 a 2014 no Estado de São Paulo

A high number of multidrug-resistant and predominant genetically related cluster of Shigella flexneri strains isolated over 34 years in Brazil

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