Antimicrobial efficacy of secondary metabolites from Glomerella cingulata

Kishore, K. Hara; Misra, Sunil; Chandra, Deepika; Prakash, K. Vanitha; Murty, Upadhyayula Suryanarayana

doi:10.1590/s1517-83822007000100031

Cited by 7 publications

(4 citation statements)

References 9 publications

(9 reference statements)

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“…Fungi are known to play an important role in biotechnology applications and their SMs have recently been used as effective biological pesticides [49]. Most of the antimicrobial metabolites are mainly derived from soil-borne fungi and these microorganisms are less explored [50]. Analysis of antimicrobial activity of these metabolites against pathogens can be employed as a simple and efficient screening tool and could be used to isolate potentially useful strains and to further identify their bioactive agents [51].…”

Section: Discussionmentioning

confidence: 99%

Bioactive Secondary Metabolites from Trichoderma spp. against Phytopathogenic Bacteria and Root-Knot Nematode

et al. 2020

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Losses in crops caused by plant pathogenic bacteria and parasitic nematode are increasing because of a decrease in efficacy of traditional management measures. There is an urgent need to develop nonchemical and ecofriendly based management to control plant diseases. A potential approach of controlling plant disease in the crops is the use of biocontrol agents and their secondary metabolites (SMs). Luckily fungi and especially the genus Trichoderma comprise a great number of fungal strains that are the potential producer of bioactive secondary metabolites. In this study secondary metabolites from ten Trichoderma spp. were evaluated for their antibacterial and nematicidal potential against phytopathogenic bacteria Ralstonia solanacearum, Xanthomonas compestris and plant parasitic nematode Meloidogyne incognita. Five different growth media were evaluated for the production of SMs. It was shown that SMs of different Trichoderma spp. obtained on different growth media were different in the degree of their bioactivity. Comparison of five growth media showed that SMs produced on solid wheat and STP media gave higher antibacterial activity. SMs of T. pseudoharzianum (T113) obtained on solid wheat media were more effective against the studied bacteria followed by SMs from T. asperelloides (T136), T. pseudoharzianum (T129) and T. pseudoharzianum (T160). Scanning electron microscopy (SEM) was further conducted to observe the effect of SMs on bacterial cell morphology. As evident from the SEM, SMs produced severe morphological changes, such as rupturing of the bacterial cell walls, disintegration of cell membrane and cell content leaking out. SMs from T. viridae obtained on liquid STP and solid wheat media showed the highest percent of M. incognita juveniles (J2s) mortality and inhibition in egg hatching of M. incognita. The results of our study suggest that T. pseudoharzianum (T113) and T. viridae could be selected as an effective candidate for SMs source against phytopathogenic bacteria and M. incognita respectively.

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Section: Discussionmentioning

confidence: 99%

Bioactive Secondary Metabolites from Trichoderma spp. against Phytopathogenic Bacteria and Root-Knot Nematode

et al. 2020

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“…Fungi are recognized as prolific producers of secondary metabolites which have high therapeutic value as antibiotics, cytotoxic substances, insecticides, compounds that promote or inhibit growth, attractors and repellents, besides their biotechnological applications (Demain, 1999;Kishore et al, 2007;Mabrouk et al, 2008;Abdel-Aziz et al, 2018). Entomopathogenic fungi are classified as fungi that infect, invade and eventually kill their insects (Singkaravanit et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Snailicidal, antimicrobial, antioxidant and anticancer activities of Beauveria bassiana, Metarhizium anisopliae and Paecilomyces lilacinus fungal extracts

Abdel–Wareth¹,

Ghareeb²,

Abdel‐Aziz³

et al. 2019

Egypt. J. of Aquatic Biolo. and Fish.

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Fungi are capable of producing a wide variety of secondary metabolites. Entomopathogenic fungi along with species which parasitize nematodes represent excellent candidates for bioactive compounds production. This study aimed at exploring the snailicidal, antimicrobial and antioxidant activities of ethyl acetate and acetone extracts of Beauveria bassiana, Metarhizium anisopliae and Paecilomyces lilacinus. Ethyl acetate and acetone extracts were prepared and tested against Biomphalaria alexandrina snails, their antioxidant and antimicrobial activities were also investigated. The anticancer activity of P. lilacinus acetone extract was tested on hepatocellular carcinoma (HepG2) cells. Acetone extract of P. lilacinus (LC 50 =120 ppm) was more toxic to B. alexandrina snails than that of B. bassiana (LC 50 = 231 ppm). Sublethal concentrations of both extracts adversely affected survival rate, histological pattern of digestive and hermaphrodite glands of the tested snails, besides their genotoxicity to DNA of snails. P. lilacinus acetone extract showed the highest antioxidant activity. Also, its antimicrobial activity against Staphylococcus aureus, Escherichia coli and Candida albicans was high. Moreover, it was cytotoxic to HepG2 cells (IC 50 =2.81 µg/ml). GC/MS analysis indicated the presence of 37 compounds in B. bassiana acetone extract with 2-methylhept-2,6-dien-4-ol and 1,3-dimethoxy-2-(hydroxymethyl)-9h-xanthene as major compounds. While 48 compounds were identified in P. lilacinus acetone extract where hexadecanoic acid, methyl ester and 10, 13 octadecadienoic acid, methyl ester were the major compounds. In conclusion, B. bassiana and P. lilacinus are excellent sources of bioactive compounds which have multiple biological effects. Pomacea canaliculata (Maketon et al., 2009). Generally, diverse toxic metabolites have been described in several fungal biological control agents including Beauveria, Metarhizium and Pacilomyces (Vey et al., 2001), some of these metabolites have been found to possess antibiotic, fungicidal or insecticidal properties (

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“…Soil is the most essential source for the isolation of fungi [15]. For example, species belonging to the genera Aspergillus, Penicillium and Mucor have been isolated from roots of soils in different agircultural sites.…”

Section: Molecular Identification and Evolutionary Relationship Of Somentioning

confidence: 99%

Antimicrobial Potentials of Soil Fungi Metabolites, Molecular Dynamics and Their 3d Protein Structural Prediction Using Bioinformatics Tool

Majolagbe

Aina

Omomowo

et al. 2020

Int J Curr Pharm Sci

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Objective: To determine the antimicrobial potentials of secondary metabolite of soil fungi and predict their 3D structure and molecular identity. Methods: Pure soil fungi were isolated from soil samples and cultured under submerged fermentation (Smf) for their metabolites using Potato Dextrose Agar and Broth. The secondary metabolites of the isolated fungi were obtained intracellularly after 21 d of incubation in a rotary shaker incubator. The antimicrobial potentials of the metabolites were investigated against four (4) clinical isolates, namely: Staphylococcus aureus, Klebsiella spp, Candida albicans and Escherichia coli. These soil fungi were further characterized to the molecular level and their evolutionary relationships established using bioinformatics tools. Protein structure of each of the fungi isolates was predicted using PHYRE-2. Results: Out of all the soil fungi isolated, the metabolite of Aspergillus aculeatus showed the highest antimicrobial activities against Staphylococcus aureus (23.00±2.34 mm), Escherichia coli (9.00±1.44 mm) and Klebsiella spp (24.00±3.45 mm). The 3D protein structure predicted showed that each of the organisms consists of different amino-acid compositions such as: serine, tyrosine, proline, arginine, glycine, phenylalanine leucine with other notable biological properties. Conclusion: The work revealed that secondary metabolites of the isolated fungi carry an important role in combating infectious agents thereby, providing roadmaps for the biosynthesis of many synthetic and semi-synthetic drugs and bio-products which are environmentally friendly.

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Antimicrobial efficacy of secondary metabolites from Glomerella cingulata

Cited by 7 publications

References 9 publications

Bioactive Secondary Metabolites from Trichoderma spp. against Phytopathogenic Bacteria and Root-Knot Nematode

Bioactive Secondary Metabolites from Trichoderma spp. against Phytopathogenic Bacteria and Root-Knot Nematode

Snailicidal, antimicrobial, antioxidant and anticancer activities of Beauveria bassiana, Metarhizium anisopliae and Paecilomyces lilacinus fungal extracts

Antimicrobial Potentials of Soil Fungi Metabolites, Molecular Dynamics and Their 3d Protein Structural Prediction Using Bioinformatics Tool

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