Identification and Molecular Characterization of Norovirus in São Paulo State, Brazil

Morillo, Simone Guadagnucci; Cilli, Audrey; Carmona, Rita de Cássia Compagnoli; Timenetsky, María do Carmo Sampaio Tavares

doi:10.1590/s1517-83822008000400004

Cited by 18 publications

(15 citation statements)

References 28 publications

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“…Este estudo revelou uma preponderância do GII, confirmando que esse genogrupo específico é o mais frequentemente associado a doenças causadas por NoV em todo o mundo, em comparação com o GI 12,14 . As consequências epidemiológicas desse achado ainda não estão claras; estudos sobre as possíveis diferenças nas propriedades biológicas, tais como virulência, vias de transmissão, estabilidade do vírus no ambiente devem fornecer explicações sobre o tema 14 .…”

Section: Discussionunclassified

“…Embora o NoV tenha sido o primeiro vírus claramente associado à gastroenterite aguda, a impossibilidade de cultivá-lo subestimou a importância da infecção por NoV em gastroenterite aguda, e dificultou a realização de estudos epidemiológicos. Dessa forma, a importância do NoV como agente etiológico da gastroenterite aguda tanto em casos esporádicos como em surtos só pôde ser estabelecida após o desenvolvimento de métodos moleculares para a sua detecção 12,13 .…”

Section: Introductionunclassified

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Characterization of rotavirus and norovirus strains: a 6-year study (2004-2009)

et al. 2011

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Objective: To monitor rotavirus (RV) and norovirus (NoV) infections in hospitalized children ≤ 5 years with acute gastroenteritis in the state of São Paulo, Brazil, during a 6-year period (2004)(2005)(2006)(2007)(2008)(2009).Methods: This retrospective study was conducted with 61 medical centers with convenient surveillance fecal specimens, investigated by enzyme-linked immunosorbent assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis, reverse polymerase chain reaction and sequencing to genotype characterization.Results: RV and NoV infections were detected in 29.6% (144∕487) and 29.2% (26/89) of the samples, respectively. The most frequent RV genotypes detected were G9P [8] Métodos: Este estudo retrospectivo foi realizado em 61 centros médicos, utilizando amostras fecais coletadas por conveniência, analisadas por ensaio imunoenzimático, eletroforese em gel de poliacrilamida, transcrição reversa seguida de reação em cadeia pela polimerase e sequenciamento para caracterização dos genótipos. Conclusões:A distribuição dos genótipos de RV variou com os anos, acompanhada pela redução no número de casos detectados. Ė necessário intensificar a vigilância pós-implantação da vacina contra RV, visando monitorar as cepas circulantes e sua eficácia contra possíveis genótipos emergentes. Os NoVs têm sido cada vez mais identificados como agentes etiológicos relevantes entre crianças hospitalizadas e exercem um papel importante na etiologia viral da gastroenterite pediátrica aguda no estado de São Paulo. Artigo submetido em 07.04.11, aceito em 15.06.11. J Pediatr (Rio J)

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Section: Discussionunclassified

Section: Introductionunclassified

Characterization of rotavirus and norovirus strains: a 6-year study (2004-2009)

et al. 2011

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“…In England, distinct prevalence rates have been reported for NoV infection among elderly inpatients, ranging from 0.3% [28] to 14.9% [23]. Previous studies conducted in different Brazilian states showed that the prevalence of NoV among hospitalized children ranges from 9% to 36% [29,30].…”

Section: Discussionmentioning

confidence: 99%

Detecção de rotavírus e norovírus na população idosa na cidade de Caxias do Sul, Rio Grande do Sul, Brasil, de 2010 a 2012

Paesi

Modena

Roesch‐Ely

2015

Sci Med

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Aims: Rotavirus (RV) and norovirus (NoV) are the most common causes of diarrhea in children aged less than five years throughout the world. However, RV and NoV have frequently been overlooked as pathogens in elderly individuals. This study examined the frequency of RV and NoV among elderly patients with complaints of diarrhea in the city of Caxias do Sul, southern Brazil. Methods: Between 2010 and 2012, stool samples from patients aged 60 years or older with acute gastroenteritis treated at a private healthcare center were analyzed, seeking to identify RV and NoV. RV detection was performed by latex agglutination (LA) methods and polyacrylamide gel electrophoresis (PAGE). One third of the samples were further tested for NoV by reverse transcription polymerase chain reaction (RT-PCR). Results: A total of 145 stool samples from patients aged 60 to 105 years were analyzed. RV was detected in 6/145 (4.14%) and 5/125 (4.00%) of the samples by LA and PAGE, respectively. NoV was detected in 6/51 (11.76%). Mixed infection (RV and NoV) was detected in a single sample: 1/51 (1.96%). Conclusions: This study adds further evidence that viral agents are involved in the etiology of gastroenteritis in the elderly, contributing significantly to the understanding of RV and NoV infections in the mature population.

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“…According to Jurzik et al (2010) norovirus infected 344 and 1,699 people, respectively, in southern Italy and in Podgorica (Montenegro) due to contaminated water, and, additionally, 460 people were infected with enterovirus (EV) during an outbreak in the Republic of Belarus. In Brazil, Morillo et al (2008) also showed the importance of norovirus in waterborne infection.…”

Section: Waterborne Viral Pathogensmentioning

confidence: 99%

Waterborne Viral Pathogens: Detection, Control and Monitoring of Water Quality for Human Consumption

Faccin-Galhardi¹,

Lopes²,

Espada³

et al. 2013

VR&R

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The viral contamination of water for human consumption is a common cause of diseases outbreaks, therefore, a matter of great relevance for public health, especially in developing countries. In most countries, legislation recommends the study of coliforms to indicate the potability of water, however, this is not correlated with the presence of virus. The present review sought to characterize the epidemiological importance of waterborne viral diseases and the limitations of laboratorial detection and elimination of these pathogens. Besides, the state of art of virus as a tool to assess the quality of the water, as well as, the use of a single virus as the indicator of environmental contamination by virus itself are presented. Reports have demonstrated that some viruses are resistant to conventional water treatment process. Changes in water treatment policy should be achieved, and, should involve viral detection and resistance to physical and chemical agents, amongst other methods. However, we have to bear in mind that the decrease of waterborne infectious diseases is greatly dependent upon the improvement of sanitation, water treatment, together with preventive measures. All those measures together could ensure appropriate conditions for the promotion of human health and the reduction of environmental impacts.

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Identification and Molecular Characterization of Norovirus in São Paulo State, Brazil

Cited by 18 publications

References 28 publications

Characterization of rotavirus and norovirus strains: a 6-year study (2004-2009)

Characterization of rotavirus and norovirus strains: a 6-year study (2004-2009)

Detecção de rotavírus e norovírus na população idosa na cidade de Caxias do Sul, Rio Grande do Sul, Brasil, de 2010 a 2012

Waterborne Viral Pathogens: Detection, Control and Monitoring of Water Quality for Human Consumption

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