Adherence assays and Slime production of Vibrio alginolyticus and Vibrio parahaemolyticus

Abdallah, Fethi Ben; Chaieb, Kamel; Zmantar, Tarek; Kallel, Héla; Bakhrouf, Amina

doi:10.1590/s1517-83822009000200033

Cited by 41 publications

(21 citation statements)

References 28 publications

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“…The slime was produced by 100% of the V. alginolyticus, V. diabolicus, V. anguillarum and V. aestuarianus isolates , but was not detected in V. parahaemolyticus and V. fluvialis . In line with this, Ben Abdallah, Chaieb, Zmantar, Kallel, and Bakhrouf () reported that fish‐borne V. alginolyticus were producing the slime, but the V. parahaemolyticus were not. It has been known that the ability of Vibrio spp.…”

Section: Resultsmentioning

confidence: 67%

Vibrio spp. from Yesso scallop (Patinopecten yessoensis) demonstrating virulence properties and antimicrobial resistance

Silva

Hossain

Dahanayake

et al. 2019

Journal of Food Safety

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We report the prevalence and characterization of Vibrio spp. isolated from marketed Yesso scallop (Patinopecten yessoensis) in Korea. A total of 30 isolates including, V. parahaemolyticus (n = 2), V. alginolyticus (n = 9), V. fluvialis (n = 7), V. diabolicus (n = 7), V. anguillarum (n = 4) and V. aestuarianus (n = 1) were isolated and identified. The phenotypic pathogenicity tests demonstrated that, 18 (60%), 21 (70%), 18 (60%), 7 (23%), 22 (73%), 21 (70%), 9 (30%), and 11 (33%) of the isolates were positive for DNase, protease, gelatinase, lipase, phospho‐lipase, amylase, slime production, and haemolysis, respectively. PCR assays revealed the prevalence of toxR, tlh, VAC, vfh, hupO, and VPI genes among the isolates with varying combinations. A close genetic affinity among V. alginolyticus and V. diabolicus strains was observed. Also the virulence genes specific to one Vibrio species were detected among other species as well. In addition, 29/30 (97%) isolates were multidrug resistant, while higher resistance rates were shown for ampicillin, colistin, vancomycin, and cephalothin. The results imply that the scallops in Korean markets harbor Vibrio spp., which are potentially virulent and multidrug resistant, thus their public health implications should not be underrated. Practical applications For many decades, vibrios are known for its importance in seafoodborne illnesses. Yesso scallop is the most popular and extensively cultured scallop variety in Korea. Therefore, we sought to assess the marketed fresh Yesso scallops for the prevalence and molecular characterization of Vibrio species. A total of 30 strains were isolated and identified by a series of biochemical tests, subsequent gyrB gene sequencing and phylogenetic analyses. Six Vibrio spp. were identified with V. alginolyticus as the most prevalent. Interestingly, V. alginolyticus was genetically similar to V. diabolicus. Besides, the virulence genes specific to V. alginolyticus and V. parahaemolyticus were observed in other species as well. It suggests that the detection of the species‐specific genes does not ensure the correct identification of pathogenic vibrios. Further, the occurrence of V. parahaemolyticus‐specific virulence genes in other Vibrio spp. potentially complicates the correct tracking of V. parahaemolyticus infections. In addition, 73% of these Vibrio spp. isolates showed multiple antibiotic resistance (MAR) indices higher than 0.2, which signifies their high risk of infection. Collectively, these results provide important evidence that not only the well‐known pathogenic vibrios like V. parahaemolyticus, but also other Vibrio spp. can act alike because of their similar characteristics.

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Section: Resultsmentioning

confidence: 67%

Vibrio spp. from Yesso scallop (Patinopecten yessoensis) demonstrating virulence properties and antimicrobial resistance

Silva

Hossain

Dahanayake

et al. 2019

Journal of Food Safety

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“…Being a halophilic organism, Vibrio is the major microbial constituent in the brackishwater system. Higher microbial load noticed over submerged substrates indicates biofilm forming microbes which secrete extracellular polymeric substances (Abdallah, Chaieb, Zmantar, Kallel, & Bakhrouf, ; Sharma et al, ) which in turn results in strong immune enhancing ability in shrimp P. monodon ( Sharma et al, ). Therefore, it could be concluded that a higher level of beneficial microbes over the bamboo substratum enhanced the immune system of shrimps.…”

Section: Discussionmentioning

confidence: 99%

Integration of substrate in biofloc based system: Effects on growth performance, water quality and immune responses in black tiger shrimp, Penaeus monodon culture

Kumar

Anand

et al. 2019

Aquac Res

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To evaluate effect of substrate integration in biofloc based system, a 52‐day growth experiment was conducted using black tiger shrimp, Penaeus monodon juveniles (3.32 ± 0.07 g). The factorial design consisted of floc, F (with or without) as first factor and substrate (bamboo mat, B; nylon mesh, N; and without substrate) as second factor. This resulted six treatments; F + B, F + N, F, B, N and a control without biofloc and substrate. Shrimps were stocked at 110 nos. m–3 in Fibre Reinforced Plastic (FRP) tanks and, rice flour was used as carbon source in biofloc based treatments. Incorporation of nylon mesh and bamboo mat in biofloc system trapped 31.3%–38.6% and 8.5%–13.5% total suspended solids respectively and reduced bottom solid deposition. Among the substrate based groups, significantly better development of biofilm with higher microbial population noticed in F + B compared with nylon mesh. Similarly, significantly higher final growth (p < 0.01) was recorded in F + B system followed by F + N while no significant difference in body weight recorded among floc, F or substrate based groups (B, N). Biofloc and substrate integration (F + B and F + N) resulted significantly (p < 0.01) lower feed conversion ratio compared to control and floc. Incoporation of bamboo substrate in biofloc, (F + B) improved shrimp immune responses through higher hemocyte counts and prophenoloxidase activity compared to other treatments. The study revealed that integration of substrate in the biofloc system improved growth performance, FCR and immune parameters in shrimp by trapping the suspended biofloc particles, better water quality parameters, enhanced biofilm growth and provision of quality natural food.

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“…The average OD values along with standard deviations were calculated for further analysis. Biofilm formation was interpreted as positive for cases where OD 570 >0.1 or negative for cases where OD 570 <0.1 (Abdallah et al, 2009).…”

Section: Biofilm-forming Ability On Microtitration Platementioning

confidence: 99%

The prevention and removal of biofilm formation ofStaphylococcus aureusstrains isolated from raw milk samples by citric acid treatments

Akbaş

Kökümer

2015

Int J of Food Sci Tech

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In this study, the antibiofilm activity of citric acid treatment on Staphylococcus aureus strains isolated from raw milk samples was evaluated. For this purpose, the prevention and removal of biofilm formation of S. aureus strains by citric acid treatments (2% and 10%) for 20 min were investigated for comparison with peracetic acid treatment (0.3%) on both microtitration plate and stainless steel coupons. The results indicated that the prevention and removal of biofilm formation and the numbers of prevented or removed S. aureus strains using citric acid treatments were observed to be higher than those using peracetic acid treatment on both surfaces. The prevention and removal of biofilm formation were substantially higher when the concentration of citric acid treatment increased from 2% to 10% and the stainless coupons were used. The results show that citric acid can be used as an alternative disinfectant in controlling biofilm formation in the dairy industry.

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Adherence assays and Slime production of Vibrio alginolyticus and Vibrio parahaemolyticus

Cited by 41 publications

References 28 publications

Vibrio spp. from Yesso scallop (Patinopecten yessoensis) demonstrating virulence properties and antimicrobial resistance

Vibrio spp. from Yesso scallop (Patinopecten yessoensis) demonstrating virulence properties and antimicrobial resistance

Integration of substrate in biofloc based system: Effects on growth performance, water quality and immune responses in black tiger shrimp, Penaeus monodon culture

The prevention and removal of biofilm formation ofStaphylococcus aureusstrains isolated from raw milk samples by citric acid treatments

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