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Fungi have great prospects for synthesis, applications and developing new products in nanotechnology. In recent times, fungi use in nanotechnology is gaining more attention because of the ecological friendly state of their metabolite-mediated nanoparticles, their safety, amenability and applications in diverse fields. The diversity of the metabolites such as enzymes, polysaccharide, polypeptide, protein and other macro-molecules has made fungi a veritable tool for nanoparticles synthesis. Mechanism of fungal nano-biosynthesis from the molecular perspective has been extensively studied through various investigations on its green synthesized metal nanoparticles. Fungal nanobiotechnology has been applied in agricultural, medical and industrial sectors for goods and services improvement and delivery to mankind. Agriculturally, it has found applications in plant disease management and production of environmentally friendly, non-toxic insecticides, fungicides to enhance agricultural production in general. Medically, diagnosis and treatment of diseases, especially of microbial origin have been improved with fungal nanoparticles through more efficient drug delivery systems with great benefits to pharmaceutical industries. This review therefore explored fungal nanobiotechnology; mechanism of synthesis, characterization and potential applications in various fields of human endeavours for goods and services delivery.

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The present study aims to compare two traditional extraction techniques. A volatile compound from Tamarindus indica seed was obtained with Soxhlet extraction (SE) and hydrodistillation using the Clevenger apparatus (HDC). The extraction yield and chemical composition of the essential oil samples were compared. Both oils extracted were analyzed with GC-MS, and forty-one chemical compounds were identified in essential oil components from SE while forty chemical compounds were found in the HDC-extracted oil sample. The major essential oil components present in both the SE and HDC method are cis-vaccenic acid, 2-methyltetracosane, beta-sitosterol, 9,12-octadecadienoic acid (Z, Z)-, and n-hexadecanoic acid in varying concentrations. Moreover, the essential oils obtained by both methods look similar quantitatively but differ qualitatively. The HDC method produced more oxygenated compounds that contribute to the fragrance of the oil. The major constituents observed in the essential oil extracted by SE methods include cis-vaccenic acid (17.6%), beta-sitosterol (12.71%), 9,12-octadecadienoic acid (Z, Z)- (11.82%), n-hexadecanoic acid (8.16%), 9,12-octadecadienoic acid, methyl ester (5.84%), oleic acid (4.54%), and 11-octadecenoic acid and methyl ester (3.94%). However, in the hydrodistillation technique, the oil was mostly composed of 9,12-octadecadienoic acid (Z, Z)- (23.72%), cis-vaccenic acid (17.16%), n-hexadecanoic acid (11.53%), beta-sitosterol (4.53%), and octadecanoic acid (3.8%). From the data obtained, HDC seems to be a better method for extraction of Tamarindus indica essential oil compared to the Soxhlet extraction apparatus.

Aqueous leaf extract of Hyptis suaveolens was investigated to synthesize silver nanoparticles (Hs-AgNPs). Biomolecules such as phenolics, proteins, carbohydrates and esters facilitated bioformation of Hs-AgNPs with ƛmax at 420 nm. The nearly spherical Hs-AgNPs were polydispersed and sized 29.19-52.27 nm with yield of 85.8 %. Hs-AgNPs showed potent inhibition of 75.22-100 % against multidrug resistant bacteria, and fungal growth inhibition of 73.33-100 %. Hs-AgNPs scavenged DPPH and H2O2 by 77.75-83.19, and 54.21-70.11 % respectively. It also effectively inhibited coagulation of blood. The study established the nanobiotechnological importance of H. suaveolens, with bioformation of AgNPs that have potent activities for environmental and biomedical applications.