In vitro synergistic activity of antibiotic combinations against Brucella melitensis using E-test methodology

Kılıç, Selçuk; Dizbay, Murat; Hızel, Kenan; Arman, Dilek

doi:10.1590/s1517-83822008000200006

Cited by 13 publications

(32 citation statements)

References 28 publications

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“…In our study MIC 50 and MIC 90 values for TMP-SMZ were lower than those previously observed in Turkey 8,10,11 and conforming the results of Kilic et al 16. In vitro TMP-SMZ resistance rate was reported 2% in Turkey 8.…”

Section: Discussionsupporting

confidence: 91%

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Antimicrobial Susceptibilities of Brucella Isolates from Various Clinical Speciemens

Bayram¹,

Körkoca²,

Aypak³

et al. 2011

Int. J. Med. Sci.

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Purpose: Brucellosis is a worldwide zoonotic disease and still constitutes a major public health problem. In the study we claimed to identify Brucella species from clinical samples of patients with active brucellosis from Van region of Eastern Anatolia and to determine in vitro antimicrobial susceptibilities of these strains to commonly used anti-Brucella agents and a possible new alternative tigecycline.Materials and Methods: A total of 56 Brucella isolates were enrolled the study and the identification of the isolates were based on conventional methods. In vitro activities of antimicrobials were evaluated by the E test method.Results: All isolates were identified as B. melitensis. MIC90 values of doxycycline, streptomycin, rifampin, trimethoprim-sulfamethoxazole and tigecycline were 0.064 mg/L, 1 mg/L, 2 mg/L, 0.125 mg/L and 0.094 mg/L, respectively. Tigecycline had low MIC50 and MIC90 values against all B. melitensis strains; the highest MIC observed was 0.25 μg/mL.Conclusion: Our data suggest that tigecycline can be a therapeutic alternative option for the treatment of brucellosis.

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Section: Discussionsupporting

confidence: 91%

“…Fluoroquinolones, macrolides and tigecycline (TIG), a member of a new class of antimicrobials, the glycylcyclines, may serve as alternative drug choices 12-16.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Susceptibilities of Brucella Isolates from Various Clinical Speciemens

Bayram¹,

Körkoca²,

Aypak³

et al. 2011

Int. J. Med. Sci.

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“…Although the E-test method used to determine the synergistic effects of antimicrobials and in-vitro sensitivities is an appropriate method, a standard synergy test method has not been developed (7,12,20,26). In our study, the highest in-vitro synergistic efficacy was determined against DOX-RIF (100%) combination as Kılıç et al (12) did with E-test combination method. The DOX-RIF synergic efficacy was found to be 94% by the E-test method and 63% by the checkerboard method by Orhan et al Akova et al (27) used 20 Brucella spp.…”

Section: Discussionmentioning

confidence: 72%

“…The most sensitive antimicrobial agent in Turkey is reported as SXT. Kilic et al (12) conducted a study of SXT MIC50 value as 0.064 μg/ml, and MIC90 value was found to be 0.094 μg/ml. Baysan et al (13) reported SXT MIC50 and MIC90 as 0.047 μg/ml and 0.094 μg/ml, respectively; whereas Ascen et al (14) reported as 0.012 μg/ml and 0.023 μg/ml orderly.…”

Section: Discussionmentioning

confidence: 99%

Investigation of Synergistic, Additive and Antagonist Effect of Antimicrobial Combinations Used for Brucella spp., with E-test Combination Method

Kaysadu¹,

Duman²,

Yakupoğulları³

2019

Eastern J Med

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Brucella spp. is a significant cause of morbidity and mortality in human. Though combination therapy is recommended in the patients with brucellosis, there is very less data about the in vitro efficacy of the combined antimicrobials. In this study, we aimed to investigate the in vitro activities of antimicrobial combinations used against the Brucella spp. strains. A total 40 human isolates of Brucella spp. strains were included in this study. In vitro activities of doxycycline, rifampin, cotrimoxazole, streptomycin and ciprofloxacin were studied by E-test (Bio Mérieux, France) strips. In vitro effectiveness of doxycycline-rifampin, rifampin-cotrimoxazole, doxycycline-streptomycin, ciprofloxacin-cotrimoxazole, and ciprofloxacin-streptomycin were investigated by E-test combination method, and the results were evaluated with Fractional Inhibitor Concentration Index. Cotrimoxazole was found as the most active antimicrobial against to tested Brucella isolates with the lowest Minimal Inhibitory Concentration (MIC) (as, 0.016 µg/ml), while rifampin was the least active drug with highest MIC (as 1.5 µg/ml). The MIC50 and MIC90 values of the tested antibiotics were as f ollows: cotrimoxazole 0.032 μg/ml and 0.064 μg/ml, doxycycline 0.064 μg/ml and 0.094 μg/ml, ciprofloxacin 0.094 μg/ml and 0.75 μg/ml, streptomycin 0.25 μg/ml and 0.50 μg/ml, and rifampin 0.50 μg/ml and 0.75 μg/ml, respectively. Doxycycline-rifampin combination showed a remarkable synergistic activity to all tested strains (%100), but rifampin-cotrimoxazole combination exhibited antagonist activity to two strains (5%). The ratios of synergistic activities were as f ollows: ciprofloxacin-streptomycine 57.5%, rifampin-cotrimoxazole 52.5%, doxycycline-streptomycin 32.5%, and ciprofloxacin-cotrimoxazole 25%. In this study, though cotrimoxazole was the highest in vitro active against to tested strains, its combination showed low synergistic effect with other antimicrobials. On the contrary, though rifampin showed low in vitro activity alone, it exhibited excellent synergistic effect when combined with doxycycline. Therefore, w hen the treatment is planned for a patient with Brucellosis, it will be benefit for testing the combination effectiveness of the drugs irrespective of their in vitro activities alone.

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“…The combination between the rifampicin and trimethoprim-sulfomethoxazole is the suggested regimen for children (3, 15, 16). Triple-antibiotic combinations were found to be of value in some cases of brucellar endocarditis, meningitis, and spondylitis (2, 21, 31).…”

Section: Introductionmentioning

confidence: 99%

Efficacy evaluation of some antibiotics against syrian brucella spp isolates, in vitro

Safi

Al-Mariri

2012

Braz. J. Microbiol.

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Brucellosis is an endemic zoonosis in Syria, affecting large numbers of animals and there are an increasing number of cases in humans. The aim of this study is to investigate the in vitro efficacy of various traditional and new antibiotics against 89 Brucella isolates (isolated from domestic animals) collected from different Syrian regions. Minimum inhibitory concentrations (MICs) of seventeen antibiotics were determined. Ciprofloxacin and ofloxacin were the most effective antibiotics, whereas sparfloxacin, levofloxacin, doxycycline and tetracycline had a moderate activity. In contrast, moxifloxacin and rifampicin had a low activity, while streptomycin, spiramycin and cephalosporines were ineffective. As a result, we come to the conclusion that a combination between one effective quinolone and doxycycline has a good efficacy against Brucella. Further in vivo studies are necessary to support this suggestion.

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In vitro synergistic activity of antibiotic combinations against Brucella melitensis using E-test methodology

Cited by 13 publications

References 28 publications

Antimicrobial Susceptibilities of Brucella Isolates from Various Clinical Speciemens

Antimicrobial Susceptibilities of Brucella Isolates from Various Clinical Speciemens

Investigation of Synergistic, Additive and Antagonist Effect of Antimicrobial Combinations Used for Brucella spp., with E-test Combination Method

Efficacy evaluation of some antibiotics against syrian brucella spp isolates, in vitro

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