Microbiological and histopathological aspects of canine pyometra

Abstract

As pyometra is recognized as one of the main causes of disease and death in the bitch the purposes of this study were to evaluate microbiological and histopathological aspects of canine pyometra and to research the virulence factors of the E. coli isolates identifying possible risks to human health. The microbiological isolation from the intrauterine contents of 100 dogs with pyometra was carried out and the virulence factors in the E. coli strains were identified using PCR method. This study also consisted of the counting of microorganisms colonies forming units in samples of intrauterine content, tests of antimicrobial susceptibility of the E. coli isolates and the histological examination of the uterus. E. coli was the most prevalent microorganism isolated (76.6%) and 120 strains (79.5%) were positive for sfa, 86 (56.9%) were positive for cnf, 87 (57.6%) were positive for pap, 52 (34.4%) were positive for hly, 51 (33.8%) were positive for iuc and 5 (3.3%) were positive for afa genes. One observed more sensitivity of E. coli to norfloxacin, polimixin B, sulphazotrin, chloranfenicol and enrofloxacin. In 42% of the samples of uterine walls where microorganisms were isolated, the sizes of the areas of the inflammatory responses corresponded to 39-56%. Virulence factors were identified in 98.0% of the strains evaluated, demonstrating a high frequency of potentially pathogenic E. coli. It must be considered that dogs are animals that are living in close proximity to man for thousands of years and have an important role in the transmission of E. coli to other animals and to man.

Köpeklerde Endotoksik Pyometranın Klinik, Bakteriyolojik ve Histopatolojik Özellikleri

“…It has been established that if E. coli migrates to the uterus, it binds with higher affinity to the endometrium and myometrium layers and constitutes a resistance to the uterus' defense mechanisms such as lactoferrin and mucin-1 [24][25][26][27] . As reported in previous studies [4,11,23,28] , gram negative bacteria (75%) such as E. coli, Klebsiella spp. and Ps.…”

“…aeruginosa, Proteus spp., Pasteurella spp. could also been detected [11,22,23] . It has been established that if E. coli migrates to the uterus, it binds with higher affinity to the endometrium and myometrium layers and constitutes a resistance to the uterus' defense mechanisms such as lactoferrin and mucin-1 [24][25][26][27] .…”

Screening Canine Microbiota for Extended Spectrum Beta-Lactamases (ESBLs) Using E-Test

“…In a study by Bassessar et al, (2013) from Madhya Pradesh, E. coli was isolated predominantly (50%) from canine pyometra cases followed by Salmonella (20%), Staphylococcus (20%) and Pseudomonas (10%) species. Coggan et al, (2008) from Brazil reported 74.1% incidence of E. coli in canine pyometra cases. Microbiota isolated from otitis pus samples includes Pseudomonas (8, 53.3%), Proteus vulgaris (9, 60%) and Staphylococcus (15, 100%) species.…”

Microbiota cérvico-vaginal de ovelhas mestiças e sua susceptibilidade aos antibióticos

“…Entretanto, S. epidermidis foi isolado de seis animais, corroborando com os achados do presente estudo. O isolamento de S. schleiferi na mucosa genital já foi descrita em algumas espécies como em jabutis (Pessoa 2009), cães (Coggan et al 2008) e em humanos (Campos et al 2008), entretanto são escassos estudos referentes a este microrganismo no sistema genital de ovelhas. Bactérias do gênero Micrococcus são descritas como habitantes das mucosas de diversas espécies animais (Gomes 2006, Fornazari et al 2007, Uchôa et al 2009), sendo considerado de baixa patogenicidade (Wunder et al 1976).…”