Production of actinomycin-D by the mutant of a new isolate of Streptomyces sindenensis

Abstract

An actinomycin-D producing strain was isolated from soil and characterized as Streptomyces sindenensis. The culture was subjected to UV irradiation and a mutant with 400% higher actinomycin-D production was isolated (400 mg/l -1 as compared to 80 mg/l -1 produced by the parent). Production medium was optimized and antibiotic yield with the mutant was enhanced to 850 mg/l -1 which is 963% higher as compared with the parent.

Optimization of Culture Conditions for Production of Bioactive Metabolites by <i>Streptomyces</i> spp. Isolated from Soil

“…Peptone followed by casein has been observed to be the best nitrogen sources by Gopi Reddy et al, on their studies on S. rochei [11]. Peptone has been found to favour antibiotic production by other authors too [28] [29].…”

Antimicrobial activity of actinomycetes and characterization of actinomycin-producing strain KRG-1 isolated from Karoo, South Africa

“…The actinomycines belong to a family of chromopeptide lactone antibiotics that present antitumor and cytotoxic properties (Praveen et al, 2008). They represent an important class of natural products that, despite being discovered more than 70 years ago, continue to be a focus of many research areas, especially in the biological and medicinal sciences (Kurosawa et al, 2006).…”

Identification and characterization ofStreptomyces flavogriseusNJ-4 as a novel producer of actinomycin D and holomycin

“…parvulus (152 mg/l actinomycin D under optimized conditions) (Praveen, Tripathi & Bihari, 2008), a mutant strain of S . sindenensis (850 mg/l actinomycin D) (Praveen et al, 2008) and S . avermitilis (1,770 mg/l actinomycin) (Chen et al, 2012).…”

“…This compound has also been recommended for the treatment of acquired immunodeficiency syndrome based on the ability of actinomycin D to inhibit human immunodeficiency virus minus-strand transfer and insertion into double helical DNA, thereby inhibiting the activity of the DNA-dependent RNA polymerase (Rill & Hecker, 1996). Although investigation has slowed recently, there are still some studies that focus on relevant issues of production, such as the yield, environment (Rebecca & Peter, 2014) and optimum fermentation conditions (Praveen, Tripathi & Bihari, 2008; Praveen et al, 2008). This continued study indicates that it not only has traditional clinical value but also has significance in continuing academic research.…”