Carbon catabolite repression of retamycin production by Streptomyces olindensis ICB20

Abstract

Retamycin is an anthracyclinic antitumoral complex produced by Streptomyces olindensis ICB20. In this work the influence of different glucose concentrations in the feed medium on the production of retamycin was studied. Chemostat cultures employing glucose concentration varying between 10 g/L and 25 g/L showed that use of high glucose concentration resulted in catabolite repression of the biosynthesis of the antitumoral. The highest specific retamycin production rate, qRTM = 7.8 mg/g.h, was obtained when glucose concentration was 10 g/L. The lowest value of qRTM, 2.5 mg/g.h, was observed when glucose concentration was 20 and 25 g/L. The residual glucose concentration varied from 0 to 13 g/L, as the glucose concentration in the feed was increased from 10 to 25 g/L.

A Genome-Scale Insight into the Effect of Shear Stress During the Fed-Batch Production of Clavulanic Acid by Streptomyces Clavuligerus

“…Organic acid secretion in streptomycetes ( Figure 2 b–g) is related to an imbalance in carbon fluxes between glycolysis and the TCA cycle under carbon source abundance, also referred to as overflow metabolism [ 45 , 46 ]. Acid secretion can be also related with the activation of secondary metabolism in some Streptomyces species like S. coelicolor and S. clavuligerus [ 10 , 47 ].…”

Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge Isolate Streptomyces sp. SM17 When Compared to Its Terrestrial Relative S. albidoflavus J1074

“…Firstly, it appears likely that surugamide A biosynthesis may be regulated, at least in part, by carbon catabolite repression (CCR). Carbon catabolite repression is a well-described regulatory mechanism in bacteria that controls carbon metabolism [79,80,81,82], and which has also been reported to regulate the biosynthesis of secondary metabolites in a number of different bacterial species, including in Streptomyces isolates [83,84,85,86]. While the TSB and the YD media contain glucose and dextrins as carbon sources, respectively; the complex polysaccharide starch is the carbon source in the SYP-NaCl medium.…”

Carbon source regulation of antibiotic production

“…Under these conditions, an increase in the excretion of organic acids (such as pyruvate, citrate, succinate and lactate), with reduction in the biomass yield is observed, suggesting that flux through the glycolytic pathway has an important role in the repression of the biosynthesis of this antitumoral agent. 92 Doxorubicin also belongs to the anthracycline family of antitumor compounds. Its synthesis can be hindered by glucose and galactose in Streptomyces peucetius var.…”